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Optimized Protocols for Fluorescent in situ Hybridization in Drosophila Tissues - http://www.utoronto.ca/krause/documents/FISHCHAPTER.pdf

Category: Drosophila Protocols: Drosophila Genetics Protocols

Optimized protocols for fluorescent in situ hybridization in Drosophila tissues. Includes: RNA Probe Preparation; Initial Embryo Fixation; Post-Fixation, Hybridization and post-Hybridization Washes; Development of FISH Signal; Mounting and Viewing of Samples; Double FISH; FISH on Dissected Tissues; RNA-Protein Double-labeling. - [Read Optimized Protocols for Fluorescent in situ Hybridization in Drosophila Tissues]

Preparation of metaphase chromosomes from lymphoblastoid cell lines - http://www.sanger.ac.uk/HGP/methods/cytogenetics/metaphase.shtml

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Preparation of metaphase chromosomes from lymphoblastoid cell lines for FISH analysis. Sanger Institue. - [Read Preparation of metaphase chromosomes from lymphoblastoid cell lines]

Preparation of Paraffin Sections and Frozen Tissue for FISH Protocol - http://www.riedlab.nci.nih.gov/publications/2412.2845_Paraff_FrozTiss_Slide_Pretreat.pdf

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Protocol for the preparation of paraffin sections and frozen tissue for FISH. - [Read Preparation of Paraffin Sections and Frozen Tissue for FISH Protocol]

Preparation of Stock Solutions for FISH Protocol - http://users.ugent.be/~fspelema/neubla/protocol/stock.htm

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Protocol for the preparation of stock solutions for FISH. - [Read Preparation of Stock Solutions for FISH Protocol]

Pretreatment of Chromosome Slides for FISH/CGH/SKY Protocol - http://www.riedlab.nci.nih.gov/publications/Slide%20Pretreatment.pdf

Category: Cytogenetics Protocols

Protocol for pretreatment of chromosome slides for FISH/CGH/SKY. - [Read Pretreatment of Chromosome Slides for FISH/CGH/SKY Protocol]

Pretreatment of Chromosome Slides for FISH/CGH/SKY Protocol - http://www.riedlab.nci.nih.gov/publications/Slide%20Pretreatment.pdf

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Protocol for the pretreatment of chromosome slides for FISH/CGH/SKY. - [Read Pretreatment of Chromosome Slides for FISH/CGH/SKY Protocol]

Pretreatment of Chromosome Slides for FISH/CGH/SKY Protocol - http://www.riedlab.nci.nih.gov/publications/Slide%20Pretreatment.pdf

Category: Cytogenetics Protocols: Spectral Karyotyping SKY Protocols

Protocol for pretreatment of chromosome slides for FISH/CGH/SKY. - [Read Pretreatment of Chromosome Slides for FISH/CGH/SKY Protocol]

Single Colour Fish Analysis Method - http://www.sanger.ac.uk/HGP/methods/cytogenetics/single_colour.shtml

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Single colour detection for biotinylated probes. Sanger Institute - [Read Single Colour Fish Analysis Method]

SKY/FISH of Previously G-Banded Slides Protocol - http://www.riedlab.nci.nih.gov/publications/SKY-FISH%20G_Banded_slides.pdf

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Protocol for SKY/FISH of previously G-banded slides. Includes: G-Band Slide Pretreatment; G-Band Slide Denaturation and Hybridization. - [Read SKY/FISH of Previously G-Banded Slides Protocol]

SKY/FISH of Previously G-Banded Slides Protocol - http://www.riedlab.nci.nih.gov/publications/SKY-FISH%20G_Banded_slides.pdf

Category: Cytogenetics Protocols: Spectral Karyotyping SKY Protocols

Protocol for SKY/FISH of previously G-Banded slides. Includes: G-Band Slide Pretreatment; G-Band Slide Denaturation and Hybridization. - [Read SKY/FISH of Previously G-Banded Slides Protocol]

Slide preparation for FISH and Cytogenetics - http://www.sanger.ac.uk/HGP/methods/cytogenetics/slide_preparation.shtml

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Slide preparation for FISH and Cytogenetics. - [Read Slide preparation for FISH and Cytogenetics]

Small Marker Chromosome Identification in Metaphase and Interphase using Centromeric Multiplex FISH - http://info.med.yale.edu/genetics/ward/tavi/CMFISH.html

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Protocol for small marker chromosome identification in metaphase and interphase using centromeric multiplex FISH (CM-FISH). This approach allows the identification of marker chromosomes and other aneuploides on one single cytogenetic slide, in less than two hours. - [Read Small Marker Chromosome Identification in Metaphase and Interphase using Centromeric Multiplex FISH]

Storage of Chromosome Preparations for FISH Protocol - http://www.riedlab.nci.nih.gov/publications/2415.3738_Storage_Chrom_Prep.pdf

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Protocol for the storage of chromosome preparations for FISH. Includes: Cell Pellet; Chromosome Slide Preparation. - [Read Storage of Chromosome Preparations for FISH Protocol]

Triple Color FISH Technique for Mouse Chromosome Identification Protocol - http://info.med.yale.edu/genetics/ward/tavi/mamgenome.html

Category: Mouse Protocols: Mouse Genetics Protocols

Protocol for triple color FISH technique for mouse chromosome identification. - [Read Triple Color FISH Technique for Mouse Chromosome Identification Protocol]

Whole Mount Fluorescence in Situ Hybridization (FISH) of Repetitive DNA Sequences on Interphase - http://www.roche-applied-science.com/PROD_INF/MANUALS/InSitu/pdf/ISH_220-228.pdf

Category: Plant Biology Protocols: Arabidopsis Genetics Protocols

Protocol for whole mount fluorescence in situ hybridization (FISH) of repetitive DNA sequences on interphase nuclei of the small cruciferous plant Arabidopsis thaliana. Includes: Seed sterilization and germination; Tissue fixation; Labeling of the probe DNA; Pretreatment; In situ hybridization; Pre-absorption of antibodies; Posthybridization washes; Immunocytochemical detection; Direct detection; Indirect detection; Staining and mounting; Fluorescence microscopy. - [Read Whole Mount Fluorescence in Situ Hybridization (FISH) of Repetitive DNA Sequences on Interphase]

Xenopus Fluorescent in situs and FCIS Protocol - http://www.xenbase.org/other/static/methods/FISH.jsp

Category: Xenopus Protocols: Xenopus Genetics Protocols

Protocols for performing wholemount fluorescent in situs. Instructions for two-color FISH and for a combination of fluorescent and colorimetric in situs we have called FCIS. Data can be imaged with a fluorescence stereoscope or a confocal. - [Read Xenopus Fluorescent in situs and FCIS Protocol]

Yeast DNA Isolation Protocol for FISH - http://www.sanger.ac.uk/HGP/methods/cytogenetics/yacs.shtml

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Yeast DNA Isolation Protocol for FISH. Sanger Institute. - [Read Yeast DNA Isolation Protocol for FISH]

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