fluorophore Protocols

Search results for: fluorophore

Protocols » Search Results

Search Results Protocol Links Sort by: Hits | Alphabetical

Band Shift Protocols - http://www.pharma.ethz.ch/institute_groups/biomacromolecules/protocols/bandshift

Category: DNA Protein Interactions Protocols: Electrophoretic Mobility Shift Assays EMSA Protocols

Protocols on band shift. Includes: Phosphorylation of recombinant antibodies; Labeling the antigen with a red fluorophore; Native gel electrophoresis; Band-shift assay for the determination of Kd; Band-shift assay for the determination of koff. - [Read Band Shift Protocols]

Immunofluorescence Staining Protocol - https://catalog.invitrogen.com/index.cfm?fuseaction=iProtocol.unitSectionTree&treeNodeID=9E662E4DA09244177634DA520E076CB6&objectid=6673C6FEF0EF1F391890AEA1A3AE9FBD

Category: Immunology: Immunofluorescence Protocols: Immunofluorescence Staining Protocols

Provides a protocol for indirect immunofluorescence, which is a method that provides information about the locations of specific molecules and the structure of the cell. Antibody molecules for a specific target molecule are exposed to the cell or tissue being investigated. The binding of these molecules is detected by incubating the sample with a secondary antibody specific for immunoglobulin molecules and conjugated to fluorophore. - [Read Immunofluorescence Staining Protocol]

Introduction to Live-Cell Imaging Techniques - http://microscopy.fsu.edu/primer/techniques/livecellimaging/index.html

Category: Microscopy Protocols: Live-Cell Imaging Protocols

Live-cell imaging techniques provide critical insight into the fundamental nature of cellular & tissue function, especially due to the rapid advances that are currently being witnessed in fluorescent protein & synthetic fluorophore technology. Because of these advances, live-cell imaging has become a requisite analytical tool in most cell biology labs. Includes: Maintaining Live Cells on the Microscope Stage; Live-Cell Imaging Culture Chambers; Optical System and Detector Requirements etc. - [Read Introduction to Live-Cell Imaging Techniques]

Maintaining Live Cells on the Microscope Stage - http://www.microscopyu.com/articles/livecellimaging/livecellmaintenance.html

Category: Microscopy Protocols: Live-Cell Imaging Protocols

Live-cell imaging techniques provide a critical insight into the fundamental nature of cellular and tissue function, especially due to the rapid advances that are currently being witnessed in fluorescent protein and synthetic fluorophore technology. Because of these advances, live-cell imaging has become a requisite analytical tool in most cell biology laboratories. - [Read Maintaining Live Cells on the Microscope Stage]

Order Fluorophore Wall Chart - http://microscopy.bio-rad.com/fluorescence/fluorochrome.htm

Category: Molecular Imaging

Order Fluorophore Wall Chart. Free. CellScience - [Read Order Fluorophore Wall Chart]

Probing Protein Interactions Using GFP and FRET - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3822

Category: Reporter Gene Assays: GFP Assay Protocols

Protocol specifically describes data acquisition for a particular variant of GFP (EGFP) or Oregon Green as a donor fluorophore, but it can be adapted for image acquisition of other chromophore. - [Read Probing Protein Interactions Using GFP and FRET]