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Genetically-Engineered Mouse Models - http://www.bcm.edu/dtmc/

Category: Microinjection Protocols: Microinjection of DNA Protocols

Microinjection Services – mouse ES cells into blastocysts and DNA into pronucleus of fertilized eggs. - [Read Genetically-Engineered Mouse Models]

Microinjection of DNA into Pronucleus of Fertilized Eggs Protocol - http://www.bcm.edu/dtmc/?pmid=7785

Category: Microinjection Protocols: Microinjection of DNA Protocols

Protocol for microinjection of DNA into pronucleus of fertilized eggs. - [Read Microinjection of DNA into Pronucleus of Fertilized Eggs Protocol]

Microinjection of dsRNA into Mouse Oocytes and Early Embryos Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/21/pdb.prot4511

Category: RNAi Technology Protocols: RNAi Mouse Embryos and Oocytes Protocols

Protocol describes how to introduce a double-stranded RNA (dsRNA) of choice into mouse oocytes or fertilized one-cell embryos by microinjection. For collection of mouse oocytes and early embryos, see Collection of Mouse Oocytes for RNAi and Collection of Early Mouse Embryos for RNAi. - [Read Microinjection of dsRNA into Mouse Oocytes and Early Embryos Protocol]

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In Vitro Translated Xenopus Mos Kinase Assay Protocol

In Vitro Translated Xenopus Mos Kinase Assay Protocol. In response to progesterone, immature Xenopus oocytes mature to eggs that can be fertilized. The Mos protein kinase is essential for oocyte maturation, most likely due to its ability to activate the MAP kinase cascade. This MAP kinase cascade eventually leads to the activation of Cdc2/cyclin B and entry into M phase. In this protocol, tagged Mos kinase is translated in vitro, immunopurified, and used in a kinase assay.