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A Safe Method of Extracting DNA from Coccidioides immitis Protocol - http://www.fgsc.net/fgn42/burt.html

Category: Fungi Protocols: Fungi Genetics Protocols

Protocol describes a safe and convenient method of extracting DNA from Coccidioides immitis fungi in which the culture is killed by steaming, allowing removal from the containment facilities, as soon as possible. The method was first developed with the non-pathogen Neurospora crassa, has worked well for both C. immitis and H. capsulatum, and should be useful for extracting DNA from any pathogenic fungus. - [Read A Safe Method of Extracting DNA from Coccidioides immitis Protocol]

Detection of Fungi by Fluorescence Microscopy Using Fluorescent Brighteners - http://www.aspergillus.org.uk/indexhome.htm?secure/laboratory_protocols/./flbr.html~main

Category: Microscopy Protocols: Fluorescence Microscopy Protocols

Certain fluorescent dyes such as Blankophor have a high affinity for the b -glycosidically linked polysaccharides such as glucan and chitin, which are main the constituents of the fungal cell wall. Therefore, these fluorescent dyes can be used for screening clinical samples for the presence of fungal elements. This procedure can be performed using the following specimens: Nail, Skin, Bronchial alveolar lavage fluid, Sputum and Biopsies. - [Read Detection of Fungi by Fluorescence Microscopy Using Fluorescent Brighteners]

Detection of Fungi by Fluorescence Microscopy Using Fluorescent Brighteners Protocol - http://www.aspergillus.org.uk/indexhome.htm?secure/laboratory_protocols/./flbr.html~main

Category: Fungi Protocols

Protocol for the detection of fungi by fluorescence microscopy using fluorescent brighteners. - [Read Detection of Fungi by Fluorescence Microscopy Using Fluorescent Brighteners Protocol]

Enrichment for Microsatellite Sequences in Onion (Allium cepa L.) Protocol - http://barley.ipk-gatersleben.de/~dirkf/netscape.html

Category: Plant Biology Protocols: Plant Genetics Protocols

Microsatellite markers, also referred to as STMS (SequenceTagged Microsatellite Sites) or STR (Short Tandem Repeats) are widely used as molecular markers for intraspecific genotyping, molecular mapping and breeding purposes. The method described is an efficient,fast and relatively inexpensive way to obtain microsatellite markers without post-cloning selection methods. So far, the method has been successful in onion (Allium cepa L.), a plant with a large genome and for pathogenic fungi. - [Read Enrichment for Microsatellite Sequences in Onion (Allium cepa L.) Protocol]

Stain for Fungi Protocol - http://www.nottingham.ac.uk/pathology/protocols/grocott.html

Category: Neuroscience Protocols: Histology Stains Protocols

Protocol stain for Fungi: Methenamine Silver. - [Read Stain for Fungi Protocol]