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Acrylamide Gels for Resolving Small (25bp-700bp) DNA Fragments Protocol - http://www.oardc.ohio-state.edu/stockingerlab/Protocols/AcrylamideGels%28DNA%29.pdf

Category: Nucleic Acid Electrophoresis Protocols: DNA Electrophoresis Protocols: DNA Electrophoresis in Polyacrylamide Gels Protocols

Protocol for acrylamide gels for resolving small (25bp-700bp) DNA fragments. - [Read Acrylamide Gels for Resolving Small (25bp-700bp) DNA Fragments Protocol]

Agarose Gel DNA Recovery Protocol - http://rothlab.ucdavis.edu/protocols/DNA-from-Agarose-Review.html

Category: DNA Protocols: DNA Extraction Protocols: Agarose Gel DNA Extraction Protocols

Agarose Gel DNA Recovery Protocol - Recovering DNA from agarose gels. Paul N. Hengen, Ph.D - [Read Agarose Gel DNA Recovery Protocol]

Agarose Gels Protocol - http://www.oardc.ohio-state.edu/stockingerlab/Protocols/AgaroseGels.pdf

Category: Nucleic Acid Electrophoresis Protocols

Protocol for agarose gel. - [Read Agarose Gels Protocol]

Alkaline Agarose Gel Electrophoresis Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot4027

Category: Nucleic Acid Electrophoresis Protocols: DNA Electrophoresis Protocols: DNA Electrophoresis in Agarose Gels Protocols

Alkaline agarose gels are used chiefly to measure the size of first and second strands of cDNA (Construction of cDNA Libraries Stage 1: Synthesis of First-strand cDNA Catalyzed by Reverse Transcriptase) and to analyze the size of the DNA strand after digestion of DNA-RNA hybrids with nucleases such as S1. - [Read Alkaline Agarose Gel Electrophoresis Protocol]

Analysis of Protein Gels (SDS-PAGE) - http://www.ruf.rice.edu/~bioslabs/studies/sds-page/gellab3.html

Category: Protein Protocols: Protein Electrophoresis

An excellent guide on the analysis of proteins on SDS-PAGE gels, through staining with coomassie blue dye and western blot analysis. Analysis of Protein Gels (SDS-PAGE). David R. Caprette, Rice University. - [Read Analysis of Protein Gels (SDS-PAGE)]

Application of Direct Current Electric Fields to Cells and Tissues in vitro - http://www.natureprotocols.com/2006/12/12/application_of_direct_current.php

Category: Cell Biology and Cell Culture

Protocol applies EFs to cells in vitro but has been modified and to use electrotactic chambers to accommodate cells growing in planar culture or in three-dimensional (3D) gels, en bloc tissue cultures in 3D and possible small embryos, such as that from frog and zebra fish. The EF is applied to the cells or tissues cultured in a customer designed electrotactic chamber via agar salt bridges, Steinbergâ€™s solution and Ag/AgCl electrodes. - [Read Application of Direct Current Electric Fields to Cells and Tissues in vitro]

Autoradiography and Reading of Sequencing Gels Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3588

Category: DNA Protocols: DNA Sequencing Protocols

Protocol describes fixation of a sequencing gel and processingin preparation for autoradiography. - [Read Autoradiography and Reading of Sequencing Gels Protocol]

Autoradiography Protocols - http://pingu.salk.edu/~sefton/Hyper_protocols/ARG.html

Category: Radioactivity: Autoradiography

Autoradiography Protocols. Exposing gels and plates containing radioactive samples to X-ray film. Detection of 3H. Detection of 35S and 14C. Detection of 32P and 125I. Bart's Cookbook. - [Read Autoradiography Protocols]

Detection of DNA in Agarose Gels Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot4022

Category: Nucleic Acid Electrophoresis Protocols: DNA Electrophoresis Protocols: DNA Electrophoresis in Agarose Gels Protocols

The most convenient and commonly used method to visualize DNA in agarose gels is staining with the fluorescent dye ethidium bromide. Ethidium bromide can be used to detect both singleand double-stranded nucleic acids (both DNA and RNA). However, the affinity of the dye for single-stranded nucleic acid is relatively low and the fluorescent yield is comparatively poor. - [Read Detection of DNA in Agarose Gels Protocol]

Detection of DNA in Polyacrylamide Gels by Autoradiography Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot4029

Category: Nucleic Acid Electrophoresis Protocols: DNA Electrophoresis Protocols: DNA Electrophoresis in Polyacrylamide Gels Protocols

Detection of radioactive DNA in polyacrylamide gels by autoradiography. - [Read Detection of DNA in Polyacrylamide Gels by Autoradiography Protocol]

Detection of DNA in Polyacrylamide Gels by Staining Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3718

Category: Nucleic Acid Electrophoresis Protocols: DNA Electrophoresis Protocols: DNA Electrophoresis in Polyacrylamide Gels Protocols

Staining bands of DNA in polyacrylamide gels with ethidium bromide, SYBR-Gold, and methylene blue. - [Read Detection of DNA in Polyacrylamide Gels by Staining Protocol]

Direct Retrieval of DNA Fragments from Pulsed-field Gels Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot4083

Category: Nucleic Acid Electrophoresis Protocols: Pulsed Field Gel Electrophoresis Protocols

Protocol for direct retrieval of DNA fragments from pulsed-field gels. A gel slice containing a fragment of DNA resolved by pulsed-field gel electrophoresis is treated with agarase. The released DNA can be used as a substrate for ligation or restriction without further purification. - [Read Direct Retrieval of DNA Fragments from Pulsed-field Gels Protocol]

DNA Extraction from Agarose Gels Protocol - http://www.methodbook.net/dna/gelextrc.html

Category: DNA Protocols: DNA Extraction Protocols: Agarose Gel DNA Extraction Protocols

DNA Extraction from Agarose Gels Protocol. The page includes cutting out the DNA band from the gel, and describes three methods including 1) Spin-columns (Nucleic acid purification columns), 2) using Dialysis tubing (semi-permeable membrane, Visking tubing), and the 3) Paper strip method.Matt Lewis, Department of Pathology, University of Liverpool. - [Read DNA Extraction from Agarose Gels Protocol]

Electrophoresis - Agarose Gels for Single Stranded DNA Protocol - http://rothlab.ucdavis.edu/protocols/E02.html

Category: Nucleic Acid Electrophoresis Protocols: DNA Electrophoresis Protocols: DNA Electrophoresis in Agarose Gels Protocols

Protocol for electrophoresis of single stranded DNA using agarose gel. - [Read Electrophoresis - Agarose Gels for Single Stranded DNA Protocol]

Electrophoresis of DNA in Agarose Gels. - http://omrf.ouhsc.edu/~frank/Agarose.html

Category: Molecular Biology Protocols: Agarose Gel Preparation

Electrophoresis of DNA in Agarose Gels. Frank Lab. - [Read Electrophoresis of DNA in Agarose Gels.]

ENZYME-ASSISTED IMMUNOELECTROBLOTTING (IEB OR WESTERN BLOTTING) - http://www.mcb.uct.ac.za/western.htm

Category: Protein Protocols: Western Blot Protocol

ENZYME-ASSISTED IMMUNOELECTROBLOTTING (IEB OR WESTERN BLOTTING. E.P. Rybicki and Maud Purves. Department of Microbiology. Copper Staining of Gels, Indirect enzyme immunoassay, Staining Proteins on Membranes, Blotting Buffer, Staining of proteins in gels - [Read ENZYME-ASSISTED IMMUNOELECTROBLOTTING (IEB OR WESTERN BLOTTING)]

Gel Filtration Column Chromatography - http://www4.nau.edu/biology/bio349l/Documents/bio349l/Protocols_for_week_3.pdf

Category: Chromatography Protocols: Gel Filtration Chromatography Protocols

Gel Filtration Column Chromatography Protocol. Includes information on: Calibrating the column, Applying standards to the column, Running SDS-PAGE Gels (Tris-Tricine â€œSchÃ¤ggerâ€ Gels) and includes questions. North Arizona University. - [Read Gel Filtration Column Chromatography]

Immunoblotting: Preparing Protein Solutions Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/21/pdb.prot4298

Category: Western Blot Protocols: General Western Blot Methods Protocols

Protocol describes how proteins are mixed with a sample buffer and prepared for electrophoresis on standard Tris/glycine SDS-polyacrylamide gels. - [Read Immunoblotting: Preparing Protein Solutions Protocol]

Immunoblotting: Semi-Dry Electrophoretic Transfer of Proteins from Gels to Membranes Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/16/pdb.prot4301

Category: Western Blot Protocols: General Western Blot Methods Protocols

The transfer of proteins from a Tris/glycine SDS-polyacrylamide gel to a membrane using a semi-dry method is achieved by placing the gel next to a piece of nitrocellulose filter. This sandwich is placed directly between two plate electrodes, and the proteins are then transferred from the gel onto the filter. - [Read Immunoblotting: Semi-Dry Electrophoretic Transfer of Proteins from Gels to Membranes Protocol]

Immunoblotting: Submerged Electrophoretic Transfer of Proteins from Gels to Membranes Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/16/pdb.prot4302

Category: Western Blot Protocols: General Western Blot Methods Protocols

Transfer of proteins from a Tris/glycine SDS-polyacrylamide gel to a membrane using the submerged method is achieved by placing the gel next to a piece of nitrocellulose filter, submerging this sandwich in a large volume of transfer buffer in a transfer tank, and running current from one side of the transfer tank to another. The proteins are then eluted by transferring them from the gel onto the filter. - [Read Immunoblotting: Submerged Electrophoretic Transfer of Proteins from Gels to Membranes Protocol]

In Gel Digestion Protocols for Silver Stained and Coomassie Stained Gels - http://www.proteomics.com.cn/2005/papers/InGel.html

Category: Proteomic Protocols

In Gel Digestion Protocols for Silver Stained and Coomassie Stained Gels. (EMBL protocol). - [Read In Gel Digestion Protocols for Silver Stained and Coomassie Stained Gels]

In-gel digestion of proteins to be analyzed by mass spectrometry - http://www.narrador.embl-heidelberg.de/GroupPages/PageLink/activities/protocols/ingeldigest.html

Category: Proteomic Protocols

In-Gel Digestion Protocol. Excision of protein bands (spots) from polyacrylamide gels Reduction and alkylation . Reduction and alkylation . MALDI analysis of the supernatant after in-gel digestion. Extraction of Peptides.Matthias Wilm EMBL Bioanalytical R - [Read In-gel digestion of proteins to be analyzed by mass spectrometry]

Isolation of DNA Fragments from Polyacrylamide Gels by the Crush and Soak Method Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot2936

Category: Nucleic Acid Electrophoresis Protocols: DNA Electrophoresis Protocols: DNA Electrophoresis in Polyacrylamide Gels Protocols

The "crush and soak" method, which works best for DNAs <1 kb in size, can be used to recover both singleand double-stranded DNAs from polyacrylamide gels. The yield of eluted DNA varies from <30% to >90% depending on the size of the DNA fragment. - [Read Isolation of DNA Fragments from Polyacrylamide Gels by the Crush and Soak Method Protocol]

Loading and Running DNA Sequencing Gels Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3794

Category: DNA Protocols: DNA Sequencing Protocols

Protocol for loading and running DNA sequencing gels. - [Read Loading and Running DNA Sequencing Gels Protocol]

Mass spectrometry compatible silver staining of gels protocol - http://www.narrador.embl-heidelberg.de/GroupPages/PageLink/activities/protocols/silverstain.html

Category: Mass Spectrometry Protocols

Mass spectrometry compatible silver staining of gels protocol. EMBL Bioanalytical Research Group. Wilm. - [Read Mass spectrometry compatible silver staining of gels protocol]

Articles (1-4 of 4)

Single Stranded Plasmid DNA Isolation Protocol

A Single Stranded Plasmid DNA Isolation Protocol describing the production and isolation of single-stranded DNA (ssDNA) using bacteriophagemid-containing bacteria and helper phage. Infection of the host cells with helper phage allows for packaging of ssDNA into bacteriophage. The ssDNA can then be isolated from phage particles.

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