ficoll Protocols

Category: Cell Organelle Protocols: Nucleus Protocols

The key step is the lysis which solubilizes centrosomes away from nuclei by very low ionic strength lysis after treatment of cells with nocodazole and cytochalasin B. The released centrosomes are then centrifuged onto a Ficoll cushion (to avoid pelleting) and the interface between the lysate and the Ficoll is collected and the centrosomes are concentrated on a sucrose gradient. Fractions are assayed by spindown and double IF with 5051 serum and anti-tubulin and the pooled fractions are frozen... - [Read CHO Centrosome Prep Protocol]

Category: Immunology: Mononuclear Cell Isolation Protocols: B Lymphocyte Isolation Protocols

Protocol describes a method for isolation and stimulation of lymphocytes. Leukocyte-rich plasma is collected from whole blood and then centrifuged through Ficoll-Hypaque. The collected cells are resuspended in growth medium containing various mitogens to stimulate growth. - [Read Lymphocyte Isolation and Culture Protocol]