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5' RACE Protocol For Generation of Seq. Tags - http://baygenomics.ucsf.edu/protocols/comp1/RACE_generation.pdf

Category: RNA Protocols: 5' RACE - Rapid Amplification of cDNA Ends Protocols

Can also use 96-well plates. First Strand cDNA Synthesis, First Size selection, second 2nd strand cDNA synthesis. Bay Area Functional Genomics Consortium, UCSF. - [Read 5' RACE Protocol For Generation of Seq. Tags]

Actin Capture Assay Protocol - http://genomics.princeton.edu/botstein/protocols/actin_capture.html

Category: Cytoskeleton Protocols: Actin Myosin Protocols

Protocol for actin capture assay. Includes: Actin Capture Assay Reagants. - [Read Actin Capture Assay Protocol]

Actin Capture Assay Protocol - http://genomics.princeton.edu/botstein/protocols/actin_capture.html

Category: Yeast Protocols

Protocol for actin capture assay. - [Read Actin Capture Assay Protocol]

Antibody Microarrays Are a Powerful Tool for Proteomics - http://www.genpromag.com/ShowPR.aspx?PUBCODE=018&ACCT=1800000100&ISSUE=0505&RELTYPE=PR&ORIGRELTYPE=MIC&PRODCODE=00000000&PRODLETT=Z

Category: Antibody Microarray Protocols

Antibody Microarrays Are a Powerful Tool for Proteomics. Genomics and Proteomics - [Read Antibody Microarrays Are a Powerful Tool for Proteomics]

DNA Electroelution - http://rothlab.ucdavis.edu/protocols/dna-electroelution.html

Category: DNA Protocols: DNA Extraction Protocols: DNA Extraction Polyacrylamide Gels

DNA Electroelution. This protocol describes the purification of DNA by trapping in a high-salt cushion in a "UEA AnalyticalElectroeluter" (IBI). This machine is no longer manufactured, to our knowledge. However, a smiliar device can be easily made from Plexiglas according to the following diagram, taken from Cornel Mulhardt, Molecular Biology and Genomics (2007) Academic Press, p.52: Schimenti Lab - [Read DNA Electroelution]

DNA Prep for CGH Protocol - http://www.genomics.princeton.edu/dunham/MDyeastDNAprep.htm

Category: Yeast Protocols: Yeast Nucleic Acid Protocols: Yeast DNA Isolation Protocols

Protocol for DNA prep for CGH. - [Read DNA Prep for CGH Protocol]

FROZEN SECTIONING PROTOCOL Plant Tissue PDF - http://schnablelab.plantgenomics.iastate.edu/docs/resources/protocols/Cryosectioning_for_LCM.pdf

Category: Histology Protocols: Tissue Sample Sectioning

PREPARATION OF PLANT TISSUE FOR LASER MICRODISSECTION. Schnable Labs, Plant Genomics. - [Read FROZEN SECTIONING PROTOCOL Plant Tissue PDF]

Harvesting for RNA - http://www.genomics.princeton.edu/dunham/MDyeastRNA.htm

Category: Yeast Protocols: Yeast Nucleic Acid Protocols: Yeast RNA Isolation Protocols

Harvesting for RNA. Includes: RNA prep; Small RNA prep; Large RNA prep. - [Read Harvesting for RNA]

Lithium Acetate Transformation of Yeast Protocol - http://www.genomics.princeton.edu/dunham/yeasttransformation.htm

Category: Yeast Protocols: Yeast Transformation Protocols

Protocol for lithium acetate transformation of yeast. - [Read Lithium Acetate Transformation of Yeast Protocol]

Microarray troubleshooting: image gallery - http://stress-genomics.org/stress.fls/expression/array_tech/trouble_shooting/troubles_index.htm

Category: DNA Microarray Protocols: Troubleshooting DNA Microarray

Images present some common problems encountered during microarray production, with possible solutions. Galbraith laboratory. - [Read Microarray troubleshooting: image gallery]

Paraffin Sectioning for LCM Plant Tissue - http://schnablelab.plantgenomics.iastate.edu/docs/resources/protocols/Paraffin_sectioning_for_LCM.pdf

Category: Histology Protocols: Tissue Sample Sectioning

FIXATION, DEHYDRATION, PARAPLAST INFILTRATION AND SECTIONING PROTOCOL WITH TISSUE COLLECTION PROCEDURE USING THE PALM LASER MICRODISSECTION SYSTEM. Schnable Labs, Plant Genomics. - [Read Paraffin Sectioning for LCM Plant Tissue]

SDS-PAGE An Introduction - http://www.bio.davidson.edu/courses/genomics/method/SDSPAGE/SDSPAGE.html

Category: Protein Protocols: Protein Electrophoresis

SDS-PAGE An Introduction. A great introduction to SDS-PAGE for first time users. - [Read SDS-PAGE An Introduction]

Sporulation Guide - http://www.genomics.princeton.edu/dunham/sporulationdissection.htm

Category: Yeast Cell Culture Protocols: Yeast Sporulation Protocols

Protocols and Information on sporulation. Includes: SPO++ media; 10X amino acid stock; Sporulation; Dissection. - [Read Sporulation Guide]

TaqManÂ® SNP Genotyping Assays - http://www.rockefeller.edu/genomics/pdf/Applied_Biosystems_Taqman_SNP_Genotyping.pdf

Category: DNA Protocols: SNP Protocols

TaqManÂ® SNP Genotyping Assays. Applied Biosystems - [Read TaqManÂ® SNP Genotyping Assays]

Understanding Protein Trafficking in Plant Cells Through Proteomics - http://www.cepceb.ucr.edu/resources/pdf/pan/ERP-review2_781_2005.pdf

Category: Protein Protocols: Protein Trafficking

Background and methods to study plant transport. Includes new methods to study protein trafficking in plant cells, includes: Identification of protein sorting pathways in non purified samples; Localization of organelle proteins by isotype tagging/isotype-coded affinity tag; Coupling of chemical genomics and proteomics; Top down mass spectrometry; Compartment-specific markers to aid in the purification of organelles. - [Read Understanding Protein Trafficking in Plant Cells Through Proteomics]

Vectorette PCR of Yeast DNA Protocol - http://genomics.princeton.edu/botstein/protocols/vectorette.html

Category: Yeast Protocols: Yeast Nucleic Acid Protocols: Yeast PCR Protocols

Protocol for vectorette PCR of Yeast DNA. - [Read Vectorette PCR of Yeast DNA Protocol]

Yeast IF without Dehydration - http://genomics.princeton.edu/botstein/protocols/non_MeOH_IF.html

Category: Molecular Imaging: Immunofluorescence Microscopy

Yeast IF without Dehydration. Botstein Lab. Protocol is a modified version of the protocol from Mark Rose in the CSH Yeast Genetics Course Manual. - [Read Yeast IF without Dehydration]

Yeast Media Protocols - http://genomics.princeton.edu/botstein/protocols.shtml

Category: Yeast Cell Culture Protocols: Yeast Media, Solutions and Stocks Protocols

Protocols for yeast media including: YNB1 1.1x agar, YNB2 1.1x liquid, YNB3 agar, YNB4 liquid, YNB5 Agarose, YP1 1.1x agar pH6, YP2 1.1x agar, YP3 1.1x liquid pH6, YP4 1.1x liquid, YPD1 agar pH6, YPD2 agar, YPD3 liquid pH6, YPD4 liquid. - [Read Yeast Media Protocols]