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4% Paraformaldehyde Protocol - http://www.nottingham.ac.uk/pathology/protocols/paraform.html

Category: Immunohistochemistry Protocols: Fixation Protocols

Protocol for 4% paraformaldehyde. Includes: 4% PARAFORMALDEHYDE/PBS; 0.4% PARAFORMALDEHYDE/PBS (FOR POST-DIGESTION FIXATION). - [Read 4% Paraformaldehyde Protocol]

70% Ethanol Fixation for Recovery of DNA, RNA, and Protein Protocol - http://cgap-mf.nih.gov/Protocols/Tissues/TissueProtocols/EthanolFixation.html

Category: Primary Cell Isolation and Culture Protocols: Laser Capture Microdissection Protocols: Tissue Preparation for LCM Protocols

Protocol for 70% ethanol fixation for recovery of DNA, RNA, and protein. - [Read 70% Ethanol Fixation for Recovery of DNA, RNA, and Protein Protocol]

70% Ethanol Fixation for Recovery of DNA, RNA, and Protein Protocol - http://cgap-mf.nih.gov/Protocols/Tissues/TissueProtocols/EthanolFixation.html

Category: Primary Cell Isolation and Culture Protocols

Protocol for 70% ethanol fixation for recovery of DNA, RNA and protein. - [Read 70% Ethanol Fixation for Recovery of DNA, RNA, and Protein Protocol]

Accumulation and Fixation of Plant Metaphase Chromosomes Protocol - http://www.le.ac.uk/bl/phh4/proaccum.htm

Category: Cytogenetics Protocols

Protocol for accumulation and fixation of plant metaphase chromosomes. - [Read Accumulation and Fixation of Plant Metaphase Chromosomes Protocol]

Accumulation and Fixation of Plant Metaphase Chromosomes Protocol - http://www.le.ac.uk/bl/phh4/proaccum.htm

Category: Plant Biology Protocols: Plant Genetics Protocols

For analysis of metaphase chromosomes, any tissue containing dividing cells can be used: Root tips from young seedlings, from newly grown roots at the edge of plant pots or hydroponic culture are all suitable. Alternatively, flower buds, anthers, carpels or leaf or apical meristems can be used. Includes metaphase arresting reagents. - [Read Accumulation and Fixation of Plant Metaphase Chromosomes Protocol]

Alkaline Phosphatase (APAAP) Technique - http://www.nottingham.ac.uk/pathology/protocols/apap.html

Category: Signalling Signal Transduction Protocols

Technique for Alkaline Phosphatase (APAAP). Techniques includes information on: Cytological Preparations, Fixation, Technique, Results, Substrate Solution and 0.005M Tris/HC1 Saline Buffer pH 7.6. - [Read Alkaline Phosphatase (APAAP) Technique]

Alkaline Phosphatase (APAAP) Technique Protocol - http://www.nottingham.ac.uk/pathology/protocols/apap.html

Category: Immunohistochemistry Protocols: Antibody Staining Protocols

Protocol for alkaline phosphatase (APAAP) technique. Includes: Fixation; Technique; Substrate Solution. - [Read Alkaline Phosphatase (APAAP) Technique Protocol]

Analysis of DNA Content and Surface Immunophenotype Protocol Using Ethanol Fixation - http://www.cyto.purdue.edu/flowcyt/research/cytotech/telford/ethanol.htm

Category: Immunohistochemistry Protocols: Fixation Protocols

Protocol for analysis of DNA content and surface immunophenotype using gentle ethanol fixation techniques. - [Read Analysis of DNA Content and Surface Immunophenotype Protocol Using Ethanol Fixation]

Analysis of DNA Fragmentation Using Propidium Iodide (PI) Staining After Ethanol Fixation Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4431

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: DNA Staining Protocols for Flow Cytometry

This protocol provides a method for analysis of DNA fragmentation using flow cytometry after propidium iodide (PI) labeling of fixed apoptotic cells. Flow cytometry reveals apoptotic nuclei in the subdiploid region of the cell cycle histogram... - [Read Analysis of DNA Fragmentation Using Propidium Iodide (PI) Staining After Ethanol Fixation Protocol]

Attaching Suspension Cells to Slides Using a Cytocentrifuge Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/21/pdb.prot4291

Category: Cell Culture Protocols: Cell Staining Protocols

A simple method for handling suspension cells is to attach them to a solid substrate before fixation, and one way to do this is to use a cytocentrifuge. - [Read Attaching Suspension Cells to Slides Using a Cytocentrifuge Protocol]

Autoradiography and Reading of Sequencing Gels Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3588

Category: DNA Protocols: DNA Sequencing Protocols

Protocol describes fixation of a sequencing gel and processingin preparation for autoradiography. - [Read Autoradiography and Reading of Sequencing Gels Protocol]

C. elegans Gonad Fixation Protocol - http://www.genetics.wustl.edu/tslab/Protocols/gonad_fixation.html

Category: C. elegans Protocols: C. elegans Fixing Protocols

Protocol for C.elegans gonad fixation. Includes fixation with: Methanol, Formaldehyde, Methanol / Formadehyde, Glutaraldehyde / Formaldehyde. - [Read C. elegans Gonad Fixation Protocol]

Cell Fixation Using Methanol - http://www.lamondlab.com/pdf/CellFixMEOH.pdf

Category: Immunology: Immunocytochemistry Protocols

Methanol Fixation for Immunofluorescence. Lamond Lab, Univ. Dundee - [Read Cell Fixation Using Methanol]

Cell Staining for Immunofluorescence Microscopy - http://www.bdbiosciences.com/pharmingen/protocols/Immunofluorescence_Microscopy.shtml

Category: Molecular Imaging: Immunofluorescence Microscopy

Cell Staining for Immunofluorescence Microscopy. Includes protocols for fixing the cells, Coverslip Preparation for Adherent Cells, Coverslip Preparation for Non-Adherent Cells, Paraformaldehyde Fixation, and Methanol/Acetone Fixation. Blocking protocols include blocking with primary antibody, and incubation with secondary antibody. - [Read Cell Staining for Immunofluorescence Microscopy]

Confocal Microscopy and Protocols - http://depts.washington.edu/keck/intro.htm

Category: Molecular Imaging: Confocal Microscopy Protocols and Information

Confocal Microscopy and Protocols. Why use a confocal microscope? Fluorescence, Reflectance or Transmission? Which confocal microscope should you use? Confocal or 2-photon microscopy? Sample Preparation for confocal microscopy, Fixation, Immunolabeling, - [Read Confocal Microscopy and Protocols]

Dissection of Spinal Cords for Analysis of Axonal Pathfinding in dsRNA Treated Avian Embryos - http://www.cshprotocols.org/cgi/content/abstract/2006/23/pdb.prot4505

Category: RNAi Technology Protocols: RNAi Avian Embryos Protocols

Protocol describes how to dissect spinal cords of dsRNA-treated avian embryos for analysis of commissural axon guidance. After fixation of the spinal cord with paraformaldehyde, commissural axons are stained with Fast-DiI. - [Read Dissection of Spinal Cords for Analysis of Axonal Pathfinding in dsRNA Treated Avian Embryos]

Dissection of Spinal Cords for Analysis of Axonal Pathfinding in dsRNA-Treated Avian Embryos - http://www.cshprotocols.org/cgi/content/abstract/2006/23/pdb.prot4505

Category: Avian Bird Protocols

Electron Microscopy Protocols - http://www.emlab.ubc.ca/p_em.htm

Category: Molecular Imaging: Electron Microscopy Protocols

Electron Microscopy Protocols ... Chemical Fixation Protocol for Suspension-Cultured Plant Cells for TEM; Standard Glutaraldehyde Fixation for TEM of Animal ...Electron Microscopy Protocols. UBC BioImaging Facility - Bio-Rad Radiance Plus Confocal Microscope - Starting Up, page 1 - [Read Electron Microscopy Protocols]

EM Fixation of C. elegans Embryos Protocol - http://www.aecom.yu.edu/wormem/embryo.htm

Category: C. elegans Protocols: C. elegans Fixing Protocols

Protocol for EM fixation of C. elegans embryos. - [Read EM Fixation of C. elegans Embryos Protocol]

ENLIGHTNING - General Procedure for Gel Autoradiography Enhancement - http://las.perkinelmer.com/applicationssummary/applications/enlightning+protocol.htm

Category: Radioactivity: Autoradiography

Gel Autoradiography Protocols. Drying the gel, exposure, and autoradiography. Protein Fixation and Staining (Optional). ENLIGHTNING - General Procedure for Gel Autoradiography Enhancement. PerkinElmer - [Read ENLIGHTNING - General Procedure for Gel Autoradiography Enhancement]

FISH Protocols for Drosophila - http://www.utoronto.ca/krause/FISH.html

Category: Drosophila Protocols: Drosophila Genetics Protocols

FISH protocols for Drosophila. Includes: RNA Probe Preparation; Embryo Collection and Fixation; Single FISH on Drosophila embryos; Post-Fixation, Hybridization and Post-Hybridization Washes; Development of FISH Signal; Storage, Mounting and Viewing of Samples; Double FISH on Drosophila Embryos; RNA-Protein Double Labeling; FISH on Dissected Tissues. - [Read FISH Protocols for Drosophila]

FIXATION and DNA Staining for Cell Cycle Analysis - http://www.biotech.iastate.edu/facilities/CELLHYB/CCAnalysis.htm

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols

FIXATION and DNA Staining for Cell Cycle Analysis Protocol. This method of DNA staining utilizes ethanol to fix the cells and permeabilize the membrane, which allows the dye (Propidium Iodide) to enter the cells. Propidium Iodide (PI) is a DNA-binding fluorochrome that intercalates in the double-helix. Ribonuclease-A is used to eliminate the staining of double-stranded RNA. - [Read FIXATION and DNA Staining for Cell Cycle Analysis]

Fixation and Fixatives - A great guide - http://home.primus.com.au/royellis/fix.htm

Category: Histology Protocols: Fixation of Cells Tissues Protocol

Fixation is to preserve cells and tissue constituents in as close a life-like state as possible and to allow them to undergo further preparative procedures without change. Fixation arrests autolysis and bacterial decomposition and stabilises the cellular and tissue constituents so that they withstand the subsequent stages of tissue processing. Great detailed guide with protocols. Anthony S-Y Leong. - [Read Fixation and Fixatives - A great guide]

Fixation and Permeabilization of C. elegans Protocol - http://cobweb.dartmouth.edu/cgi-bin/cgiwrap/~ambros/protocol.cgi?id=35

Category: C. elegans Protocols: C. elegans Fixing Protocols

Protocol for fixation and permeabilization of C. elegans. Protocol works for all stages except dauers (which won't open) and hypoclorite-treated eggs (which disintegrate). Luckily, hypochlorite treatment and fixation are by themselves sufficient to open eggs. - [Read Fixation and Permeabilization of C. elegans Protocol]

Fixation and Permeabilization Protocol - http://cobweb.dartmouth.edu/cgi-bin/cgiwrap/~ambros/protocol.cgi?id=35

Category: Immunohistochemistry Protocols: Fixation Protocols

Protocol for fixation and permeabilization. - [Read Fixation and Permeabilization Protocol]

Articles (1-9 of 9)

Paraffin Embedding Protocol

Paraffin Embedding Protocol for molecular profiling. This Paraffin Embedding Protocol describes the processing of the tissues into sections following ethanol fixation. Molecular profiling (MP) is a technique that is used to visualize the global patterns of RNA expression or protein expression in various cell types and disease processes.