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Immunofluorescent Staining for the Laser Microdissection of Individual Cells Protocol - http://www.natureprotocols.com/2006/08/03/immunofluorescent_staining_for.php

Category: Primary Cell Isolation and Culture Protocols: Laser Capture Microdissection Protocols

Useful techniques to circumvent disruption of tissue structure in the analysis of gene expression are LCM and LDM. While they require specialized microscopes and systems, they are similar in that freshly-cut frozen tissue sections can be microdissected using either a general histological stain (like H&E) or by staining with fluorescently conjugated antibodies. The LCM system by Arcturus involves... - [Read Immunofluorescent Staining for the Laser Microdissection of Individual Cells Protocol]

Immunohistochemistry Enzyme HRP Staining Protocol - http://www.ihcworld.com/_protocols/general_IHC/immunoenzyme_pod.htm

Category: Immunohistochemistry Protocols

Protocol for immunohistochemistry enzyme HRP staining. Includes: Cell Linee; Frozen Sections; Paraffin Sections; Pretreatments of Tissue Sections. - [Read Immunohistochemistry Enzyme HRP Staining Protocol]

Immunohistochemistry using Anti-Ganglioside Antibodies - http://www.glycotech.com/protocols/Proto2.html

Category: Molecular Biology Protocols: Carbohydrate Protocols

Indirect method measuring immunofluorescence coupled to second antibody. Best for membrane antigens in addition to intra- and extracellular antigens, may be applied to frozen tissue sections, to cells in suspension, and to cells attached to glass slides or coverslips. Tadashi Tai~Head, Department of Tumor Immunology, The Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan - [Read Immunohistochemistry using Anti-Ganglioside Antibodies]

In Situ Cell Death (Apoptosis) Detection by TUNEL labeling, Protocol for Frozen Sections. - http://www.emory.edu/WILCOX/tunel.frzn.html

Category: Cell Biology and Cell Culture: Apoptosis Protocols: TUNEL assay apoptosis

In Situ Cell Death (Apoptosis) Detection by TUNEL labeling, Protocol for Frozen Sections. Wilcox et al. Modified. - [Read In Situ Cell Death (Apoptosis) Detection by TUNEL labeling, Protocol for Frozen Sections.]

In Situ Hybridization to Tissue Sections Protocol - http://cmgm.stanford.edu/~vesicle/protocols/in_situ.html

Category: Cytogenetics Protocols: In Situ Hybridization Tissue Sections Protocols

Protocol for in situ hydbridization to tissue sections. Includes: Paraffin Sections; Frozen Sections; Probe preparation; Hybridization and washes; Autoradiography. - [Read In Situ Hybridization to Tissue Sections Protocol]

Laser Capture Microdissection Protocols - http://lcm.path.uab.edu/protocols.htm

Category: Histology Protocols: Laser Capture and Microdissection

Preparation of Frozen Tissue for LCM, Preparing Sections from Frozen Tissue for Laser Microdissection, Preparing Sections from Fixed, Paraffin-embedded Tissue for Laser Microdissection Protocols and methods. Laser Microdissection Laboratory. UAB. - [Read Laser Capture Microdissection Protocols]

Long Term Lambda Phage Storage Protocol - http://humgen.wustl.edu/hdk_lab_manual/lambda/lambda6.html

Category: Cell Culture Protocols: Cell Storing and Cell Freezing Protocols

No special treatment is required to prepare a lysate for the active collection. The following procedure should be used for long-term storage of lambda clones in the archival collections. The phage are diluted in media containing 7% DMSO and frozen at -80 degrees C. - [Read Long Term Lambda Phage Storage Protocol]

Lymphoblastoid Cell Lines from Frozen Whole Blood - http://humgen.wustl.edu/hdk_lab_manual/hcc/hcc10.html

Category: Immunology: Macrophage Monocyte Protocols

Lymphoblastoid Cell Lines from Frozen Whole Blood. Rosalie Veile. Keller Lab. - [Read Lymphoblastoid Cell Lines from Frozen Whole Blood]

Peptide Handling and Storage - http://www.sigmaaldrich.com/Brands/Sigma_Genosys/Custom_Peptides/Key_Resources/Handling___Storage.html

Category: Peptide Protocols: Peptide Storage

In general, peptide solutions are stable for up to a week at 4Â°C. However, if the peptide sequence has inherent instability (Peptide Stability), it might be better to freeze the solution when not in use. Peptide solutions at pH>8 should also be frozen when not in use. Sigma Aldrich. - [Read Peptide Handling and Storage]

Performing a Hunt by Interaction Mating Protocol - http://proteome.wayne.edu/Matinghunt.html

Category: Yeast Protocols: Yeast Genetics Protocols

When more than one bait will be used to screen a single library, significant time and resources can be saved by performing the interactor hunt by interaction mating. In this protocol one strain is transformed with library DNA and the transformants are collected and frozen in aliquots. - [Read Performing a Hunt by Interaction Mating Protocol]

Preparation of Frozen Stocks of RAW 264.7 Cells - http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi/afcs_PP00000180.pdf?pid=PP00000180

Category: Cell Culture Protocols: Cell Lines Protocols

Protocol for the preparation of frozen stocks of RAW 264.7 cells. Includes: Freezing procedure and reagents. - [Read Preparation of Frozen Stocks of RAW 264.7 Cells]

Preparation of Paraffin Sections and Frozen Tissue for FISH Protocol - http://www.riedlab.nci.nih.gov/publications/2412.2845_Paraff_FrozTiss_Slide_Pretreat.pdf

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Protocol for the preparation of paraffin sections and frozen tissue for FISH. - [Read Preparation of Paraffin Sections and Frozen Tissue for FISH Protocol]

Preparation, LCM and RNA/DNA extraction of Frozen Tissue Sections Protocol - http://dir.nichd.nih.gov/lcm/LCMTAP.htm#I.%20Preparation,%20LCM%20and%20RNA/DNA%20extraction%20of%20Frozen%20Tissue%20Sections

Category: Primary Cell Isolation and Culture Protocols: Laser Capture Microdissection Protocols

Protocol for the preparation, LCM and RNA/DNA extraction of frozen tissue sections. Includes: Embedding, Cutting and Staining. - [Read Preparation, LCM and RNA/DNA extraction of Frozen Tissue Sections Protocol]

Preparing and Transforming Spheroplasts of Neurospora crassa Protocol - http://www.fgsc.net/neurosporaprotocols/How%20to%20Prepare%20Spheroplasts.pdf

Category: Fungi Protocols: Neurospora Protocols

Procedure for preparing and transforming spheroplasts of Neurospora crassa. The procedure is designed to produce a large number of spheroplasts to be stored frozen at -80°. - [Read Preparing and Transforming Spheroplasts of Neurospora crassa Protocol]

Preparing Frozen Tissue Sections for Immunostaining Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4328

Category: Immunohistochemistry Protocols: Antibody Staining Protocols

Frozen tissue sections show good preservation of tissue structure and antigens. The principle disadvantages of using them in immunostaining are that the specimens must be stored frozen, and a special microtome, known as a cryostat, is required. Also, many clinical specimens are not available in this form, and most classic histological descriptions of tissue structure and pathology are based on the use of paraffin-embedded sections of formalin-fixed material. - [Read Preparing Frozen Tissue Sections for Immunostaining Protocol]

Protein isolation with tissue array construction - http://147.52.72.117/ijmm/2003/volume12/number3/299.pdf

Category: Protein Protocols: Protein Extraction From Tissue

Combination of nucleic acid and protein isolation with tissue array construction: Using defined histologic regions in single frozen tissue blocks for multiple research purposes - [Read Protein isolation with tissue array construction]

Protocols for LCM Preparation and Analysis - http://dir.nichd.nih.gov/lcm/LCMTAP.htm

Category: Primary Cell Isolation and Culture Protocols: Laser Capture Microdissection Protocols

Protocols for LCM preparation and analysis. Includes protocols: Preparation, LCM and RNA/DNA extraction of Frozen Tissue Sections; Preparation and LCM of Paraffin Embedded Tissue Sections; Standard Protocols for Microdissected Tissue Analysis. - [Read Protocols for LCM Preparation and Analysis]

Reactivating Cell Lines and Cell Growth for DNA Preparation - http://humgen.wustl.edu/hdk_lab_manual/hcc/hcc4.html

Category: Cell Biology and Cell Culture: Cell Culture Techniques: Cell Line Preservation Freezing

Cell lines are reactivated and grown to a count of 1 x 108 cells. The cells are pelleted and stored frozen at -80 degrees C prior to DNA extraction. Donis-Keller. - [Read Reactivating Cell Lines and Cell Growth for DNA Preparation]

X-Gal Staining of Drosophila Frozen Cryostat Sections Protocol - http://vosshall.rockefeller.edu/protocols/X-galstainingprotocol.pdf

Category: Drosophila Protocols: Drosophila Staining Protocols

Protocol for X-gal staining of Drosophila frozen cryostat sections. - [Read X-Gal Staining of Drosophila Frozen Cryostat Sections Protocol]

Articles (1-2 of 2)

Immobilized Antigen Phage Antibody Selection Protocol

A protocol for the selection of Phage Antibodies using Immobilized Antigen. This method describes the selection of antibodies from bacteriophage antibody libraries that recognize a specific antigen. The phage display library of antibody-displaying phage particles is exposed to antigen attached to a solid substrate (Nunc Immuno™ tubes). The phage particles with affinity for antigen bind to the immobilized antigen and are selected from the library of phage expressing antibodies.