User Panel

My Panel

Bookmark Science Articles

genomic Protocols

Search results for: genomic

Protocols » Search Results

Search Results Protocol Links Sort by: Hits | Alphabetical

A Concise Guide to cArray Hybridization Procedures - DNA Microarray Analysis - http://www.tigr.org/tdb/microarray/conciseguide.html

Category: DNA Microarray Protocols

Hegde et al., Array Fabrication, microarray Printing, Probe Preparation and Hybridization, RNA Extraction, RNA labeling, Data Collection, Normalization, and Analysis.Institute for Genomic Research, Rockville, MD - [Read A Concise Guide to cArray Hybridization Procedures - DNA Microarray Analysis]

aCGH Labeling Protocol - http://genomic.dfci.harvard.edu/aCGHLabelingChecklistv1_9.pdf

Category: Cytogenetics Protocols: Array CGH

aCGH Labeling Protocol. Harvard University. - [Read aCGH Labeling Protocol]

AFLP For Positional Cloning - http://www-ciwdpb.stanford.edu/publications/methods/aflp.html

Category: Plant Biology Protocols: Plant Genetics Protocols

AFLP was designed as a highly sensitive method for DNA fingerprinting to be used in a variety of fields. We are using this technology to generate DNA based markers for cloning genes involved in phototropic responses in higher plants that have only been identified genetically by mutant phenotype. Protocol includes: Generate polymorphic recombinant F2 (or F3) population; Isolate genomic DNA; Restriction of DNA; Ligation of adapters; Pre-amplification of template DNA; AFLP-PCR; etc. - [Read AFLP For Positional Cloning]

AFLP Method - http://haveylab.hort.wisc.edu/protocol/AFLP.html

Category: PCR Protocols: AFLP PCR

AFLP Procecure, includes restriction Digestion of Genomic DNA. Michael Havey - Vegetable Genetics Laboratory - [Read AFLP Method]

AFLP protocol - http://www.msu.edu/user/hazensam/aflp/AFLPprotocolMSU.html

Category: PCR Protocols: AFLP PCR

Restriction Digestion of Genomic DNA , Adapter preparation, Ligation of Adapters. Pre amplification Reactions. Gel electrophoresis, Silver Staining. AFLP Protocol and procedure. - [Read AFLP protocol]

AFLP: a new technique for DNA fingerprinting. - http://www.pubmedcentral.gov/articlerender.fcgi?tool=pubmed&pubmedid=7501463

Category: PCR Protocols: AFLP PCR

P Vos, R Hogers, M Bleeker, M Reijans, T van de Lee, M Hornes, A Frijters, J Pot, J Peleman, and M Kuiper. 1995. Nucleic Acid Research. The AFLP technique is based on the selective PCR amplification of restriction fragments from a total digest of genomic - [Read AFLP: a new technique for DNA fingerprinting.]

Amplification and Storage of a Cosmid Library: Amplification in Liquid Culture Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot4001

Category: DNA Protocols: Cosmid Library Protocols

Amplification of cosmid libraries may result in distorted representation of cloned genomic sequences and should be avoided wherever possible. If amplification should become necessary, it is best to expand the library by growth in liquid medium. - [Read Amplification and Storage of a Cosmid Library: Amplification in Liquid Culture Protocol]

Amplification and Storage of a Cosmid Library: Amplification on Filters Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot4002

Category: DNA Protocols: Cosmid Library Protocols

Amplification of cosmid libraries may result in distorted representation of cloned genomic sequences and should be avoided wherever possible. In this method of amplification, distortion of the library is rarely a problem because at no stage are bacteria containing different recombinant cosmids grown in competition with one another. - [Read Amplification and Storage of a Cosmid Library: Amplification on Filters Protocol]

An introduction to AFLP and fAFLP - http://ravel.zoology.wisc.edu/sgaap/AFLP_html/fAFLP_Introduction.htm

Category: PCR Protocols: AFLP PCR

An introduction to AFLP and fAFLP. Mark E. Berres, University of Wisconsin. Amplified fragment-length polymorphism (AFLP) or its fluorescent version (fAFLP) is a PCR-based fingerprinting technology. AFLP basically involves the restriction of genomic DNA - [Read An introduction to AFLP and fAFLP]

Analysis of DNA Fragmentation Using Agarose Gel Electrophoresis (Subscription Required) - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4429

Category: Cell Biology and Cell Culture: Apoptosis Protocols: Apoptosis Analysis

Analysis of DNA Fragmentation Using Agarose Gel Electrophoresis Shailaja Kasibhatla et al. This protocol provides a qualitative method for assessing cell death by detecting DNA fragments using agarose gel electrophoresis. One of the classic features of apoptosis is the cleavage of the genomic DNA into oligonucleosomal fragments represented by multiples of 180-200 bp. Visualizing these fragments can aid in characterizing an apoptotic event. May be combined with more quantitative methods. - [Read Analysis of DNA Fragmentation Using Agarose Gel Electrophoresis (Subscription Required)]

Analysis of DNA Methylation using Bisulphite Sequencing Protocol - http://www.methods.info/Methods/DNA_methylation/Bisulphite_sequencing.html

Category: PCR Protocols: Bisulfite Conversion Based PCR Protocols

Protocol for the analysis of DNA methylation using bisulphite sequencing. Method allows precise analysis of methylation in a certain region by converting all nonmethylated cytosines into tymines, while methylated cytosines remain unchanged. This method requires small amount of genomic DNA and therefore seems to be very useful for the analysis of clinical samples, where the material amount is limited.
- [Read Analysis of DNA Methylation using Bisulphite Sequencing Protocol]

Bacteriophage Lamda Plant Genomic Library Construction Protocol - http://www.oardc.ohio-state.edu/stockingerlab/Protocols/GenomicDNAPhageLibraryConstruction.pdf

Category: DNA Protocols: Genomic DNA Library Protocols

Protocol for bacteriophage lamda plant genomic library construction. Includes: Preparation of the insert DNA; Pilot Sau3A Digestion; Full-scale partial digestions. - [Read Bacteriophage Lamda Plant Genomic Library Construction Protocol]

C. elegans Genomic DNA Prep Protocol - http://www.k-state.edu/hermanlab/protocols/genomic_dna_prep.htm

Category: C. elegans Protocols: C. elegans Genetics Protocols

Protocol for C. elegans genomic DNA prep. - [Read C. elegans Genomic DNA Prep Protocol]

Calcium-phosphate-mediated Transfection of Cells with High-molecular-weight Genomic DNA - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3872

Category: Transfection Protocols: Calcium Phosphate Transfection Protocols

This protocol,is based on the venerable method of Graham and van der Eb (1973). The procedure is used chiefly to generate stable lines of cells carrying chromosomally integrated copies of the transfected DNA. - [Read Calcium-phosphate-mediated Transfection of Cells with High-molecular-weight Genomic DNA]

CGH Protocols - http://amba.charite.de/cgh/protocol/02/prot02.html

Category: Cytogenetics Protocols: Comparative Genomic Hybridization CGH Protocols

A nice map linking to protocols for CGH Comparative Genomic Hybridization. Hybridization protocols, detection and analysis. Institut fÃ¼r Pathologie University Hospital CharitÃ© - [Read CGH Protocols]

ChIP on ChIP Protocol PDF - http://genomecenter.ucdavis.edu/farnham/farnham/protocols/MOJO%20Dec%202003%20ChIP-chip%20protocol.pdf

Category: DNA Protocols: Chromatin Immunoprecipitation Protocols

Method to identify genomic targets of DNA-binding factors. Chromatin immunoprecipitation (ChIP) assay on high-throughput microarray based methods for discovering genomic regions occupied by human DNA-binding proteins. Oberley and Farnham. - [Read ChIP on ChIP Protocol PDF]

COMPARATIVE GENOMIC HYBRIDIZATION (CGH) PROTOCOL - http://users.ugent.be/~fspelema/neubla/protocol/cgh.htm

Category: Cytogenetics Protocols: Comparative Genomic Hybridization CGH Protocols

SAVING OF DAPI-IMAGES, SLIDE PRETREATMENT, PROBE PREPARATION, PROBE DETECTION, washing blocking detection, Counterstaining with DAPI. Institute of Pathology,Humboldt-University of Berlin - [Read COMPARATIVE GENOMIC HYBRIDIZATION (CGH) PROTOCOL]

Comparative Genomic Hybridization (CGH) Protocol - http://cgap-mf.nih.gov/Protocols/DNARNAProteomicAnalysis/DNA/CGH.html#Protocol

Category: Cytogenetics Protocols

Protocol for comparative genomic hybridization (CGH). Includes: Theory of CGH; Sensitivity; Applications of CGH. - [Read Comparative Genomic Hybridization (CGH) Protocol]

Comparative Genomic Hybridization PDF - http://www.utoronto.ca/cancyto/protocols_software/CGH_protocol.pdf

Category: Cytogenetics Protocols: Comparative Genomic Hybridization CGH Protocols

Excellent Detailed CGH Comparative Genomic Hybridization Protocol. Squire Lab, Univ. Toronto. Includes: Labeling, Gel Interpretation, Slide Pre-Treatment and Denaturation, Probe Denaturation, Hybridization and Post Hybridization Washes and Detection, Tips - [Read Comparative Genomic Hybridization PDF]

Construction of a Yeast Genomic Library Protocol - http://www.bio.com/protocolstools/protocol.jhtml?id=p361

Category: DNA Protocols: Genomic DNA Library Protocols

Protocol for the construction of a Yeast genomic library. Includes: Prepare the genomic DNA; Prepare the Library Vector; Ligate the Digested Genomic DNA to the Digested Vector DNA; Prepare Library DNA from Bacteria. - [Read Construction of a Yeast Genomic Library Protocol]

Construction of Genomic DNA Libraries in Cosmid Vectors Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot4000

Category: DNA Protocols: Cosmid Library Protocols

Protocol describes how to construct a library of 35-45-kb fragments of genomic DNA in the double cos site cosmid vector, SuperCos-1. The steps include: Linearization and dephosphorylation of SuperCos-1 DNA; Partial digestion of high-molecular-weight DNA with MboI; Dephosphorylation of high-molecular-weight genomic DNA; Ligation of cosmid arms to genomic DNA: Packaging and plating recombinants; Isolation and analysis of recombinant cosmids: Validation of the library. - [Read Construction of Genomic DNA Libraries in Cosmid Vectors Protocol]

Detection of Chromosomal Imbalances Using DOP-PCR and Comparative Genomic Hybridization (CGH) - http://www.roche-applied-science.com/PROD_INF/MANUALS/InSitu/pdf/ISH_95-103.pdf

Category: Cytogenetics Protocols

CGH provides a comprehensive picture of all chromosomal gains and losses within a single experiment. In contrast to banding analysis, CGH requires no preparation of metaphase chromosomes from the cells to be analyzed (which in certain tumor types may be difficult or even impossible). - [Read Detection of Chromosomal Imbalances Using DOP-PCR and Comparative Genomic Hybridization (CGH)]

Detection of Chromosomal Imbalances using DOP-PCR and Comparative Genomic Hybridization (CGH) - http://www.roche-applied-science.com/PROD_INF/MANUALS/InSitu/pdf/ISH_95-103.pdf

Category: Cytogenetics Protocols: Spectral Karyotyping SKY Protocols

Protocol for the Detection of chromosomal imbalances using DOP-PCR and comparative genomic hybridization (CGH). Includes: Preparation and labeling of DOP-PCR products; Comparative genomic hybridization (CGH). - [Read Detection of Chromosomal Imbalances using DOP-PCR and Comparative Genomic Hybridization (CGH)]

Direct Selection of cDNAs with Large Genomic DNA Clones Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot4077

Category: Genetics and Genomics: Genotyping Protocols: Mutation Detection Protocols

The goal of this method is to identify transcriptionally active genes in cloned segments of genomic DNA. The protocol uses hybridization and affinity purification to recover biotin-labeled cDNAs that bind to a 500-kb segment of human DNA cloned in a BAC vector. However, the method can be easily adapted to other clones of genomic DNAs cloned in high-capacity vectors. - [Read Direct Selection of cDNAs with Large Genomic DNA Clones Protocol]

Direct Sequencing Using MultiPlex PCR-Based Method - http://www.genome.ou.edu/MultiplexPCRbasedSeq.html

Category: DNA Protocols: DNA Sequencing Protocols

Direct sequencing from amplified bacterial and large insert cloned human or mouse genomic DNA via an improved MultiPlex PCR-based method. Includes: Preparing primers for MP-PCR; Amplification; PCR Product Clean-up; Sequencing. - [Read Direct Sequencing Using MultiPlex PCR-Based Method]

Articles (1-3 of 3)

Populational Analysis of Genomic DNA Protocol

A protocol on the populational Analysis of genomic DNA.

Genomic DNA Labeling of Microarrays Protocol

DNA microarrays are an ordered arrangement of DNA molecules complementary to genes of interest that are "spotted" by robotic equipment onto a glass slide substrate. The expression of genes in cells can be monitored with microarrays by preparing cDNA from the mRNA of cells of interest and measuring the hybridization to the microarray. This protocol describes the labeling of genomic DNA for use as a probe for hybridization to the cDNA spotted on the array.

Vector Design Protocol for Transgenic Mice Generation

The protocol gives general considerations for the design of targeting vectors for transgenic mice. The protocol shares tips in the design of knock-out and knock-in vectors and some of their strategies for producing homologously recombined embryonic stem cells.

[1-3]

Items 1-25 out of 80 displayed.

First [1] 2 3 4 Next Last

Total records: 80