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Preparation of Bacteriophage lambda DNA Cleaved with a Single Restriction Enzyme Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3974

Category: Viral Manipulation Protocols: Phage Lamda Manipulation Protocols

Bacteriophage {lambda} vectors that allow genetic selection of recombinant bacteriophages (e.g., the EMBL series, {lambda}2001, {lambda}DASH, {lambda}ZAP, and {lambda}gt10) can be prepared for cloning by simple digestion by one or more restriction enzymes. - [Read Preparation of Bacteriophage lambda DNA Cleaved with a Single Restriction Enzyme Protocol]

Pristionchus Pacificus Genetic Protocols - http://www.wormbook.org/chapters/www_ppageneticprotocols/ppageneticprotocols.pdf

Category: Developmental Biology: Utilizing Model Organisms Protocols

Protocols on the genetics of Pristionchus pacificus. Includes: Freezing worms; Mutagenesis; Construction of deletion libraries to generate P. pacificus gene knock-outs; RNAi and morpholino by injection. - [Read Pristionchus Pacificus Genetic Protocols]

Pristionchus pacificus Genetic Protocols - http://www.wormbook.org/chapters/www_ppageneticprotocols/ppageneticprotocols.pdf

Category: Laboratory Model Organisms Protocols

Genetic Protocols for Pristionchus pacificus. Includes: Freezing worms; EMS mutagenesis; Psoralen mutagenesis; Construction of deletion libraries to generate P. pacificus gene knock-outs; Designing primers for the gene of interest; RNAi and morpholino by injection. - [Read Pristionchus pacificus Genetic Protocols]

RNAi Screens in C. elegans Protocol - http://www.natureprotocols.com/2006/07/19/rnai_screens_in_c_elegans_in_a.php

Category: RNAi Technology Protocols: RNAi C. elegans Protocols

Protocol for RNAi screens in C. elegans in a 96-well liquid format and their application to the systematic identification of genetic interactions. The procedure allows thousands of RNAi feeding experiments to be performed per investigator per day. - [Read RNAi Screens in C. elegans Protocol]

SNPs: Introduction and two-point mapping - http://www.wormbook.org/chapters/www_SNPsintrotwopointmap/SNPsintrotwopointmap.html

Category: DNA Protocols: SNP Protocols

Genetic mapping and manipulation: Chapter 4-SNPs: Introduction and two-point mapping. The SNP database and other information. wormbook.org - [Read SNPs: Introduction and two-point mapping]

Streaking Yeast Stocks Protocols - http://humgen.wustl.edu/hdk_lab_manual/yeast/yeast1.html

Category: Yeast Cell Culture Protocols: Yeast Media, Solutions and Stocks Protocols

Some yeast strains are unstable (e. g., small YAC-bearing strains) and need to be repurified by streaking on an agar plate and then verifying the genetic content of the isolated colony before proceeding. In cases where the strain is unstable, plan to streak the cells onto the selective medium to retain the desired stock, (however, most strains can be streaked onto the complete medium, YPD). - [Read Streaking Yeast Stocks Protocols]

Tetrad Dissection Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4181

Category: Yeast Cell Culture Protocols: Yeast Sporulation Protocols

This protocol describes dissection of yeast tetrads. Tetrad analysis is useful for linkage studies and for constructing strains for genetic and biochemical experiments. - [Read Tetrad Dissection Protocol]

Two-hybrid analysis of genetic regulatory networks - http://proteome.wayne.edu/YTHnetworks.html

Category: Molecular Model Organisms: Yeast Two-Hybrid Screen Protocol

Finley and Brent. Excellent Two-Hybrid Resource Article and Protocols. Very Detailed. - [Read Two-hybrid analysis of genetic regulatory networks]

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Picking Transfected ES Cell Clones Protocol

This protocol a protocol on how to generate transfected embryonic stem (ES) cell clones. The previous protocol in this series is the Protocol for Electroporation of ES cells. The next protocol in the series is the Protocol on Disaggregation, Expansion, and Freezing of Transfected ES Clones.

Vector Design Protocol for Transgenic Mice Generation

The protocol gives general considerations for the design of targeting vectors for transgenic mice. The protocol shares tips in the design of knock-out and knock-in vectors and some of their strategies for producing homologously recombined embryonic stem cells.

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