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A Triple Color FISH Technique for Mouse Chromosome Identification - http://info.med.yale.edu/genetics/ward/tavi/mamgenome.html

Category: BACs Bacterial Artificial Chromosome Protocols

Describe a set of BACs that map close to the centromeric and distal chromosomal ends for each mouse chromosome. - [Read A Triple Color FISH Technique for Mouse Chromosome Identification]

AFLP Method - http://haveylab.hort.wisc.edu/protocol/AFLP.html

Category: PCR Protocols: AFLP PCR

AFLP Procecure, includes restriction Digestion of Genomic DNA. Michael Havey - Vegetable Genetics Laboratory - [Read AFLP Method]

Alexa Dyes, Cy Dyes and Other Fluors: Succinimidyl Esters - http://info.med.yale.edu/genetics/ward/tavi/FISHdyes.html

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Alexa Dyes, Cy Dyes and other Fluors: succinimidyl esters. - [Read Alexa Dyes, Cy Dyes and Other Fluors: Succinimidyl Esters]

Antibody Staining of Dissected Gonads Protocol - http://www.genetics.wustl.edu/tslab/Protocols/gonad_antibody.html

Category: C. elegans Protocols: C. elegans Staining Protocols

Protocol for antibody staining of dissected gonads. - [Read Antibody Staining of Dissected Gonads Protocol]

Antigen Retrieval Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/A2.html

Category: Immunohistochemistry Protocols: Antigen Retrieval Protocols

Protocol for antigen retrieval. - [Read Antigen Retrieval Protocol]

b-galactosidase Solution Assay Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/B2.html

Category: Immunohistochemistry Protocols

Protocol for b-galactosidase solution assay. - [Read b-galactosidase Solution Assay Protocol]

Bacteria-Mediated (Feeding) RNAi Protocol - http://www.genetics.wustl.edu/tslab/Protocols/Feeding_RNAi.htm

Category: C. elegans Protocols

Protocol for bacteria- mediated (feeding) RNAi. - [Read Bacteria-Mediated (Feeding) RNAi Protocol]

C. elegans Gonad Fixation Protocol - http://www.genetics.wustl.edu/tslab/Protocols/gonad_fixation.html

Category: C. elegans Protocols: C. elegans Fixing Protocols

Protocol for C.elegans gonad fixation. Includes fixation with: Methanol, Formaldehyde, Methanol / Formadehyde, Glutaraldehyde / Formaldehyde. - [Read C. elegans Gonad Fixation Protocol]

CHAPOL Lineage Analyis Protocol - http://genetics.med.harvard.edu/~cepko/protocol/CHAPOL.pdf

Category: Viral Culture and Isolation Protocols: Viral Culture Protocols

Protocol for CHAPOL lineage analysis. - [Read CHAPOL Lineage Analyis Protocol]

ChIP Protocol - http://www.genetics.ucla.edu/labs/fan/Protocols_ChIP.htm

Category: DNA Protocols: Chromatin Immunoprecipitation Protocols

ChIP Protocol. Fan Lab UCLA. - [Read ChIP Protocol]

Chromosomal DNA Preparation Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/C3.html

Category: Nucleic Acid Purification Protocols: DNA Isolation Protocols: DNA Isolation from Mammalian Cells Protocols

Protocol for chromosomal DNA preparation. Protocol was developed for cultured cells but should be appropriate for dissociated tissues as well. - [Read Chromosomal DNA Preparation Protocol]

Color Changing Karyotyping (CCK) Protocol: An M-FISH/SKY Alternative - http://info.med.yale.edu/genetics/ward/tavi/CCKmethod.html

Category: Cytogenetics Protocols

Protocol for color changing karytoping (CCK): an M-FISH/SKY alternative. Includes: Slide preparation and hybridization; Probes, dyes and antibodies; Probe detection and image capture; Image processing. - [Read Color Changing Karyotyping (CCK) Protocol: An M-FISH/SKY Alternative]

Conditioned Medium for Retinal Explant Culture Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/C6.html

Category: Neuroscience Protocols: Neuronal Cell Culture Protocols: Neuronal Cell Culture Media Protocols

Protocol for conditioned medium for retinal explant culture. - [Read Conditioned Medium for Retinal Explant Culture Protocol]

COS Cell Transfection Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/C5.html

Category: Transfection Protocols

COS cell transfection protocol. Includes solutions: DEAE Dextran and 1000X Chloroquine - [Read COS Cell Transfection Protocol]

Cryosectioning Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/C7.html

Category: Immunohistochemistry Protocols: Cryosectioning Protocols

Protocol for cryosectioning. While the timing of the various steps in this protocol are probably not critical, process the tissue all at once to ensure that RNA and/or proteins do not get degraded. Includes: 20% Paraformaldehyde/4% Paraformaldehyde-PBS; Sucrose/PBS. - [Read Cryosectioning Protocol]

Cryptic Translocation Identification in Human and Mouse Using Several Telomeric Multiplex FISH - http://info.med.yale.edu/genetics/ward/tavi/TMFISH.html

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Protocol for cryptic translocation identification in human and mouse using several telomeric multiplex FISH (TM-FISH) strategies. - [Read Cryptic Translocation Identification in Human and Mouse Using Several Telomeric Multiplex FISH]

CsCl Prep of Plasmid DNA Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/C1.html

Category: Nucleic Acid Purification Protocols: DNA Isolation Protocols: DNA Plasmid Preps Protocols

Protocol for CsCl prep of plasmid DNA. This is a standard large scale prep. For plasmid DNA which gives a yield of 0.5 1.0 mg. - [Read CsCl Prep of Plasmid DNA Protocol]

Custom Fluorescent Nucleotide Synthesis/Nucleic Acid Labeling Protocol - http://info.med.yale.edu/genetics/ward/tavi/n_coupling.html

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Protocol for custom fluorescent nucleotide synthesis/nucleic acid labeling. - [Read Custom Fluorescent Nucleotide Synthesis/Nucleic Acid Labeling Protocol]

Cytosolic Extract & Immunoprecipitation Protocol - http://www.genetics.wustl.edu/tslab/Protocols/CytosolicExtractv1_v2.htm

Category: C. elegans Protocols

Protocol for cytosolic extract & immunoprecipitation. Includes: Culture; Harvest; Extract; Immunoprecipitation. - [Read Cytosolic Extract & Immunoprecipitation Protocol]

DAPI Staining Protocol - http://www.genetics.wustl.edu/tslab/Protocols/dapi_staining.html

Category: C. elegans Protocols: C. elegans Staining Protocols

Protocol for DAPI staining. - [Read DAPI Staining Protocol]

Detection of ß-Galactosidase and Alkaline Phosphastase Activities in Tissue Protocol - http://genetics.med.harvard.edu/~cepko/protocol/xgalplap-stain.htm

Category: Neuroscience Protocols: Histology Stains Protocols: Enzyme Histochemical Stains Protocols

Protocol for detection of ß-Galactosidase and alkaline phosphastase activities in tissue. - [Read Detection of ß-Galactosidase and Alkaline Phosphastase Activities in Tissue Protocol]

Dissociated Retina Immunohistochemistry Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/D6.html

Category: Immunohistochemistry Protocols

Protocol for dissociated retina immunohistochemistry. - [Read Dissociated Retina Immunohistochemistry Protocol]

Dissociated Retinal Cell Scoring Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/score.html

Category: Immunohistochemistry Protocols

Protocol for dissociated retinal cell scoring. Includes: Rods; Cones; Bipolars; Müller Glial Cells; Pan-Amacrine/Horizontal. - [Read Dissociated Retinal Cell Scoring Protocol]

DNA Fragment Purification from Agarose or Acrylamide Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/D5.html

Category: Nucleic Acid Purification Protocols: DNA Isolation Protocols

Protocol for DNA fragment purification from agarose or acrylamide. For fragments from 200 bp to 10 kb the agarose purification is ideal. For smaller fragments (20 bp to 400 bp) the acrylamide purification is preferred. - [Read DNA Fragment Purification from Agarose or Acrylamide Protocol]

DNA Plasmid Prep. Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/D1.html

Category: Nucleic Acid Purification Protocols: DNA Isolation Protocols: DNA Plasmid Preps Protocols

Protocol for DNA plasmid prep. - [Read DNA Plasmid Prep. Protocol]

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