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Immunodepletion Protocol - http://www.fgsc.net/neurosporaprotocols/Immunodepletion%20final.pdf

Category: Protein Protocols

Protocol for studying quantitative relationships of proteins in a complex. - [Read Immunodepletion Protocol]

In Vitro System to Analyze Light Signal Transduction in Neurospora crassa - http://www.fgsc.net/neurosporaprotocols/How%20to%20analyze%20light%20signal%20transduction%20in%20vitro.pdf

Category: Fungi Protocols: Neurospora Protocols

Procedure for an in vitro system to analyze light signal transduction in Neurospora crassa - [Read In Vitro System to Analyze Light Signal Transduction in Neurospora crassa]

Lipofectin Increases the Efficiency of DNA-Mediated Transformation of Neurospora Crassa - http://www.fgsc.net/fgn38/selitren.html

Category: Fungi Protocols: Neurospora Protocols

Lipofectin increases the efficiency of DNA-mediated transformation of Neurospora crassa. - [Read Lipofectin Increases the Efficiency of DNA-Mediated Transformation of Neurospora Crassa]

Media for Culture of Aspergillus nidulans Protocol - http://www.fgsc.net/methods/anidmed.html

Category: Cell Biology and Cell Culture: Cell Culture Growth Media: Yeast Cell Culture Growth Media Protocols

Protocol for media for culture of Aspergillus nidulans. Includes minimal and complete media. - [Read Media for Culture of Aspergillus nidulans Protocol]

Media for Culture of Aspergillus nidulans Protocol - http://www.fgsc.net/methods/anidmed.html

Category: Fungi Protocols: Aspergillus Protocols

Protocol for media for culture of Aspergillus nidulans. Includes: Minimal medium; Complete media. - [Read Media for Culture of Aspergillus nidulans Protocol]

Method to Analyze Meiotic Segregation Patterns in Coprinus cinereus Using PCR - http://www.fgsc.net/fgn/freedman.html

Category: Fungi Protocols: Fungi Genetics Protocols

Quick and reliable method to analyze meiotic segregation patterns in Coprinus cinereus using the polymerase chain reaction. The advantages of this method include: 1. The tissue is grown and lyophilized in the same tube, which facilitates the simultaneous analysis of many segregants. 2. Only one extraction step is necessary. 3. The markers are scored by gel electrophoresis, thereby bypassing Southern analysis. - [Read Method to Analyze Meiotic Segregation Patterns in Coprinus cinereus Using PCR]

Neurospora Methods Protocols - http://www.fgsc.net/methods/stanford.html

Category: Fungi Protocols: Neurospora Protocols

Protocols for Neurospora methods. Includes: Standard strains; Crosses; Minimal medium; Color coding of media; Agar substrate for manipulation and isolation; Stock solutions of supplements; Mating-type tests; Preservation of stocks by silica gel; Cleaning of glassware; Control of mites. - [Read Neurospora Methods Protocols]

Northern blot Protocol for the Detection of RNA in Neurospora - http://www.fgsc.net/neurosporaprotocols/Northern%20edited%20version.pdf

Category: Fungi Protocols: Neurospora Protocols

Northern blot protocol for the detection of RNA in Neurospora. Includes: Extract RNA from tissue powder; Electropheresis and transfer RNA; Preparing the membrane for probing and preparation of a riboprob.; Hybridization. - [Read Northern blot Protocol for the Detection of RNA in Neurospora]

Preparing and Transforming Spheroplasts of Neurospora crassa Protocol - http://www.fgsc.net/neurosporaprotocols/How%20to%20Prepare%20Spheroplasts.pdf

Category: Fungi Protocols: Neurospora Protocols

Procedure for preparing and transforming spheroplasts of Neurospora crassa. The procedure is designed to produce a large number of spheroplasts to be stored frozen at -80°. - [Read Preparing and Transforming Spheroplasts of Neurospora crassa Protocol]

Preparing Vacuolar Membranes And Other Membrane Fractions from Neurospora Crassa Protocol - http://www.fgsc.net/neurosporaprotocols/VM%20vacuole%20procedure.pdf

Category: Fungi Protocols: Neurospora Protocols

Protocol for Preparing Vacuolar Membranes And Other Membrane Fractions from Neurospora Crassa (4 liter prep, easily scaled up to 10, 12, 24, or 30 l). - [Read Preparing Vacuolar Membranes And Other Membrane Fractions from Neurospora Crassa Protocol]

Protocol for Bird Medium: Alternative to Vogel Medium - http://www.fgsc.net/fgn51/fgn51metz.html

Category: Fungi Protocols

Protocol for bird medium. This medium was designed to circumvent some problems that arise in the use of Medium N. - [Read Protocol for Bird Medium: Alternative to Vogel Medium]

Protocol for Immunoprecipitation - http://www.fgsc.net/neurosporaprotocols/Immunoprecipitation%20final.pdf

Category: Protein Protocols: Immunoprecipitation Protocols

Protocol for immunoprecipitation. - [Read Protocol for Immunoprecipitation]

Protocol Guide for the N. crassa Yeast Artificial Chromosome Library - http://www.fgsc.net/fgn41/yac1.html

Category: Fungi Protocols: Neurospora Protocols

Protocol guide for the N. crassa yeast artificial chromosome library. Includes: Chromosome Walking; Hybridization screening of the YAC library; YAC restriction mapping and contig building; Preparation of chromosomal DNA plugs of YAC clones; Partial restriction enzyme digestion of YAC DNA plugs; Using CHEF gel analysis to resolve YAC clones; Southern Hybridization; Isolation of terminal restriction fragments from cloned DNA inserts in YAC clones; etc. - [Read Protocol Guide for the N. crassa Yeast Artificial Chromosome Library]

Rapid Method for Making Large Numbers of Crosses Protocol - http://www.fgsc.net/neurosporaprotocols/Rapid__crossesDE.pdf

Category: Fungi Protocols: Neurospora Protocols

Protocol for a rapid method for making large numbers of crosses. - [Read Rapid Method for Making Large Numbers of Crosses Protocol]

Small Scale DNA Preps for Neurospora crassa Protocol - http://www.fgsc.net/fgn/irelan.html

Category: Fungi Protocols: Neurospora Protocols

DNA isolation method yields an average of 0.6 micrograms of genomic DNA that is suitable for Southern analysis or PCR. Starting with fresh mycelium, 20 to 40 samples can be processed in approximately two hours. Better yields (about 5 micrograms) may be obtained by suspending approximately 100 microliters of ground lyophilized mycelium in 500 microliters of isolation buffer and following the protocol. - [Read Small Scale DNA Preps for Neurospora crassa Protocol]

Toxicity of Antibiotics and Other Drugs to Neurospora - http://www.fgsc.net/methods/fgn21tox.html

Category: Fungi Protocols: Neurospora Protocols

Information and tips on the toxicity of antibiotics and other drugs to Neurospora. - [Read Toxicity of Antibiotics and Other Drugs to Neurospora]

Transformation of Magnaporthe grisea to Phosphinothricin Resistance Protocol - http://www.fgsc.net/fgn42/leung.html

Category: Fungi Protocols: Fungi Genetics Protocols

Transformation of Magnaporthe grisea to phosphinothricin resistance using the bar gene from Streptomyces hygroscopicus. - [Read Transformation of Magnaporthe grisea to Phosphinothricin Resistance Protocol]

Ultra-Fast Method of DNA Extraction from Neurospora Protocol - http://www.fgsc.net/fgn/camb.html

Category: Fungi Protocols: Neurospora Protocols

Simple method for growing Neurospora and for isolation of DNA that may be performed in two days from start to finish. The growth of mycelia in Petri plates eliminates the need for large numbers of flasks when growing many cultures for DNA isolation. - [Read Ultra-Fast Method of DNA Extraction from Neurospora Protocol]

Vogel's Minimal Media Protocol - http://www.fgsc.net/methods/vogels.html

Category: Cell Biology and Cell Culture: Cell Culture Growth Media: Yeast Cell Culture Growth Media Protocols

Vogel's 50X salts as given in the Microbial Genetics Bulletin 13:42-43, 1956. In 750 ml. distilled water, dissolve successively with stirring at room temperature. - [Read Vogel's Minimal Media Protocol]

Vogels Medium N (Minimal) Protocol - http://www.fgsc.net/methods/vogels.html

Category: Fungi Protocols

Protocol for Vogels medium N (minimal). - [Read Vogels Medium N (Minimal) Protocol]

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