fgsc Protocols

Category: DNA Protocols: EMSA DNA-Protein Protocols

A non-radioactive electrophoretic mobility shift assay based on the digoxigenin detection system had been developed and applied to the analysis of the interaction between the heat shock transcription factor and the heat shock element of N. crassa. (Bioch - [Read A non-radioactive electrophoretic mobility shift assay for the detection of heat shock element (HSE)]

Category: Fungi Protocols: Aspergillus Protocols

Protocol for the rapid method for isolation of total nucleic acids from Aspergillus nidulans. - [Read A Rapid Method for Isolation of Total Nucleic Acids from Aspergillus nidulans Protocol]

Category: Fungi Protocols: Fungi Genetics Protocols

Protocol describes a safe and convenient method of extracting DNA from Coccidioides immitis fungi in which the culture is killed by steaming, allowing removal from the containment facilities, as soon as possible. The method was first developed with the non-pathogen Neurospora crassa, has worked well for both C. immitis and H. capsulatum, and should be useful for extracting DNA from any pathogenic fungus. - [Read A Safe Method of Extracting DNA from Coccidioides immitis Protocol]

Category: Fungi Protocols: Aspergillus Protocols

Compilation of A. nidulans mutants listing names, affected enzymes (or gene functions), linkage assignments, properties of the mutants, and names of corresponding loci in N. crassa. - [Read Aspergillus nidulans Mutants]

Category: Fungi Protocols: Fungi Genetics Protocols

PCR protocol which uses M. grisea conidia directly for PCR analysis without extraction of DNA. - [Read Assessment of Magnaporthe grisea Mating Type by Spore PCR]

Category: Cell Biology and Cell Culture: Cell Culture Growth Media: Yeast Cell Culture Growth Media Protocols

Protocol for bird Medium: an alternative to Vogel Medium. - [Read Bird Medium Protocol: An Alternative to Vogel Medium]

Category: Fungi Protocols: Neurospora Protocols

Protocol for cryofixation and freeze substitution for ultrastructural studies of Neurospora crassa hyphae. - [Read Cryofixation and Freeze Substitution for Ultrastructural Studies of Neurospora crassa hyphae]

Category: Fungi Protocols: Aspergillus Protocols

Compendium of protocols for using Aspergillus nidulans in genetic, molecular, and cell biological investigations, originally written for members of my research group. It also summarizes our common growth media and nutritional supplements, many of which originally appeared elsewhere but now are difficult to locate. Includes: Growth and storage of Aspergillus nidulans conidia; Nutritional supplements for our common auxotrophies; Double mutants; Mitotic mapping - assigning genes to chromosomes; etc - [Read Fundamentals of Growth, Storage, Genetics and Microscopy of Aspergillus nidulans Protocols]

Category: Fungi Protocols: Neurospora Protocols

Protocol for generation of transformable spheroplasts from mycelia, macroconidia, microconidia and germinating ascospores of Neurospora crassa. - [Read Generation of Transformable Spheroplasts Protocol]

Category: Fungi Protocols: Neurospora Protocols

Hints and precautions for the care, feeding and breeding of Neurospora. Includes: Crossing; Tips on handling ascospoes General Laboratory Practices; Stockkeeping; Mutagenesis and enrichment; Tips for encouraging colonial growth; Solutions and media; Handling Heterokaryons. - [Read Hints and Precautions for the Care, Feeding and Breeding of Neurospora]

Category: Fungi Protocols: Neurospora Protocols

Information and tips on how and where to obtain information on Neurospora. - [Read How and Where to Obtain Information on Neurospora]

Category: Fungi Protocols: Neurospora Protocols

Information and tips on how to activate ascospores and optimize the recovery of progeny. - [Read How to Activate Ascospores and Optimize the Recovery of Progeny]

Category: Fungi Protocols: Neurospora Protocols

Information and tips on how to amplify DNA from macroconidia. - [Read How to Amplify DNA from Macroconidia]

Category: Fungi Protocols: Neurospora Protocols

Information and tips on how to assay light responses in Neurospora. - [Read How to Assay Light Responses in Neurospora]

Category: Fungi Protocols: Neurospora Protocols

Information and tips on How to avoid contamination by airborne conidia. - [Read How to Avoid Contamination by Airborne Conidia]

Category: Fungi Protocols: Neurospora Protocols

Information and tips on how to avoid pitfalls and keep out of trouble in the Neurospora lab. Includes: Culture, incubation, storage, and disposal; Stock preservation; Spore and colony counts; Making crosses and recovering progeny; Use of heterokaryons for allelism tests; Use of unordered asci to estimate the relation of translocation breakpoints to centromeres. - [Read How to Avoid Pitfalls and Keep Out of Trouble in the Neurospora Lab]

Category: Fungi Protocols: Neurospora Protocols

Information and tips on how to certify that Neurospora is nonhazardous. - [Read How to Certify that Neurospora is Nonhazardous]

Category: Fungi Protocols: Neurospora Protocols

Information and tips on how to choose and prepare media. - [Read How to Choose and Prepare Media]

Category: Fungi Protocols: Neurospora Protocols

Information and tips on how to choose wild type laboratory strains of N. crassa for reference and for special uses. - [Read How to Choose Wild Type Laboratory Strains of N. crassa for Reference and for Special Uses]

Category: Fungi Protocols: Neurospora Protocols

Information on how to color-code media. - [Read How to Color-Code Media]

Category: Fungi Protocols: Neurospora Protocols

Information and tips on how to convert wild-type spreading growth to colonial. - [Read How to Convert Wild-Type Spreading Growth to Colonial]

Category: Fungi Protocols: Neurospora Protocols

Information and tips on how to create gene knockouts in Neurospora. - [Read How to Create Gene Knockouts in Neurospora]

Category: Protein Protocols: Immunoprecipitation Protocols

Information and tips on how to cross-link proteins. - [Read How to Cross-Link Proteins]

Category: Fungi Protocols: Neurospora Protocols

Information and tips on how to deal with mite infestations and minimize their occurrence. - [Read How to Deal with Mite Infestations and Minimize their Occurrence]

Category: Fungi Protocols: Neurospora Protocols

Information and tips on how to demonstrate barrage in Neurospora. - [Read How to Demonstrate Barrage in Neurospora]