fungal Protocols

Search results for: fungal

Protocols » Search Results

Search Results Protocol Links Sort by: Hits | Alphabetical

Assessing and Controlling Microbial Contamination in Cell Cultures Protocol - https://catalog.invitrogen.com/index.cfm?fuseaction=iProtocol.unitSectionTree&treeNodeID=9E662BAD9F445C2107BC142E6B0F41B0&objectid=6673A7AEBD864E91AC365EE3D108411A

Category: Cell Culture Protocols: Cell Culture Contamination Prevention and Removal Protocols

This unit describes how to detect and recognize bacterial, fungal, or mycoplasma contamination and how to verify the presence of mycoplasma. Strategies are described for dealing with culture contamination, if necessary. - [Read Assessing and Controlling Microbial Contamination in Cell Cultures Protocol]

Detection of Fungi by Fluorescence Microscopy Using Fluorescent Brighteners - http://www.aspergillus.org.uk/indexhome.htm?secure/laboratory_protocols/./flbr.html~main

Category: Microscopy Protocols: Fluorescence Microscopy Protocols

Certain fluorescent dyes such as Blankophor have a high affinity for the b -glycosidically linked polysaccharides such as glucan and chitin, which are main the constituents of the fungal cell wall. Therefore, these fluorescent dyes can be used for screening clinical samples for the presence of fungal elements. This procedure can be performed using the following specimens: Nail, Skin, Bronchial alveolar lavage fluid, Sputum and Biopsies. - [Read Detection of Fungi by Fluorescence Microscopy Using Fluorescent Brighteners]

Determination of Tissue Fungal Burden Utilizing Quantitative Real Time Polymerase Chain Reaction - http://www.sacmm.org/pdf/Determination%20of%20Tissue%20Fungal%20Burden%20utilizing%20Quantitative%20Real%20Time%20PCR.pdf

Category: Fungi Protocols: Fungi Genetics Protocols

Standard operating procedure for the determination of tissue fungal burden utilizing quantitative real time polymerase chain reaction (QPCR). This standard operating procedure will provide information on how to assess fungal tissue burden of infected animals by use of a single copy (FKS) or multicopy gene (18s RNA) to assess the number of fungal cell nuclei present. - [Read Determination of Tissue Fungal Burden Utilizing Quantitative Real Time Polymerase Chain Reaction]

Expression of Recombinant Aequorin as an Intracellular Calcium Reporter PDF - http://www.biology.ed.ac.uk/research/institutes/plant/PDF/2001/Shaw-2001-207.pdf

Category: Signalling Signal Transduction Protocols: Calcium Measurement Protocols

Shaw et al. Fungal Genetics and Biology 2001. first report of a plant pathogenic fungus for which aequorin-based Ca21 measurement protocols have been developed. - [Read Expression of Recombinant Aequorin as an Intracellular Calcium Reporter PDF]

Fungal DNA Isolation Method - http://wheat.pw.usda.gov/~lazo/methods/lazo/dnafun.html

Category: DNA Protocols: DNA Extraction Protocols

DNA isolation from various fungal species including: Cochliobolus, Aternaria, and Fusarium. Key steps: (1) the use of young lyophilized mycelial mats - yield less contaminating carbohydrates; (2) proteinase K in the extraction buffer to destroy DNases (f - [Read Fungal DNA Isolation Method]

Fungal DNA Isolation Protocol - http://wheat.pw.usda.gov/~lazo/methods/lazo/dnafun.html

Category: Nucleic Acid Purification Protocols: DNA Isolation Protocols

Protocol for fungal DNA isolation. The key elements in this prep are (1) the use of young lyophilized mycelial mats....young mats (4 days growth for C. carbonum)...yield less contaminating carbohydrates and other misc. junk (2) lots of proteinase K in the extraction buffer to kill Dnases (final =0.3mg/ml). - [Read Fungal DNA Isolation Protocol]

How to Deposit Materials in the Fungal Genetics Stock Center (FGSC) - http://www.fgsc.net/neurosporaprotocols/How%20to%20deposit%20materials%20final.pdf

Category: Fungi Protocols: Neurospora Protocols

Information and tips on how to deposit materials in the Fungal Genetics Stock Center (FGSC). - [Read How to Deposit Materials in the Fungal Genetics Stock Center (FGSC)]