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Southern Blotting: Capillary Transfer of DNA to Membranes Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot4040

Category: Nucleic Acid Detection Protocols: Nucleic Acid Blotting Protocols

Protocol describes the first stages of Southern blotting: digestion of genomic DNA with one or more restriction enzymes, separation of the resulting fragments by electrophoresis through an agarose gel, and transfer of the denatured fragments to a membrane by downward capillary transfer. - [Read Southern Blotting: Capillary Transfer of DNA to Membranes Protocol]

Synthesis of dsRNA for RNAi in Drosophila: Plasmid Template Method Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/21/pdb.prot4512

Category: RNAi Technology Protocols: RNAi Drosophila Protocols

Protocol describes how to prepare double-stranded RNA (dsRNA) for RNA interference in Drosophila by synthesis of individual RNA strands from linearized plasmid templates, followed by annealing of the strands. DsRNA molecules with a length of 500-800 bp seem to be most active. The dsRNA can be made from cDNA or genomic DNA templates, as long as most of the dsRNA corresponds to presumptive exon sequence. - [Read Synthesis of dsRNA for RNAi in Drosophila: Plasmid Template Method Protocol]

Tail DNA Preps for Genomic Southerns Protocol - http://members.cox.net/microinjectionworkshop/technical/tnp10.html

Category: Mouse Protocols: Mouse Genetics Protocols

Protocol for tail DNA preps for genomic southerns. - [Read Tail DNA Preps for Genomic Southerns Protocol]

Use of PCR for Quality Control of a Genomic DNA Library Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4138

Category: DNA Protocols: Genomic DNA Library Protocols

Protocol describes the use of PCR to analyze the quality of a human genomic DNA library. It has been optimized for a human genomic DNA library containing fragments between approximately 50 and 500 bp. - [Read Use of PCR for Quality Control of a Genomic DNA Library Protocol]

Yeast Genomic DNA Isolation Procedure - http://www.upstate.edu/biochem/amberg/protocols/yeast_genomic_DNA.html

Category: DNA Protocols: DNA Extraction Protocols

Yeast Genomic DNA Isolation David Amberg, Upstate Medical University. - [Read Yeast Genomic DNA Isolation Procedure]

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Populational Analysis of Genomic DNA Protocol

A protocol on the populational Analysis of genomic DNA.

Genomic DNA Labeling of Microarrays Protocol

DNA microarrays are an ordered arrangement of DNA molecules complementary to genes of interest that are "spotted" by robotic equipment onto a glass slide substrate. The expression of genes in cells can be monitored with microarrays by preparing cDNA from the mRNA of cells of interest and measuring the hybridization to the microarray. This protocol describes the labeling of genomic DNA for use as a probe for hybridization to the cDNA spotted on the array.

Vector Design Protocol for Transgenic Mice Generation

The protocol gives general considerations for the design of targeting vectors for transgenic mice. The protocol shares tips in the design of knock-out and knock-in vectors and some of their strategies for producing homologously recombined embryonic stem cells.

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