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Extraction and analysis of diagnostically useful proteins from formalin-fixed, paraffin-embedded tis - http://www.jhc.org/cgi/content/full/46/3/397

Category: Protein Protocols: Protein Extraction From Tissue

Extraction and analysis of diagnostically useful proteins from formalin-fixed, paraffin-embedded tissue sections. Ikedaa et al, 1998 JHC. - [Read Extraction and analysis of diagnostically useful proteins from formalin-fixed, paraffin-embedded tis]

Extraction Method for Formalin Pigments from Tissue Sections Protocol - http://www.bio.com/protocolstools/protocol.jhtml?id=p1015

Category: Lipid Protocols

Protocol for extraction method for formalin pigments from tissue sections. - [Read Extraction Method for Formalin Pigments from Tissue Sections Protocol]

Immunohistochemistry Protocol - http://www.sigmaaldrich.com/Area_of_Interest/Life_Science/Antibody_Explorer/Procedures/Immunohistochemistry.html

Category: Immunohistochemistry Protocols

Protocol describes the application of peroxidase or alkaline phosphatase conjugates in the immunohistochemical labeling of formalin-fixed, paraffin-embedded tissue sections. Includes: Removal of Paraffin and Rehydration; Antigen Retrieval - Unmasking of Antigen; Enzyme retrieval; Microwave retrieval; Inactivation of Endogenous Peroxidase; etc.. - [Read Immunohistochemistry Protocol]

Immunohistochemistry Protocol for γH2AX Detection - http://www.natureprotocols.com/2006/12/06/immunohistochemistry_protocol.php

Category: Immunohistochemistry Protocols

Immunohistochemistry protocol for γH2AX detection (formalin-fixed paraffin-embedded sections). - [Read Immunohistochemistry Protocol for γH2AX Detection]

Preparing Frozen Tissue Sections for Immunostaining Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4328

Category: Immunohistochemistry Protocols: Antibody Staining Protocols

Frozen tissue sections show good preservation of tissue structure and antigens. The principle disadvantages of using them in immunostaining are that the specimens must be stored frozen, and a special microtome, known as a cryostat, is required. Also, many clinical specimens are not available in this form, and most classic histological descriptions of tissue structure and pathology are based on the use of paraffin-embedded sections of formalin-fixed material. - [Read Preparing Frozen Tissue Sections for Immunostaining Protocol]

Processing of Microdissected Tissue for Molecular Analysis - http://cgap-mf.nih.gov/Protocols/Processing/DNA.html

Category: Primary Cell Isolation and Culture Protocols: Laser Capture Microdissection Protocols

Information on processing of microdissected tissue for molecular analysis. Includes: More than 10,000 Cells; Less than 10,000 Cells; Formalin-fixed; Paraffin-embedded Tissue; Other Fixatives; Timing. - [Read Processing of Microdissected Tissue for Molecular Analysis]

Staining Manual and Fixing Manual. - http://medlib.med.utah.edu/WebPath/HISTHTML/MANUALS/MANUALS.html

Category: Histology Protocols: Histological Fixation

95% Alcoholic Bouin's Fixative, B5 Fixative, Formalin Fixative, Zenker's Fixative. Susan L. Wall Utah. - [Read Staining Manual and Fixing Manual.]

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Paraffin Embedding Protocol

Paraffin Embedding Protocol for molecular profiling. This Paraffin Embedding Protocol describes the processing of the tissues into sections following ethanol fixation. Molecular profiling (MP) is a technique that is used to visualize the global patterns of RNA expression or protein expression in various cell types and disease processes.