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Category: Microinjection Protocols: Microinjection of DNA Protocols
For microinjection into cells, high-quality plasmid DNA is purified using standard procedures. The resulting preparation is then delivered into the microinjection needle by either a back-filling or a forward-filling approach. - [Read DNA Preparation and Needle Loading for Gene Delivery by Microinjection Protocol]
Category: C. elegans Protocols: C. elegans Staining Protocols
Protocol for double dye filling (using DiO and di-4 ANEPPS). - [Read Double Dye Filling (using DiO and di-4 ANEPPS) Protocol]
Protocol for dye filling to stain amphid and phasmid neurons. - [Read Dye Filling to Stain Amphid and Phasmid Neurons Protocol]
Category: Neuroscience Protocols: Neuronal Stains Protocols
Protocol for nematode dye filling. Includes: Thumb plate; Microfuge tube; 15 ml tube; Viewing: Big Scope and Dissecting Scope. - [Read Nematode Dye Filling Protocol]
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