fugene Protocols

Category: Transfection Protocols: Stable Transfection Protocols

The AfCS is utilizing antisense technology to manipulate signaling protein expression in the RAW 264.7 macrophage-like cell line. This can be achieved by the transfection of gene-specific antisense oligonucleotides (ASOs). The following procedure involves the transfection of ASOs into RAW 264.7 cells using FuGENE 6 transfection reagent. Subsequently, the isolated total RNA or protein from these transfected cells can be used to assess the level of mRNA or protein knockdown, respectively. - [Read Antisense Oligonucleotide Transfection of RAW 264.7 Cells with FuGENE 6 in a 24-Well Dish]

Category: Reporter Gene Assays: GFP Assay Protocols

The following protocol can be used for the development of stable cell lines expressing GFP fusion proteins. Although optimal transfection procedures (e.g., calcium phosphate, electroporation, or FuGENE 6 [Roche Applied Science]) vary depending on cell type, this general transfection procedure has been successful for stable transfection of HeLa, A-431, U2OS, BHK, and HT1080 cells. - [Read Constructing and Expressing GFP Fusion Proteins]

Category: Cell Biology and Cell Culture: Cell Transfection Protocol

FuGene Transfection. They use 4ul, we found we could use as little as 1.5 ul of Fugene-6. Lamond Lab. - [Read FuGene Transfection]

Category: Cell Biology and Cell Culture: Cell Transfection Protocol

Fugene Transfection Protocol PDF. Meyer Lab Standford. - [Read Fugene Transfection Protocol PDF]

Category: Cell Biology and Cell Culture: Cell Transfection Protocol

TRANSFECTION WITH FUGENE 6 Protocol and method. Johan Peränen Biocenter Helsinki. - [Read FuGene-6 Transfection Protocol PDF]