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Negative Stain Electron Microscopy of Microtubules Protocol - http://mitchison.med.harvard.edu/protocols/EM.html

Category: Cytoskeleton Protocols: Microtubules Protocols

Negative staining is a rapid, qualitative method for analyzing microtubule structure at the EM level. Because negative staining involves deposition of heavy atom stains, structural artifacts such as flattening of the cylindrical microtubule and opening up of microtubules into flat sheets are common. Negative staining is very useful because of its ease, rapidity and lack of requirement for specialized equipment other than that found in a regular EM facility. - [Read Negative Stain Electron Microscopy of Microtubules Protocol]

Staining Resin Sections of Bone Protocol - http://www.nottingham.ac.uk/pathology/protocols/resbonestain.html

Category: Immunohistochemistry Protocols: Resin Staining Protocols

Protocol for staining resin sections of bone. Includes: VON KOSSA/VAN GIESON; GOLDNER TRICHROME (MODIFICATION); TOLUIDINE BLUE; PERLS PRUSSIAN BLUE; SECTIONS LABELLED WITH TETRACYCLINE; FLATTENING FREE-FLOATING SECTIONS; MOUNTING FREE-FLOATING SECTIONS; CLEANING SLIDES. - [Read Staining Resin Sections of Bone Protocol]

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Picking Transfected ES Cell Clones Protocol

This protocol a protocol on how to generate transfected embryonic stem (ES) cell clones. The previous protocol in this series is the Protocol for Electroporation of ES cells. The next protocol in the series is the Protocol on Disaggregation, Expansion, and Freezing of Transfected ES Clones.