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Benton Davis Blots for cDNA Libraries - http://omrf.ouhsc.edu/~frank/BENTNDAV.html

Category: RNA Protocols: cDNA Libraries Library

Includes procedures for cDNA screens using secondary and tertiary screening. Dr. Bart Frank Lab. - [Read Benton Davis Blots for cDNA Libraries]

CALCIUM PHOSPATE TRANSFECTION OF HUMAN CELLS - http://omrf.ouhsc.edu/~frank/CAPO4TRANSFECT.html

Category: Cell Biology and Cell Culture: Cell Transfection Protocol

Simple Transfection using Calcium chloride and phosphate.Dr. Frank. Arthritis and Immunology Program, Oklahoma City. Medical Research Foundation. - [Read CALCIUM PHOSPATE TRANSFECTION OF HUMAN CELLS]

cDNA Synthesis and Cloning - http://omrf.ouhsc.edu/~frank/CDNA.html

Category: RNA Protocols: cDNA Libraries Library

Protocol for cDNA synthesis and cloning cDNA into plasmid vector. 1st Strand cDNA Synthesis, and determine the efficiency of first strand cDNA synthesis. Also includes second Strand cDNA Synthesis. Dr.Frank - [Read cDNA Synthesis and Cloning]

Detection of Recombinant Proteins by ELISA - http://omrf.ouhsc.edu/~frank/ELISA.html

Category: Recombinant Protein Protocols

ELISA detection of preparation of bacterially expressed protein. ELISA REAGENT RECIPES. Frank Lab. - [Read Detection of Recombinant Proteins by ELISA]

Detection of Recombinant Proteins by ELISA assay - http://omrf.ouhsc.edu/~frank/ELISA.html

Category: ELISA Protocols

Detection of Recombinant Proteins by ELISA protocol. Dr. Frank, Oklahoma Medical Research Foundation. - [Read Detection of Recombinant Proteins by ELISA assay]

DNA Microarray Procedures - http://omrf.ouhsc.edu/~frank/marraytraining.html

Category: DNA Microarray Protocols

Protocols, Frequently Asked Questions, Validation. Dr. Bart Frank - [Read DNA Microarray Procedures]

Electrophoresis of DNA in Agarose Gels. - http://omrf.ouhsc.edu/~frank/Agarose.html

Category: Molecular Biology Protocols: Agarose Gel Preparation

Electrophoresis of DNA in Agarose Gels. Frank Lab. - [Read Electrophoresis of DNA in Agarose Gels.]

Electroporation of Eukaryotic Cells - http://omrf.ouhsc.edu/~frank/EPOREUK.html

Category: Cell Biology and Cell Culture: Cell Electroporation Protocols

The protocol uses a Bio-Rad Gene Pulser II apparatus to electroporate DNA into human cell lines. Dr. Frank, Arthritis and Immunology Program, Oklahoma City Medical Research Foundation - [Read Electroporation of Eukaryotic Cells]

Freezing and Thawing Eukaryotic Cells - http://omrf.ouhsc.edu/~frank/EukFreeze.html

Category: Cell Biology and Cell Culture: Cell Culture Techniques: Cell Line Preservation Freezing

Freezing and Thawing Eukaryotic Cells in liquid nitrogen, Freezing Cell Lysates for Western Blots, Thawing Cells from Liquid Nitrogen. Dr. Bart Frank Oklahoma. - [Read Freezing and Thawing Eukaryotic Cells]

Immunoscreening of Lambda-GT11 Bacteriophage Libraries with Antibody Probes - http://omrf.ouhsc.edu/~frank/GT11.html

Category: RNA Protocols: cDNA Libraries Library

Adsroption of Anti-E. coli Antibodies, cDNA Library Screening, Frank Lab. Oklahoma Medical Research Foundation - [Read Immunoscreening of Lambda-GT11 Bacteriophage Libraries with Antibody Probes]

Insect Cell Culture and Baculovirus Infections - http://omrf.ouhsc.edu/~frank/BACULPRO.html

Category: Recombinant Protein Protocols: Baculovirus Recombinant Protein

Generation of Recombinant Protein with Baculovirus Infections Protocols and Procedures. Frank Lab. - [Read Insect Cell Culture and Baculovirus Infections]

Insect Cell Culture and Baculovirus Infections Protocol - http://omrf.ouhsc.edu/~frank/BACULPRO.html

Category: Cell Culture Protocols: Insect Cell Culture Protocols

Protocol for insect cell culture and baculovirus infections. Includes: Cells, Media, Growth Characteristics, Freezing and Thawing Cells, and Glassware. - [Read Insect Cell Culture and Baculovirus Infections Protocol]

Inverse PCR for PAC-End Sequencing Protocol - http://www.genetics.wustl.edu/fish_lab/frank/cgi-bin/fish/prot2.html

Category: PCR Protocols: Inverse PCR Protocols

Protocol for inverse PCR for PAC-end sequencing. - [Read Inverse PCR for PAC-End Sequencing Protocol]

Isolation of T Cells from Synovial Membranes Protocol - http://omrf.ouhsc.edu/~frank/TCELLISO.html

Category: Immunology: Mononuclear Cell Isolation Protocols: T-Cell Isolation Protocols

Protocol for isolation of T cells from synovial membranes. Includes: Initial Cell Isolation; Nonadherent Cell Isolation; Adherent Cell Isolation; Neuraminidase-Treated Sheep Red Blood Cells; Erythrocyte Lysing Solution; Balance Salt Solution (BSS). - [Read Isolation of T Cells from Synovial Membranes Protocol]

PERSPECTIVES ON BACULOVIRUS EXPRESSION SYSTEMS - http://omrf.ouhsc.edu/~frank/baculsum.html

Category: Recombinant Protein Protocols: Baculovirus Recombinant Protein

PERSPECTIVES ON BACULOVIRUS EXPRESSION SYSTEMS. Advantages and disadvantages of protein expression in baculovirus . Insect Cell Culture. Frank Lab. - [Read PERSPECTIVES ON BACULOVIRUS EXPRESSION SYSTEMS]

Screening a Lambda Bacteriophage Genomic Library with a DNA Probe - http://omrf.ouhsc.edu/~frank/LIBSCRN.html

Category: RNA Protocols: cDNA Libraries Library

Frank Lab. Oklahoma Medical Research Foundation. How to screen a Lambda Bacteriophage Genomic Library with a DNA Probe. - [Read Screening a Lambda Bacteriophage Genomic Library with a DNA Probe]

Two Step real time PCR protocol - http://pga.tigr.org/sop/RT-PCR.pdf

Category: Real Time PCR

Two Step real time PCR protocol. Frank. - [Read Two Step real time PCR protocol]

Validation of Microarray Results Using Quantitative Real-Time PCR (QRT PCR) with Omniscript RT - http://omrf.ouhsc.edu/~frank/M_QPCR-OMNISCRIPT-RT.html

Category: Real Time PCR

Validation of Microarray Results Using Quantitative Real-Time PCR (QRT PCR) with Omniscript RT. Dr. Craig Cadwell - [Read Validation of Microarray Results Using Quantitative Real-Time PCR (QRT PCR) with Omniscript RT]