User Panel

My Panel

Bookmark Science Articles

fixation Protocols

Search results for: fixation

Protocols » Search Results

Search Results Protocol Links Sort by: Hits | Alphabetical

Fixation and Separation of Arabidopsis Leaf Cells Protocol - http://www.bio.com/protocolstools/protocol.jhtml%3Bjsessionid%3DM03FJWU3MGILNR3FQLMSFEWHUWBNQIV0?id=p1095

Category: Plant Biology Protocols

Protocol for the fixation and seperation of Arabidopsis leaf cells. Includes: Sample Preparation; Slide Preparation. - [Read Fixation and Separation of Arabidopsis Leaf Cells Protocol]

Fixation and Staining Parameters - http://www-biology.ucsd.edu/labs/aroian/protocols/fixatives.htm

Category: C. elegans Protocols: C. elegans Fixing Protocols

Parameters for fixation and staining. Includes: Antigen, Fixation, Fixation time, Antibody and Dilution. - [Read Fixation and Staining Parameters]

Fixation of Mouse Embryos and Tissues Protocol - http://www.cshprotocols.org/cgi/content/abstract/2007/1/pdb.prot4702

Category: Mouse Protocols: Mouse Embryology Protocols

Protocol provides methods for fixation of mouse embryos and tissues with paraformaldehyde, Bouin’s fixative, or methanol/DMSO solution. - [Read Fixation of Mouse Embryos and Tissues Protocol]

Fixation of Plant Metaphase Chromosomes Protocol - http://www.le.ac.uk/biology/phh4/fixation.htm

Category: Plant Biology Protocols: Plant Genetics Protocols

Protocol for the fixation of plant metaphase chromosomes. - [Read Fixation of Plant Metaphase Chromosomes Protocol]

Fixation of Xenopus Embryos - http://tropicalis.berkeley.edu/home/manipulate_embryos/fixation.html

Category: Xenopus Protocols: Xenopus Embryo Manipulation Protocols

Protocol for the fixation of xenopus embryos. - [Read Fixation of Xenopus Embryos]

Fixation Protocol - http://cmif.osu.edu/419.cfm

Category: Histology Protocols: Fixation of Cells Tissues Protocol

There are many variations based on the type of tissue to be examined, whether one is dealing with cell suspensions, biopsies, perfused tissues, or monolayer . Fixation Protocol. OSU Campus Microscopy & Imaging Facility :: The Ohio State University College of Med - [Read Fixation Protocol]

FIXATION PROTOCOL FOR FLOW CYTOMETRIC ANALYSIS OF DNA BY PI STAINING - http://www.vetmed.auburn.edu/index.pl/cell_cycle/dna_content

Category: Histology Protocols: Fixation of Cells Tissues Protocol

Analysis of Cell Cycle Based on DNA Content. FIXATION PROTOCOL FOR FLOW CYTOMETRIC ... Liberate cells from tissue culture flask by trypsin digestion and ... FIXATION PROTOCOL FOR FLOW CYTOMETRIC ANALYSIS OF DNA BY PI STAINING. Auburn University, College of Veterinary Medicine. You. - [Read FIXATION PROTOCOL FOR FLOW CYTOMETRIC ANALYSIS OF DNA BY PI STAINING]

Fixation, permeabilization and immunostaining of cells - http://www.lamondlab.com/pdf/Fix&Stain.pdf

Category: Immunology: Immunocytochemistry Protocols

Fixation, permeabilization and immunostaining of cells. Lamond Lab, Univ. Dundee - [Read Fixation, permeabilization and immunostaining of cells]

Fixation. - http://www.dakousa.com/ishbfixation.pdf

Category: Histology Protocols: Histological Fixation

FORMALDEHYDE-BASED FIXATIVES, PARAFFIN-EMBEDDED SECTIONS, BOUINâ€™S SOLUTION, MERCURIC CHLORIDE-BASED FIXATIVES. FARMILO et al. - [Read Fixation.]

Fixing Caenorhabditis elegans in Bouin’s Fixative Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/23/pdb.prot4521

Category: C. elegans Protocols: C. elegans Fixing Protocols

Bouin’s fixative is a particularly good choice for worms because it penetrates dense tissues well and is extremely good for fixing antigens. Like all strong fixatives, however, it is unsuitable for some antibody-antigen pairs. In such cases, the length of time in the Bouin’s fixative can be shortened, or paraformaldehyde fixation can be used instead. - [Read Fixing Caenorhabditis elegans in Bouin’s Fixative Protocol]

Fixing Caenorhabditis elegans in Paraformaldehyde Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/23/pdb.prot4522

Category: C. elegans Protocols: C. elegans Fixing Protocols

Common method for fixing worms is to use paraformaldehyde. This method provides a gentler fixation than the Bouin’s method, but often requires the use of collagenase. This method is particularly good for examining adult worms. - [Read Fixing Caenorhabditis elegans in Paraformaldehyde Protocol]

General Overview of Histology - http://www-bioc.rice.edu/bios576/histology/histology.htm

Category: Histology Protocols: Fixation of Cells Tissues Protocol

Fixation must convert the soluble contents of the cell (ie proteins, carbohydrates, ... http://www.protocol-online.org/prot/Histology/Fixation/ ...General Overview of Histology and Tissue Fixation. BIOS 576. Rice. - [Read General Overview of Histology]

GFP Fixation in Yeast Protocol - http://www.ciwemb.edu/labs/koshland/Protocols/MICROSCOPY/gfpfix.html

Category: Yeast Protocols: Yeast Staining Protocols

Protocol for GFP fixation in Yeast. - [Read GFP Fixation in Yeast Protocol]

Histological Fixatives - http://www.bristol.ac.uk/vetpath/cpl/histfix.htm

Category: Histology Protocols: Histological Fixation

Fixation, Classification, REMOVAL OF FIXATION PIGMENTS, Fixative recipes, Mercury pigment, Dichromate pigment. Univ. Bristol Veterinary. - [Read Histological Fixatives]

HISTOLOGY-RELATED PROTOCOLS Fixation and Frozen Block PDF - http://www.uchsc.edu/misc/diabetes/derc/protocols/histologyprotocols.pdf

Category: Histology Protocols: Fixation of Cells Tissues Protocol

Protocols for cell fixation and tissue fixation. HISTOLOGY-RELATED PROTOCOLS Fixation and Frozen Block techniques. Embedding Tissue Into Frozen Blocks. Fixing Cells with Fresh Paraformaldehyde. Preparation of Frozen Sections. Immunohistochemistry. Paraffin sections and Frozen sections. DERC Univ. Colorado PDF. - [Read HISTOLOGY-RELATED PROTOCOLS Fixation and Frozen Block PDF]

Histotechniques - Manual - http://medlib.med.utah.edu/WebPath/HISTHTML/HISTOTCH/HISTOTCH.html

Category: Histology Protocols

Histotechniques - Manual. Great Histology Protocol Handbook. Includes: Tissue Processing, Specimen Accessioning, Fixation, Fixatives, Tissue Processing, Sectioning, H and E staining, more. - [Read Histotechniques - Manual]

Histotechniques Fixation - http://library.med.utah.edu/WebPath/HISTHTML/HISTOTCH/HISTOTCH.html

Category: Immunohistochemistry Protocols: Fixation Protocols

Information on histotechniques. Includes: Fixation - types of fixatives; factors affecting fixation; General usage of fixatives; Tissue Processing; Sectioning; Frozen Sections; Staining; H and E staining; Coverslipping; Decalcification; Artefacts in Histologic Sections; Problems in Tissue Processing. - [Read Histotechniques Fixation]

Identification of Single Bacterial Cells using DIG-Labeled Oligonucleotides Protocol - http://www.roche-applied-science.com/PROD_INF/MANUALS/InSitu/pdf/ISH_139-142.pdf

Category: Cytogenetics Protocols: DIG Biotin Labeled Probe Protocols

Protocol for the identification of single bacterial cells using DIG-labeled oligonucleotides. Includes: Organisms and growth conditions; Cell fixation and preparation of cell smears; DIG labeling of oligonucleotides with DIG-ddUTP; In situ hybridization using digoxigenin-labeled oligonucleotides; Detection of DIG-labeled oligonucleotides with fluorescently labeled anti-DIG Fab fragments; Detection of DIG-labeled oligonucleotide. - [Read Identification of Single Bacterial Cells using DIG-Labeled Oligonucleotides Protocol]

Identification of Single Bacterial Cells Using DIG-Labeled Oligonucleotides Protocol - http://www.roche-applied-science.com/PROD_INF/MANUALS/InSitu/pdf/ISH_139-142.pdf

Category: Nucleic Acid Modification Protocols: DIG Biotin Labeled Probes Protocols

Protocol for identification of single bacterial cells using DIG-labeled oligonucleotides. Includes: Organisms and growth conditions; Cell fixation and preparation of cell smears; DIG labeling of oligonucleotides with DIG-ddUTP; In situ hybridization using digoxigenin-labeled oligonucleotides. - [Read Identification of Single Bacterial Cells Using DIG-Labeled Oligonucleotides Protocol]

Imaging of Fresh Arabidopsis Tissues in the Scanning Electron Microscope - http://www.cshprotocols.org/cgi/content/abstract/2007/1/pdb.prot4686

Category: Microscopy Protocols: Electron Microscopy Protocols

In recent years, the increased sensitivity of electron detectors and the availability of low-vacuum or variable-pressure systems have allowed imaging of fresh tissue samples without the need for fixation, drying, and coating. This obviously saves a lot of time, although the image quality may not be as good as that obtained from fixed samples. However, for most applications that tend to be at a relatively low magnification, the quality can be as good as that obtained from fixed samples. - [Read Imaging of Fresh Arabidopsis Tissues in the Scanning Electron Microscope]

Imaging of Fresh Arabidopsis Tissues in the Scanning Electron Microscope Protocol - http://www.cshprotocols.org/cgi/content/abstract/2007/1/pdb.prot4686

Category: Plant Biology Protocols

Immuno-Laser Capture Microdissection Protocol - http://cgap-mf.nih.gov/Protocols/Slides/SlideProtocols/ImmunoLCM.html

Category: Primary Cell Isolation and Culture Protocols: Laser Capture Microdissection Protocols

After fixation, frozen sections are immunostained under RNase-free conditions using a rapid three-step streptavidin-biotin technique followed by dehydration. The immunostained sections are ready for LCM. Includes: Development of Immuno-LCM. - [Read Immuno-Laser Capture Microdissection Protocol]

Immunofluorescence - http://www.kcl.ac.uk/depsta/biomedical/randall/methods/im-fluo.html

Category: Molecular Imaging: Immunofluorescence Microscopy

Immunofluorescence. Dr. Walter Steffen. Includes fixation and staining protocols. - [Read Immunofluorescence]

Immunofluorescence Labeling of Cells Protocol - http://www.sigmaaldrich.com/Area_of_Interest/Life_Science/Antibody_Explorer/Procedures/Immunofluorescence.html

Category: Immunology: Immunofluorescence Protocols

Protocol for immunofluorescence labeling of cells. Includes: Cell Preparation; Fixation; Application of Primary Antibody; Application of Secondary Antibody and Evaulation. - [Read Immunofluorescence Labeling of Cells Protocol]

Immunofluorescence protocol - Formaldehyde fixation - http://www.immuquest.com/cexe/protocols.asp?ID=19

Category: Molecular Imaging: Immunofluorescence Microscopy

Immunofluorescence protocol - Formaldehyde fixation. Immunoquest. - [Read Immunofluorescence protocol - Formaldehyde fixation]

Articles (1-9 of 9)

Paraffin Embedding Protocol

Paraffin Embedding Protocol for molecular profiling. This Paraffin Embedding Protocol describes the processing of the tissues into sections following ethanol fixation. Molecular profiling (MP) is a technique that is used to visualize the global patterns of RNA expression or protein expression in various cell types and disease processes.