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Protocol for Standard Fluorescence Motility Assay - http://www.proweb.org/kinesin/Methods/motility.html

Protocol for Standard Fluorescence Motility Assay. Materials requires: Microtubules, Flow Cell, Standard Solutions: BRB80, BRB80T, BRB80CS0.5, BRB80CA, BRB80CT - [Read Protocol for Standard Fluorescence Motility Assay]

Purification of Lin-, c-kit+, Sca-1+ Bone Marrow Cells for Culture, Flow Cytometry, or Transplantaio - http://www.fhcrc.org/science/labs/fero/Protocols/Hematopoietic.pdf

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols

Protocol for purification of Lin-, c-kit+, Sca-1+ bone marrow cells for Culture, Flow Cytometry, or Transplantaion. Includes: Antibodies for Lineage Depletion; Harvest Marrow; Lineage Depletion; Cell Sorting; Viral Transduction; Transplant; CFU assays; GFP and cell surface marker immunostaining. - [Read Purification of Lin-, c-kit+, Sca-1+ Bone Marrow Cells for Culture, Flow Cytometry, or Transplantaio]

Purification of Lin-, c-kit+, Sca-1+ Bone Marrow Cells Protocol - http://www.fhcrc.org/science/labs/fero/Protocols/Hematopoietic.pdf

Category: Immunology

Protocol for purification of Lin-, c-kit+, Sca-1+ bone marrow cells for Culture, Flow Cytometry, or Transplantaion. Includes: Harvest Marrow; Lineage Depletion; Cell Sorting; Viral Transduction; Transplant; CFU assays; GFP and cell surface marker immunostaining. - [Read Purification of Lin-, c-kit+, Sca-1+ Bone Marrow Cells Protocol]

Quantification of Apoptosis and Cell Cycle Distribution of Primary B Cells Using Propidium Iodide - http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi/afcs_PP00000024.pdf?pid=PP00000024

Category: Immunology: B Cell Protocols

The water soluble, DNA intercalator, propidium iodide (PI), is used to bind to DNA after permeabilization of cells with NP40. The amount of dye bound correlates with the content of DNA within a given cell. Once cells are stained, they are analyzed on a flow cytometer. The relative content of DNA indicates the distribution of a population of cells throughout the cell cycle. - [Read Quantification of Apoptosis and Cell Cycle Distribution of Primary B Cells Using Propidium Iodide]

Quantification of Apoptosis and the Cell Cycle Distribution of Primary B Cells Using PI - http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi/afcs_PP00000024.pdf?pid=PP00000024

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Apoptosis Cytometry Protocols

The water soluble, DNA intercalator, propidium iodide (PI), is used to bind to DNA after permeabilization of cells with NP40. The amount of dye bound correlates with the content of DNA within a given cell. Once cells are stained, they are analyzed on a flow cytometer. The relative content of DNA indicates the distribution of a population of cells throughout the cell cycle. - [Read Quantification of Apoptosis and the Cell Cycle Distribution of Primary B Cells Using PI]

Quantitative Flow Cytometry for Apoptosis - http://www.biotech.uiuc.edu/centers/Proteomics/Flow_cytometry/docs/Apopbroc.pdf

Category: Cell Biology and Cell Culture: Apoptosis Protocols

Quantitative Flow Cytometry for Apoptosis Detection. Flow Cytometry Facility, U.Illinois. - [Read Quantitative Flow Cytometry for Apoptosis]

Rat Hepatocyte Flow Cytometric Cytotoxicitiy Test - http://embryo.ib.amwaw.edu.pl/invittox/prot/23.htm#TOP

Category: Pharmacology and Toxicology Protocols: Cytotoxicity Protocols: Liver Toxicity Assays Protocols

Flow cytometry is used to monitor drug-induced changes in DNA and protein contents of hepatocytes cultured at physiological oxygen concentrations. - [Read Rat Hepatocyte Flow Cytometric Cytotoxicitiy Test]

Separation of Platelets from Whole Blood - http://www.cbrinstitute.org/labs/springer/protocols/azucena_platelets.html

Category: Cell Biology and Cell Culture

This protocol describes how to isolate human platelets from whole blood. Isolated platelets are used for static adhesion assays, for flow chamber assays, flow cytometry measurements, etc. - [Read Separation of Platelets from Whole Blood]

Sodium and Potassium Analysis by Flow Cytometry Protocol - http://www.niehs.nih.gov/research/atniehs/core/fc/protocols/sodium.cfm

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols

Protocol for sodium and potassium analysis by flow cytometry. Includes: Preparation of the dyes; Loading of the dyes; Flow Cytometric Analysis. - [Read Sodium and Potassium Analysis by Flow Cytometry Protocol]

Sodium and Potassium Analysis by Flow Cytometry Protocol - http://www.niehs.nih.gov/research/atniehs/core/fc/protocols/sodium.cfm

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Intracellular Ion Measurement Protocols

The Use of Flow Cytometry for Concomitant Detection of Apoptosis and Cell Cycle Analysis - http://www.roche-applied-science.com/PROD_INF/BIOCHEMI/No.2_96/pp25-27.pdf

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Apoptosis Cytometry Protocols

Two distinct modes of cell death, apoptosis and necrosis, can be distinguished on the basis of differences in morphological, biochemical, and molecular changes occurring in the dying cells. Report the development of flow cytometric techniques that permit concomitant detection of apoptosis and cellular DNA content or BrdU content analysis. - [Read The Use of Flow Cytometry for Concomitant Detection of Apoptosis and Cell Cycle Analysis]

Transfer and Fixation of Denatured RNA to Membranes Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot4060

Category: Nucleic Acid Electrophoresis Protocols: RNA Electrophoresis Protocols

Protocol describes the transfer of RNA from agarose gels to neutral or positively charged nylon membranes, using upward capillary flow of neutral or alkaline buffers. RNA becomes covalently fixed to positively charged nylon membranes during transfer in alkaline buffers. However, treatment by UV irradiation or heating is required to fix RNA to neutral membranes. - [Read Transfer and Fixation of Denatured RNA to Membranes Protocol]

Whatman Protein Precipitation UNIFILTER Fast Flow - http://www.whatman.com/repository/documents/s7/WGP_169_A.pdf

Category: Protein Protocols: Protein Precipitation Procols

Whatman Protein Precipitation UNIFILTER Fast Flow. - [Read Whatman Protein Precipitation UNIFILTER Fast Flow]

Yeast Cell Cycle by Flow Cytometry Protocol - http://pingu.salk.edu/flow/protocols/ycc.html

Category: Cell Biology and Cell Culture: Cell Cycle Protocols

Protocol for yeast cell cycle by flow cytometry. Includes procedure for dyes: Propidium Iodide and Sytox Green. - [Read Yeast Cell Cycle by Flow Cytometry Protocol]

Yeast Cell Cycle by Flow Cytometry Protocol - http://pingu.salk.edu/flow/protocols/ycc.html

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Cell Cycle Analysis Protocols

Protocol for yeast cell cycle by flow cytometry. - [Read Yeast Cell Cycle by Flow Cytometry Protocol]

Yeast Cell Cycle by Flow Cytometry Protocol - http://pingu.salk.edu/flow/protocols/ycc.html

Category: Yeast Protocols

Protocol for Yeast cell cycle by flow cytometry. - [Read Yeast Cell Cycle by Flow Cytometry Protocol]

Articles (1-3 of 3)

Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA

This Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA protocol describes the production of probes labeled with the fluorescent dyes, Cy3 and Cy5, following the synthesis of cDNA from human mRNA and the hybridization of the probes to DNA microarrays.

Coupling Cysteine Containing Peptides to Carrier Protein for Immunization and Polyclonal Antibody

This protocol is used in the production of polyclonal antibodies that recognize a peptide sequence of interest.

Picking Transfected ES Cell Clones Protocol

This protocol a protocol on how to generate transfected embryonic stem (ES) cell clones. The previous protocol in this series is the Protocol for Electroporation of ES cells. The next protocol in the series is the Protocol on Disaggregation, Expansion, and Freezing of Transfected ES Clones.

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