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DNA extraction from blood - http://genome-lab.ucdavis.edu/Protocols/dna_extraction_from_blood.htm

Category: DNA Protocols: DNA Extraction Blood

Purifying nuclear pellets, Extraction of DNA from nuclei. Method for DNA isolation from Blood. Genomic Variation Laboratory, UC Davis. - [Read DNA extraction from blood]

DNA IQ Isolation of Genomic DNA from Stains and Buccal Swabs Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4098

Category: Nucleic Acid Purification Protocols: DNA Isolation Protocols: Genomic DNA Isolation Protocols

In this protocol, the DNA-binding capacity of Wizard MagneSil particles is used to capture and release a consistent amount of DNA (100 ng) across a wide range of samples. At the end of the procedure, the DNA is eluted into 100 µl Elution Buffer to give a final concentration of 1 ng/µl, relieving the need for postpurification DNA quantitation. - [Read DNA IQ Isolation of Genomic DNA from Stains and Buccal Swabs Protocol]

DNA preparation by cryotom tissue dissection - http://amba.charite.de/cgh/protocol/02/mapeng02.htm

Category: Cytogenetics Protocols: Comparative Genomic Hybridization CGH Protocols

DNA preparation by cryotom tissue dissection for CGH Comparative Genomic Hybridization. Institute of Pathology "Rudolf- Virchow-Haus" University Hospital CharitÃ© - [Read DNA preparation by cryotom tissue dissection]

Drosophila Genomic DNA Preparation Protocol - http://vosshall.rockefeller.edu/protocols/Flygenomic.pdf

Category: Drosophila Protocols: Drosophila Nucleic Acids Protocols

Protocol for Drosophila genomic DNA preparation. - [Read Drosophila Genomic DNA Preparation Protocol]

Drosophila Single Fly Genomic DNA Prep Protocol - http://vosshall.rockefeller.edu/protocols/SingleFlyGenomic.pdf

Category: Drosophila Protocols: Drosophila Nucleic Acids Protocols

Protocol for Drosophila single fly genomic DNA prep. - [Read Drosophila Single Fly Genomic DNA Prep Protocol]

Electroporation of the Library into COS-7 Cells for Exon Trapping and Amplification - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3733

Category: DNA Protocols: Genomic DNA Library Protocols

COS-7 cells are transfected with a library of plasmids containing the genomic DNA of interest. - [Read Electroporation of the Library into COS-7 Cells for Exon Trapping and Amplification]

Exon Trapping and Amplification for Construction of the Library Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot4075

Category: DNA Protocols: Genomic DNA Library Protocols

Protocol describes how to construct and amplify a genomic DNA library in a plasmid vector (pSPL3) equipped to express cloned exons in transfected mammalian cells. - [Read Exon Trapping and Amplification for Construction of the Library Protocol]

Fan Lab Bisulfite Treatment of Genomic DNA - http://www.genetics.ucla.edu/labs/fan/Protocols_Bisulfite.htm

Category: Epigenetics and Methylation Protocols: Bisulfite Treatment Methylation Assay Protocol

A detailed protocol for bisulfite treatment of DNA. DNA is first digested with a restriction enzyme. DNA is denauted at 97C for 5min. Bisulfite protocol is included. Also DNA purification using the Promega Wizard DNA Cleanup kit in indicated. Fan Lab. UCL - [Read Fan Lab Bisulfite Treatment of Genomic DNA]

Genomic DNA isolation from blood - http://www.genome.ou.edu/protocol_book/protocol_partIII.html#III.H

Category: DNA Protocols: DNA Extraction Blood

Genomic DNA isolation from blood. Roe Lab - [Read Genomic DNA isolation from blood]

Genomic DNA Labeling Protocol - http://cmgm.stanford.edu/pbrown/protocols/4_genomic.html

Category: Microarray Protocols: Microarray DNA Labeling Protocols

Protocol for genomic DNA labeling. - [Read Genomic DNA Labeling Protocol]

Genomic DNA Labeling Protocol - http://www.genome.wisc.edu/resources/protocols/genomiclabeling.htm

Category: Nucleic Acid Modification Protocols: Nucleic Acid Labeling Protocols

Protocol for genomic DNA labeling. - [Read Genomic DNA Labeling Protocol]

Genomic DNA Labeling Protocol II - http://cmgm.stanford.edu/pbrown/protocols/4_genomic.html

Category: Nucleic Acid Modification Protocols: Nucleic Acid Labeling Protocols

Protocol for genomic DNA labeling. - [Read Genomic DNA Labeling Protocol II]

Growth of S. cerevisiae and Preparation of DNA Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3298

Category: Yeast Protocols: Yeast Genetics Protocols: YACs Protocols

Protocol describes methods for isolation of DNA from a strain of S. cerevisiae carrying a recombinant YAC. Because the linear YAC DNAs are sensitive to shearing forces, pipettes with wide-bore tips should be used to transfer DNAs. The method is suitable for preparing DNA that will be used for agarose gel electrophoresis, Southern blotting, subcloning, genomic library construction, PCR, or other methods that do not require intact high-molecular-weight DNA. - [Read Growth of S. cerevisiae and Preparation of DNA Protocol]

Harvesting the mRNA for Exon Trapping and Amplification - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3734

Category: DNA Protocols: Genomic DNA Library Protocols

Standard techniques are used here to isolate cytoplasmic RNA from COS cells transfected with a library of recombinant plasmids containing the genomic DNA of interest. - [Read Harvesting the mRNA for Exon Trapping and Amplification]

Inverse PCR Protocol II - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3487

Category: PCR Protocols: Inverse PCR Protocols

Inverse PCR is used to amplify and clone unknown DNA that flanks one end of a known DNA sequence and for which no primers are available. The technique involves digestion by a restriction enzyme of a preparation of DNA containing the known sequence and its flanking region. The individual restriction fragments (many thousands in the case of total mammalian genomic DNA) are converted into circles by intramolecular ligation, and the circularized DNA is then used as a template in the PCR. - [Read Inverse PCR Protocol II]

Isolating the Ends of Genomic DNA Fragments Cloned in High-capacity Vectors - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot4009

Category: PCR Protocols: PCR Cloning Protocols

Protocol describes the use of vectorette PCR and single-site PCR to amplify the terminal sequences of genomic sequences cloned in high-capacity vectors such as PACs and YACs. - [Read Isolating the Ends of Genomic DNA Fragments Cloned in High-capacity Vectors]

Isolating Xenopus Genomic DNA Protocol - http://tropicalis.berkeley.edu/home/genetic_techniques/genomicDNA.html

Category: Xenopus Protocols: Xenopus Genetics Protocols

Protocol for isolating Xenopus genomic DNA. - [Read Isolating Xenopus Genomic DNA Protocol]

Isolation of DNA from Mammalian Cells by Spooling Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot4037

Category: Nucleic Acid Purification Protocols: DNA Isolation Protocols: DNA Isolation from Mammalian Cells Protocols

Procedure is used to prepare DNA simultaneously from many different types of samples or tissues. Although the DNA is generally too small (approx. 80 kb) for efficient construction of genomic DNA libraries, it gives excellent results in Southern hybridizations and PCRs. Cultured aneuploid mammalian cells (2 x 107, e.g., HeLa cells) yield 100 µg of DNA in a volume of 1 ml. - [Read Isolation of DNA from Mammalian Cells by Spooling Protocol]

Isolation of DNA from Mammalian Cells Grown in 96-well Microtiter Plates Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3695

Category: Nucleic Acid Purification Protocols: DNA Isolation Protocols: Genomic DNA Isolation Protocols

Protocol for isolation of DNA from mammalian cells grown in 96-well microtiter plates. Each well yields sufficient genomic DNA for several standard PCRs or for analysis in a single lane of a Southern hybridization. - [Read Isolation of DNA from Mammalian Cells Grown in 96-well Microtiter Plates Protocol]

Isolation of Genomic and Viral DNA from Lymphocytes Protocol - http://www1.qiagen.com/literature/protocols/pdf/QG03.pdf

Category: Viral Culture and Isolation Protocols: Viral Isolation Protocols

Protocol for the isolation of genomic and viral DNA from lymphocytes. Includes: Viral DNA in cytosolic fraction and Viral DNA in nuclear fraction integrated. - [Read Isolation of Genomic and Viral DNA from Lymphocytes Protocol]

Isolation of Genomic and Viral DNA from Lymphocytes using the QIAGEN® Genomic-tip - http://www1.qiagen.com/literature/protocols/pdf/QG03.pdf

Category: Nucleic Acid Purification Protocols: DNA Isolation Protocols: Genomic DNA Isolation Protocols

Protocol for the isolation of genomic and viral DNA from lymphocytes using the QIAGEN® Genomic-tip. - [Read Isolation of Genomic and Viral DNA from Lymphocytes using the QIAGEN® Genomic-tip]

Isolation of High-molecular-weight DNA from Mammalian Cells Using Proteinase K and Phenol Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot4036

Category: Nucleic Acid Purification Protocols: DNA Isolation Protocols: DNA Isolation from Mammalian Cells Protocols

Method of choice when large amounts of mammalian DNA are required, for example, for Southern blotting (Rapid Isolation of Mammalian DNA, Rapid Isolation of Yeast DNA, Southern Blotting: Capillary Transfer of DNA to Membranes) or for construction of genomic libraries in bacteriophage {lambda} vectors. Approximately 200 µg of mammalian DNA, 100-150 kb in length, is obtained from 5 x 107 cultured aneuploid mammalian cells (e.g., HeLa cells). - [Read Isolation of High-molecular-weight DNA from Mammalian Cells Using Proteinase K and Phenol Protocol]

Isolation of Retroelement from Plant Genomic DNA - http://www.le.ac.uk/biology/phh4/retros.htm

Category: Plant Biology Protocols: Plant DNA RNA Isolation Protocols

Retroelements and their derivatives are a ubiquitous and abundant component of plant genomes. Major classes of retroelements include the Pseudoviridae (Ty1-copia ), the Metaviridae (Ty3 -gypsy) and the Retroposineae LINE (non-LTR) groups. All reverse transcribing elements can be included in a universal classification. Includes: Pseudoviridae (Ty1-copia) Degenerate Primers; Metaviridae (Ty3-gypsy) Element Degenerate Primers; LINE Element Degenerate Primers; PCR Programmes. - [Read Isolation of Retroelement from Plant Genomic DNA]

Isolation of Yeast Genomic DNA for Southern Blot Analysis Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4149

Category: Yeast Protocols: Yeast Nucleic Acid Protocols: Yeast DNA Isolation Protocols

Protocol (modified from Hoffman and Winston 1987) describes the purification of genomic DNA from yeast cells. - [Read Isolation of Yeast Genomic DNA for Southern Blot Analysis Protocol]

Ligation of Bacteriophage lambda Arms to Fragments of Foreign Genomic DNA Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3980

Category: Viral Manipulation Protocols: Phage Lamda Manipulation Protocols

Pilot ligations and packaging reactions are used to establish the amounts of fragmented genomic DNA and bacteriophage {lambda} arms that yield the maximum number of recombinants. Additional ligation and packaging reactions may then be set up to yield a comprehensive library of genomic DNA. - [Read Ligation of Bacteriophage lambda Arms to Fragments of Foreign Genomic DNA Protocol]

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Populational Analysis of Genomic DNA Protocol

A protocol on the populational Analysis of genomic DNA.

Genomic DNA Labeling of Microarrays Protocol

DNA microarrays are an ordered arrangement of DNA molecules complementary to genes of interest that are "spotted" by robotic equipment onto a glass slide substrate. The expression of genes in cells can be monitored with microarrays by preparing cDNA from the mRNA of cells of interest and measuring the hybridization to the microarray. This protocol describes the labeling of genomic DNA for use as a probe for hybridization to the cDNA spotted on the array.

Vector Design Protocol for Transgenic Mice Generation

The protocol gives general considerations for the design of targeting vectors for transgenic mice. The protocol shares tips in the design of knock-out and knock-in vectors and some of their strategies for producing homologously recombined embryonic stem cells.

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