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12-well Chemotaxis Chamber Protocol - http://www.neuroprobe.com/protocols/AA12.html

12-well Chemotaxis Chamber Protocol. Preparing the Chamber, Preparing and Adding Responding Cells, Removing, Wiping and Staining the Filter. Neuroprobe - [Read 12-well Chemotaxis Chamber Protocol]

Activated MAP Kinase Protocols Stratagene PDF - http://www.stratagene.com/manuals/206110.pdf

Category: Signalling Signal Transduction Protocols: MAPK Protocols

MAPK Protocols. Resolving Phosphorylated MAPK Proteins by SDSâ€“PAGE, Filter Binding Assay for activated MAPK Î³-32P incorporation of MAPK. Stratagene - [Read Activated MAP Kinase Protocols Stratagene PDF]

Analysis Of DNA Fragmentation Using The JAM Assay (Subscription Required) - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4432

Category: Cell Biology and Cell Culture: Apoptosis Protocols: Apoptosis Analysis

Analysis of DNA Fragmentation Using the JAM Assay. By Shailaja Kasibhatla et al., The JAM assay is based on labeling nuclear DNA of cycling cells with [3H]thymidine and harvesting samples on glass fiber filters. Apoptosis will generate DNA fragments small enough to pass through the glass fiber filter, resulting in decreased radioactivity of the particular sample. Cell-mediated cytotoxicity or cell killing mediated by cytotoxic T lymphocytes (CTL) can also be measured by this technique. - [Read Analysis Of DNA Fragmentation Using The JAM Assay (Subscription Required)]

ß - GAL Filter Assay Protocol - http://www.fhcrc.org/science/labs/breeden/Methods/b-GAL_filterassay.html

Category: Yeast Protocols: Yeast Genetics Protocols: B-Galactosidase Assay Protocols

Protocol for ß - GAL filter assay. - [Read ß - GAL Filter Assay Protocol]

B-GAL Filter Assay Protocol - http://www.fhcrc.org/science/labs/breeden/Methods/b-GAL_filterassay.html

Category: Reporter Gene Assays: B-Galactosidase Assay Protocols

Protocol for B-Gal filter assay. Includes Z buffer and X-Gal solution. - [Read B-GAL Filter Assay Protocol]

Elispot plates column - http://www.zellnet.com/elispotplatescolumn/

Category: ELISA Protocols: ELISpot Protocols

Tips for selecting the â€œrightâ€ ELISPOT filter plate and making the best protocol choices. Alan J. Weiss ZellNet - [Read Elispot plates column]

Hybridization of Bacteriophage DNA on Filters Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3983

Category: Viral Manipulation Protocols: Phage Protocols

Using hybridization, it is possible to identify a single recombinant that carries the desired target sequence on a filter that carries the imprint of 15,000 or more plaques. - [Read Hybridization of Bacteriophage DNA on Filters Protocol]

Immunoblotting: Semi-Dry Electrophoretic Transfer of Proteins from Gels to Membranes Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/16/pdb.prot4301

Category: Western Blot Protocols: General Western Blot Methods Protocols

The transfer of proteins from a Tris/glycine SDS-polyacrylamide gel to a membrane using a semi-dry method is achieved by placing the gel next to a piece of nitrocellulose filter. This sandwich is placed directly between two plate electrodes, and the proteins are then transferred from the gel onto the filter. - [Read Immunoblotting: Semi-Dry Electrophoretic Transfer of Proteins from Gels to Membranes Protocol]

Immunoblotting: Submerged Electrophoretic Transfer of Proteins from Gels to Membranes Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/16/pdb.prot4302

Category: Western Blot Protocols: General Western Blot Methods Protocols

Transfer of proteins from a Tris/glycine SDS-polyacrylamide gel to a membrane using the submerged method is achieved by placing the gel next to a piece of nitrocellulose filter, submerging this sandwich in a large volume of transfer buffer in a transfer tank, and running current from one side of the transfer tank to another. The proteins are then eluted by transferring them from the gel onto the filter. - [Read Immunoblotting: Submerged Electrophoretic Transfer of Proteins from Gels to Membranes Protocol]

Introduction to Fluorescence Microscopy - http://www.microscopyu.com/articles/fluorescence/fluorescenceintro.html

Category: Microscopy Protocols: Fluorescence Microscopy Protocols

Article presents an introduction to fluorescence microscopy. Includes: Fundamentals of Excitation and Emission; Stokes' Shift; Fading, Quenching, and Photobleaching; Fluorescence Light Sources; Filter Terminology; The Fluorescence Light Budget; Detecting Single Molecules. - [Read Introduction to Fluorescence Microscopy]

Lysing Colonies and Binding of DNA to Filters Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3938

Category: Bacterial Protocols

Alkali is used to liberate DNA from bacterial colonies on nitrocellulose or nylon filters. The DNA is then fixed to the filter by UV-cross-linking or baking under vacuum. - [Read Lysing Colonies and Binding of DNA to Filters Protocol]

Quantitative GUS Activity Assay of Plant Extracts - http://www.cshprotocols.org/cgi/content/abstract/2007/3/pdb.prot4690

Category: Plant Biology Protocols: Plant Proteins, Peptides and Antigens

Using excitation at 365 nm and measuring emission at 455 nm, the amount of 4-MU produced can be quantified. Under these conditions, background fluorescence from the substrate is negligible, especially if the appropriate filter is selected. - [Read Quantitative GUS Activity Assay of Plant Extracts]

Screening Bacterial Colonies by Hybridization: Small Numbers Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3935

Category: Bacterial Protocols

Protocol used to screen a small number of bacterial colonies (<200) that are dispersed over several agar plates and are to be screened by hybridization to the same radiolabeled probe. The colonies are gridded onto a master plate and onto a nitrocellulose or nylon filter laid on the surface of a second agar plate. - [Read Screening Bacterial Colonies by Hybridization: Small Numbers Protocol]

Articles (1-6 of 6)

Single Stranded Plasmid DNA Isolation Protocol

A Single Stranded Plasmid DNA Isolation Protocol describing the production and isolation of single-stranded DNA (ssDNA) using bacteriophagemid-containing bacteria and helper phage. Infection of the host cells with helper phage allows for packaging of ssDNA into bacteriophage. The ssDNA can then be isolated from phage particles.

Genomic DNA Labeling of Microarrays Protocol

DNA microarrays are an ordered arrangement of DNA molecules complementary to genes of interest that are "spotted" by robotic equipment onto a glass slide substrate. The expression of genes in cells can be monitored with microarrays by preparing cDNA from the mRNA of cells of interest and measuring the hybridization to the microarray. This protocol describes the labeling of genomic DNA for use as a probe for hybridization to the cDNA spotted on the array.

Drosophila Cell Transfection Protocol

A simple Drosophila tissue culture cell transfection method.

Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA

This Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA protocol describes the production of probes labeled with the fluorescent dyes, Cy3 and Cy5, following the synthesis of cDNA from human mRNA and the hybridization of the probes to DNA microarrays.

Immobilized Antigen Phage Antibody Selection Protocol

A protocol for the selection of Phage Antibodies using Immobilized Antigen. This method describes the selection of antibodies from bacteriophage antibody libraries that recognize a specific antigen. The phage display library of antibody-displaying phage particles is exposed to antigen attached to a solid substrate (Nunc Immuno™ tubes). The phage particles with affinity for antigen bind to the immobilized antigen and are selected from the library of phage expressing antibodies.

Picking Transfected ES Cell Clones Protocol

This protocol a protocol on how to generate transfected embryonic stem (ES) cell clones. The previous protocol in this series is the Protocol for Electroporation of ES cells. The next protocol in the series is the Protocol on Disaggregation, Expansion, and Freezing of Transfected ES Clones.

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