formaldehyde Protocols

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Apoptosis Detection Protocol By Single Laser Flow Cytometry - http://cyto.mednet.ucla.edu/Protocols/detection.htm

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols

A sensitive method for the detection of apoptosis by single laser flow cytometry. Methodology includes: Staining for detection of apoptosis, Direct Staining Procedure, Indirect Staining Procedure, Protocol for the use of actinomycin D (AD) on samples that were stained with 7-AAD for apoptosis and fixed in formaldehyde. - [Read Apoptosis Detection Protocol By Single Laser Flow Cytometry]

C. elegans Gonad Fixation Protocol - http://www.genetics.wustl.edu/tslab/Protocols/gonad_fixation.html

Category: C. elegans Protocols: C. elegans Fixing Protocols

Protocol for C.elegans gonad fixation. Includes fixation with: Methanol, Formaldehyde, Methanol / Formadehyde, Glutaraldehyde / Formaldehyde. - [Read C. elegans Gonad Fixation Protocol]

ChIP Assay Protocol PDF - http://www.bcm.edu/rosenlab/protocols/ChIP.pdf

Category: DNA Protocols: Chromatin Immunoprecipitation Protocols

Formaldehyde cross-linking and chromatin immunoprecipitation assays of tissue culture cells, Based on Boyd and Farnham. Michelle Kallesen, Rosen Lab. - [Read ChIP Assay Protocol PDF]

Chromatin Immunoprecipitation (ChIP) of Protein Complexes Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/23/pdb.prot4560

Category: Immunology: Immunoprecipitation Protocols: Chromatin Immunoprecipitation Protocols

Protocol describes the use of chromatin immunoprecipitation technology (ChIP) to analyze interactions of proteins or protein complexes with DNA in vivo. In this approach, the material is fixed with formaldehyde to preserve DNA-protein and protein-protein associations, the cells are lysed, and the chromatin is cut and solubilized. The chromatin suspension is immunoprecipitated with an antibody against the protein(s) of interest, and the coimmunoprecipitated DNA fragments are analyzed. - [Read Chromatin Immunoprecipitation (ChIP) of Protein Complexes Protocol]

CHROMATIN IMMUNOPRECIPITATION (CHIP) PROTOCOL FOR YEAST - http://www.ciwemb.edu/labs/koshland/Protocols/YEAST/chipmod.html

Category: DNA Protocols: Chromatin Immunoprecipitation Protocols

SPHEROPLAST CELLS, WASH SPHEROPLASTS, SONICATE SPHEROPLASTS, CHROMATIN SOLUTION, SET UP IMMUNOPRECIPITATIONS, REVERSE FORMALDEHYDE CROSSLINKS. Paul - Koshland lab. - [Read CHROMATIN IMMUNOPRECIPITATION (CHIP) PROTOCOL FOR YEAST]

Chromatin IP (CHIP assay) - http://www.fhcrc.org/science/labs/breeden/Methods/chromatinIP.html

Category: DNA Protocols: Chromatin Immunoprecipitation Protocols

ChIP assay protocol with 2 steps: in vivo formaldehyde cross-linking of whole cells and protein-protein and protein-DNA interactions, followed by immunoprecipitation of protein-DNA complexes with specific antibodies from sonicated extracts. Breeden Lab - [Read Chromatin IP (CHIP assay)]

Dot and Slot Hybridization of Purified RNA Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot4052

Category: Nucleic Acid Detection Protocols: Nucleic Acid Blotting Protocols

Protocol for dot and slot hybridization of purified RNA. Dot blotting of RNA is best carried out using purified preparations of RNA that are denatured with glyoxal or formaldehyde immediately before loading onto a nylon membrane through a vacuum manifold. - [Read Dot and Slot Hybridization of Purified RNA Protocol]

Fixation. - http://www.dakousa.com/ishbfixation.pdf

Category: Histology Protocols: Histological Fixation

FORMALDEHYDE-BASED FIXATIVES, PARAFFIN-EMBEDDED SECTIONS, BOUINâ€™S SOLUTION, MERCURIC CHLORIDE-BASED FIXATIVES. FARMILO et al. - [Read Fixation.]

Fixing Attached Cells in Paraformaldehyde Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/21/pdb.prot4294

Category: Cell Culture Protocols: Cell Fixing Protocols

Treating cells with paraformaldehyde leads to the establishment of chemical cross-links between free amino groups. When the cross-links join different molecules, a latticework of interactions occurs that holds the overall architecture of the cell together. Commercial formaldehyde solutions are not recommended, because they lack the advantages of using a variable-length polymer, and the cells will simultaneously be fixed with the alcohol (usually methanol). - [Read Fixing Attached Cells in Paraformaldehyde Protocol]

Formaldehyde Treatment of Tissue Culture Hoods - http://www.nottingham.ac.uk/~mbzspd/methods/Formaldehyde_Treatme.html

Category: General Research Protocols and Methods: Sterile Technique

Formaldehyde sterilization of tissue culture hoods. A bit toxic however effective. Dr.Dawson, Department Biochemistry, Univ. Nottingham Medical School. - [Read Formaldehyde Treatment of Tissue Culture Hoods]

Immunofluorescence protocol - Formaldehyde fixation - http://www.immuquest.com/cexe/protocols.asp?ID=19

Category: Molecular Imaging: Immunofluorescence Microscopy

Immunofluorescence protocol - Formaldehyde fixation. Immunoquest. - [Read Immunofluorescence protocol - Formaldehyde fixation]

Measurement of GFP Expression and DNA Content in Permeabilized Cells - http://cyto.mednet.ucla.edu/Protocols/DNA%20and%20GFP%20PI.htm

Category: Reporter Gene Assays: GFP Assay Protocols

Protocol measuring the amount of GFP expression and DNA content in permeabilized cells. Includes: Fix cells with formaldehyde; Permeabilize cells with ethanol; Preparation of Buffered 2% Formaldehyde Solution. - [Read Measurement of GFP Expression and DNA Content in Permeabilized Cells]

Measurement of GFP Expression and DNA Content in Permeabilized Cells Protocol - http://cyto.mednet.ucla.edu/Protocols/DNA%20and%20GFP%20PI.htm

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Flow Cytometry Data Analysis Protocols

Protocol for the measurement of GFP expression and DNA content in permeabilized cells. Includes: Fix cells with formaldehyde; Permeabilize cells with ethanol; Preparation of Buffered 2% Formaldehyde Solution. - [Read Measurement of GFP Expression and DNA Content in Permeabilized Cells Protocol]

Preparation of a Denaturing Agarose Gel for the Analysis of RNA Samples Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/14/pdb.prot4127

Category: Nucleic Acid Electrophoresis Protocols: RNA Electrophoresis Protocols

Protocol for preparation of a denaturing agarose gel for the analysis of RNA samples. The integrity of RNA samples can be analyzed by electrophoresis on a denaturing agarose gel containing formaldehyde. - [Read Preparation of a Denaturing Agarose Gel for the Analysis of RNA Samples Protocol]

Preparing Early Whole-Mount Drosophila Embryos for Immunostaining Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/23/pdb.prot4524

Category: Drosophila Protocols: Drosophila Staining Protocols

Early embryos (0-17 hours or until cuticle formation) are treated with a mixture of organic solvents, formaldehyde, and alcohols, as described here. The cuticles of late-stage embryos are usually opened by sonication. Tissues from more advanced stages of development are normally dissected by hand and then fixed and stained in a standard paraformaldehyde/detergent combination - [Read Preparing Early Whole-Mount Drosophila Embryos for Immunostaining Protocol]

Preparing Late Whole-Mount Drosophila Embryos for Immunostaining Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/23/pdb.prot4525

Category: Drosophila Protocols: Drosophila Staining Protocols

Early and late embryos are treated with a mixture of organic solvents, formaldehyde, and alcohols. The cuticles of late-stage embryos (17-22 hours or until hatching) are usually opened by sonication, as described here. Tissues from later stages of development are normally dissected by hand and then fixed and stained in a standard paraformaldehyde/detergent combination. - [Read Preparing Late Whole-Mount Drosophila Embryos for Immunostaining Protocol]

Separation of RNA According to Size: Electrophoresis of RNA through Agarose Gels Containing Formalde - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot4050

Category: Nucleic Acid Electrophoresis Protocols: RNA Electrophoresis Protocols

Separation of RNAs according to size is the first stage in northern blotting and hybridization. The method described in this protocol uses formaldehyde to denature the RNA, ethidium bromide to stain it, and electrophoresis through agarose gels containing 2.2 M formamide to separate the resulting formaldehyde-RNA-ethidium adducts. - [Read Separation of RNA According to Size: Electrophoresis of RNA through Agarose Gels Containing Formalde]