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Affymetrix Real Time PCR information and optimization - http://keck.med.yale.edu/affymetrix/rtpcr/

Affymetrix Real Time PCR information and optimization. Yale U. Keck Foundation. - [Read Affymetrix Real Time PCR information and optimization]

CALCIUM PHOSPATE TRANSFECTION OF HUMAN CELLS - http://omrf.ouhsc.edu/~frank/CAPO4TRANSFECT.html

Category: Cell Biology and Cell Culture: Cell Transfection Protocol

Simple Transfection using Calcium chloride and phosphate.Dr. Frank. Arthritis and Immunology Program, Oklahoma City. Medical Research Foundation. - [Read CALCIUM PHOSPATE TRANSFECTION OF HUMAN CELLS]

Detection of Recombinant Proteins by ELISA assay - http://omrf.ouhsc.edu/~frank/ELISA.html

Category: ELISA Protocols

Detection of Recombinant Proteins by ELISA protocol. Dr. Frank, Oklahoma Medical Research Foundation. - [Read Detection of Recombinant Proteins by ELISA assay]

Electroporation of Eukaryotic Cells - http://omrf.ouhsc.edu/~frank/EPOREUK.html

Category: Cell Biology and Cell Culture: Cell Electroporation Protocols

The protocol uses a Bio-Rad Gene Pulser II apparatus to electroporate DNA into human cell lines. Dr. Frank, Arthritis and Immunology Program, Oklahoma City Medical Research Foundation - [Read Electroporation of Eukaryotic Cells]

Immunoscreening of Lambda-GT11 Bacteriophage Libraries with Antibody Probes - http://omrf.ouhsc.edu/~frank/GT11.html

Category: RNA Protocols: cDNA Libraries Library

Adsroption of Anti-E. coli Antibodies, cDNA Library Screening, Frank Lab. Oklahoma Medical Research Foundation - [Read Immunoscreening of Lambda-GT11 Bacteriophage Libraries with Antibody Probes]

Multiparameter FACS Analysis - https://catalog.invitrogen.com/index.cfm?fuseaction=iProtocol.unitSectionTree&treeNodeID=9E661C9AB332DE6E4548834730AEA427&objectid=6674F9C194065C413146C81056933A0C

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Flow Cytometry Immunofluorescence Staining Protocols

Provides a brief historical perspective to illustrate the demands for this technology and to lay the foundation for its application; Explains the hurdles that were surmounted to achieve the current level of multiparametric analysis which serves to alert researchers to potential problems they may encounter when they either bring this technology to their own laboratories, or when they use extant instrumentation in another laboratory; Illustrates some of the complexities that arise. - [Read Multiparameter FACS Analysis]

RT In Situ PCR - Foundation of Success - http://www.bioscience.org/1996/v1/c/nuovo1/htmls/list.htm

Category: PCR Protocols: In-situ PCR Protocols

The key preparatory steps. In-situ RT-PCR. Fixative. Protease Digestion. DNase digestion. One-step in-situ RT PCR Protocol. Gerard J. Nuovo - [Read RT In Situ PCR - Foundation of Success]

Screening a Lambda Bacteriophage Genomic Library with a DNA Probe - http://omrf.ouhsc.edu/~frank/LIBSCRN.html

Category: RNA Protocols: cDNA Libraries Library

Frank Lab. Oklahoma Medical Research Foundation. How to screen a Lambda Bacteriophage Genomic Library with a DNA Probe. - [Read Screening a Lambda Bacteriophage Genomic Library with a DNA Probe]

The Basic Polymerase Chain Reaction Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3824

Category: PCR Protocols: Standard PCR Protocol

Protocol describes how to amplify a segment of double-stranded DNA in a chain reaction catalyzed by a thermostable DNA polymerase. It is the foundation for all subsequent variations of the polymerase chain reaction. - [Read The Basic Polymerase Chain Reaction Protocol]