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A Triple Color FISH Technique for Mouse Chromosome Identification - http://info.med.yale.edu/genetics/ward/tavi/mamgenome.html

Category: BACs Bacterial Artificial Chromosome Protocols

Describe a set of BACs that map close to the centromeric and distal chromosomal ends for each mouse chromosome. - [Read A Triple Color FISH Technique for Mouse Chromosome Identification]

Additional slide pre-treatment for mapping smaller insert clones - http://www.sanger.ac.uk/HGP/methods/cytogenetics/additional_treatment.shtml

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Additional slide pre-treatment for mapping smaller insert clones for FISH. Sanger Institute. - [Read Additional slide pre-treatment for mapping smaller insert clones]

Application of Direct Current Electric Fields to Cells and Tissues in vitro - http://www.natureprotocols.com/2006/12/12/application_of_direct_current.php

Category: Cell Biology and Cell Culture

Protocol applies EFs to cells in vitro but has been modified and to use electrotactic chambers to accommodate cells growing in planar culture or in three-dimensional (3D) gels, en bloc tissue cultures in 3D and possible small embryos, such as that from frog and zebra fish. The EF is applied to the cells or tissues cultured in a customer designed electrotactic chamber via agar salt bridges, Steinbergâ€™s solution and Ag/AgCl electrodes. - [Read Application of Direct Current Electric Fields to Cells and Tissues in vitro]

BAC-FISH Protocol - http://www.nhm.ac.uk/hosted_sites/schisto/protocols/BAC_FISH.html

Category: BACs Bacterial Artificial Chromosome Protocols

Protocol uses the BIOPRIME reaction kit from GibcoBRL to prepare biotin-labelled BAC DNA which is detected using FITC-Avidin (Vector Labs, DCS grade). Reagents from other manufacturers may work equally well but have not been tested. Includes: Labeling of BAC clones; Ethanol precipitation; Hybridization; Post-hybridisation treatment / detection. - [Read BAC-FISH Protocol]

Bacterial clone DNA Protocol for FISH - http://www.sanger.ac.uk/HGP/methods/cytogenetics/DNAprep.shtml

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Bacterial clone DNA Protocol for FISH. - [Read Bacterial clone DNA Protocol for FISH]

BioPrime Biotin Labeling (FISH probe) Protocol - http://www.jax.org/staff/mills/protocols/bioprime_biotin_labeling.pdf

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Protocol for BioPrime biotin labeling (FISH probe). - [Read BioPrime Biotin Labeling (FISH probe) Protocol]

Chromosome Preparation and Fluorescence In-Situ Hybridization (FISH) Protocol - http://www.scienceboard.net/resources/protocols.asp?action=article&protocol_id=18

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Protocol for chromosome preparation and Fluorescence in-situ hybridization (FISH). Chromosome preparation and Fluorescence in-situ hybridization (FISH). - [Read Chromosome Preparation and Fluorescence In-Situ Hybridization (FISH) Protocol]

Color Changing Karyotyping (CCK) Protocol: An M-FISH/SKY Alternative - http://info.med.yale.edu/genetics/ward/tavi/CCKmethod.html

Category: Cytogenetics Protocols

Protocol for color changing karytoping (CCK): an M-FISH/SKY alternative. Includes: Slide preparation and hybridization; Probes, dyes and antibodies; Probe detection and image capture; Image processing. - [Read Color Changing Karyotyping (CCK) Protocol: An M-FISH/SKY Alternative]

Cryptic Translocation Identification in Human and Mouse Using Several Telomeric Multiplex FISH - http://info.med.yale.edu/genetics/ward/tavi/TMFISH.html

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Protocol for cryptic translocation identification in human and mouse using several telomeric multiplex FISH (TM-FISH) strategies. - [Read Cryptic Translocation Identification in Human and Mouse Using Several Telomeric Multiplex FISH]

Detection FISH Protocol - http://www.riedlab.nci.nih.gov/publications/FISH%20Detection.pdf

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Protocol for Detection (FISH). TRITC, or Avidin Cy-5. For the probes labeled with digoxigenin, we usually first incubate with mouse-anti-digoxigenin, followed by incubation with sheep anti-mouse Cy5.5, or other fluorochrome conjugated antibodies. - [Read Detection FISH Protocol]

DNA Extraction from Fish Fins Protocol - http://genome-lab.ucdavis.edu/Protocols/dna_extraction_from_fish_fins.htm

Category: Nucleic Acid Purification Protocols: DNA Isolation Protocols

Protocol for DNA extraction from fish fins. - [Read DNA Extraction from Fish Fins Protocol]

DNA FIBERS FISH - http://www.sanger.ac.uk/HGP/methods/cytogenetics/fiber_fish.shtml

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

DNA FIBERS FISH Protocol and Methods. Sanger Institute. - [Read DNA FIBERS FISH]

DNA Precipitation and Hybridization FISH Protocol - http://www.riedlab.nci.nih.gov/publications/DNA%20Precip%20&%20Hybrid%20FISH.pdf

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Protocol for DNA precipitation and hybridization (FISH). Includes: Precipitation; Slide Denaturation and Hybridization. - [Read DNA Precipitation and Hybridization FISH Protocol]

FISH Guide and Troubleshooting - http://info.med.yale.edu/genetics/ward/tavi/FISHguide.html

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

FISH Guide and Troubleshooting. Includes: Cytogenetic techniques; Slide preparation; When do chromosomes spread; Cell suspensions; G-banding; Cell fixatives; Slide preparation factors. - [Read FISH Guide and Troubleshooting]

FISH Hybridization Protocol - http://www.sanger.ac.uk/HGP/methods/cytogenetics/hybridisation.shtml

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

FISH Hybridization Protocol. Sanger Institute. - [Read FISH Hybridization Protocol]

FISH Protocols for Drosophila - http://www.utoronto.ca/krause/FISH.html

Category: Drosophila Protocols: Drosophila Genetics Protocols

FISH protocols for Drosophila. Includes: RNA Probe Preparation; Embryo Collection and Fixation; Single FISH on Drosophila embryos; Post-Fixation, Hybridization and Post-Hybridization Washes; Development of FISH Signal; Storage, Mounting and Viewing of Samples; Double FISH on Drosophila Embryos; RNA-Protein Double Labeling; FISH on Dissected Tissues. - [Read FISH Protocols for Drosophila]

Fluorescence In Situ Hybridization (FISH) for DNA Replication Origins Protocol - http://www.natureprotocols.com/2006/10/27/fluorescence_in_situ_hybridiza.php

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Protocol for fluorescence in situ hybridization (FISH) for DNA replication origins. Fluorescent in situ hybridization (FISH) is a molecular cytogenetic technique used for the detection of specific chromosomal rearrangements and applicable to many different specimen types. FISH is widely used for several diagnostic applications. - [Read Fluorescence In Situ Hybridization (FISH) for DNA Replication Origins Protocol]

Fluorescence In Situ Hybridization (FISH) on Human Chromosomes and Interphase Nuclei Protocol - http://users.ugent.be/~fspelema/neubla/protocol/fish.htm

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Protocol for fluorescence in situ hybridization (FISH) on human chromosomes and interphase nuclei. Includes: Plasmid probes; COSMID-, YAC- and LIBRARY PROBES; Probe detection. - [Read Fluorescence In Situ Hybridization (FISH) on Human Chromosomes and Interphase Nuclei Protocol]

Fluorescence in situ Hybridization Protocol - http://www.ciwemb.edu/labs/koshland/Protocols/MICROSCOPY/fish.html

Category: Yeast Protocols: Yeast Nucleic Acid Protocols

Protocol for fluorescence in situ hybridization. - [Read Fluorescence in situ Hybridization Protocol]

In Situ Hybridization to Human Metaphase Chromosomes using DIG-, Biotin- or Fluorochrome-Labeled DNA - http://www.roche-applied-science.com/PROD_INF/MANUALS/InSitu/pdf/ISH_78-94.pdf

Category: Cytogenetics Protocols: DIG Biotin Labeled Probe Protocols

Protocol for In situ hybridization to human metaphase chromosomes using DIG-, biotin-, or fluorochrome-labeled DNA probes and detection with fluorochrome conjugates. Includes: Pretreatment of metaphase spreads on slides; Denaturation and hybridization; Single color fluorescent detection with immunological amplification; Multicolor fluorescence in situ hybridization (Multicolor FISH); Results obtained with human metaphase chromosome spreads. - [Read In Situ Hybridization to Human Metaphase Chromosomes using DIG-, Biotin- or Fluorochrome-Labeled DNA]

Interphase nuclei - http://www.sanger.ac.uk/HGP/methods/cytogenetics/interphase_nuclei.shtml

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Interphase nuclei preparation for FISH. Sanger Institute. - [Read Interphase nuclei]

Inverse PCR for PAC-End Sequencing Protocol - http://www.genetics.wustl.edu/fish_lab/frank/cgi-bin/fish/prot2.html

Category: PCR Protocols: Inverse PCR Protocols

Protocol for inverse PCR for PAC-end sequencing. - [Read Inverse PCR for PAC-End Sequencing Protocol]

Isolation of Interphase Nuclei from Paraffin Section for FISH, Hedley Technique - http://www.riedlab.nci.nih.gov/publications/2408.2950_Isolat_Interph_Nuc4Hed204.pdf

Category: Cell Organelle Protocols: Nucleus Protocols

Protocol for the isolation of interphase nuclei from Paraffin Section for FISH using the Hedley Technique. - [Read Isolation of Interphase Nuclei from Paraffin Section for FISH, Hedley Technique]

Nick Translation FISH Protocol - http://www.riedlab.nci.nih.gov/publications/FISH%20Nick%20Translation.pdf

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Protocol for Nick Translation FISH. - [Read Nick Translation FISH Protocol]