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Antibody Purification Protocol - http://iacf.bsd.uchicago.edu/Monoclonal_Antibody_Facility/AntibodyPurificationProtocol.htm

Category: Protein Protocols: Antibody Purification Protocols

Antibody Purification Protocol. Monoclonal Antibody Facility - [Read Antibody Purification Protocol]

Basic ELISA Method - http://iacf.bsd.uchicago.edu/Monoclonal_Antibody_Facility/BasicELISAProtocol.htm

Category: ELISA Protocols

Basic ELISA Method. All the steps for the basic ELISA protocol. Immunology Applications Core Facility, Univ. Chicago. - [Read Basic ELISA Method]

Basic Method for Direct Immunofluorescence Labeling - http://www.biotech.iastate.edu/facilities/CELLHYB/Labeling.htm

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols

Basic Method for Direct Immunofluorescence Labeling. Iowa State University, Flow Cytometry Facility. - [Read Basic Method for Direct Immunofluorescence Labeling]

Cell Culture Guidelines - http://iacf.bsd.uchicago.edu/Monoclonal_Antibody_Facility/CellCultureGuidelines.htm

Category: Cell Biology and Cell Culture: Troubleshooting Cell Culture Problems

Immunology Applications Core Facility, Univ. Chicago - [Read Cell Culture Guidelines]

Cell Thawing and cell line Freezing Protocol - http://iacf.bsd.uchicago.edu/Monoclonal_Antibody_Facility/FreezeThawProtocol.htm

Category: Cell Biology and Cell Culture: Cell Culture Techniques: Cell Line Preservation Freezing

Method for freezing and thawing cells. Immunology Applications Core Facility. U. Chicago - [Read Cell Thawing and cell line Freezing Protocol]

Electron Microscopy Protocols - http://www.emlab.ubc.ca/p_em.htm

Category: Molecular Imaging: Electron Microscopy Protocols

Electron Microscopy Protocols ... Chemical Fixation Protocol for Suspension-Cultured Plant Cells for TEM; Standard Glutaraldehyde Fixation for TEM of Animal ...Electron Microscopy Protocols. UBC BioImaging Facility - Bio-Rad Radiance Plus Confocal Microscope - Starting Up, page 1 - [Read Electron Microscopy Protocols]

ES cell culture protocols (2005 update) PDF Excellent - http://www.mshri.on.ca/cores/ESfacility/ESculture.pdf

Category: Cell Biology and Cell Culture: ES Stem Cell Biology and Culture: ES Cell Growth Media and Requirements

ES cell culture protocols (2005 update) PDF Excellent. ES cell facility, Samuel Lunenfeld Research Institute, Mount Sinai Hospital. - [Read ES cell culture protocols (2005 update) PDF Excellent]

ES mouse cell stem cell Culture - http://imgen.bcm.tmc.edu/dtmc/mouse_embryonic_stem_cell_culture.html

Category: Cell Biology and Cell Culture: ES Stem Cell Biology and Culture

Mouse Embryonic Stem Cell Culture. Southern Analysis of ES cells. The Darwin Transgenic Core Facility - [Read ES mouse cell stem cell Culture]

Fixation Protocol - http://cmif.osu.edu/419.cfm

Category: Histology Protocols: Fixation of Cells Tissues Protocol

There are many variations based on the type of tissue to be examined, whether one is dealing with cell suspensions, biopsies, perfused tissues, or monolayer . Fixation Protocol. OSU Campus Microscopy & Imaging Facility :: The Ohio State University College of Med - [Read Fixation Protocol]

Immunofluorescence Reagents and Protocols - http://www.molbio1.princeton.edu/facility/flowcyt/if1.html

Category: Molecular Imaging: Immunofluorescence Microscopy

Immunofluorescence Reagents and Protocols. Staining Protocols. Princeton. - [Read Immunofluorescence Reagents and Protocols]

Indirect Immunofluorescence Labeling Protocol - http://www.biotech.iastate.edu/facilities/CELLHYB/Indirect.htm

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols

An Indirect Immunofluorescence Labeling Protocol for flow cytometry. Iowa State University, Flow Cytometry Facility - [Read Indirect Immunofluorescence Labeling Protocol]

Negative Stain Electron Microscopy of Microtubules Protocol - http://mitchison.med.harvard.edu/protocols/EM.html

Category: Cytoskeleton Protocols: Microtubules Protocols

Negative staining is a rapid, qualitative method for analyzing microtubule structure at the EM level. Because negative staining involves deposition of heavy atom stains, structural artifacts such as flattening of the cylindrical microtubule and opening up of microtubules into flat sheets are common. Negative staining is very useful because of its ease, rapidity and lack of requirement for specialized equipment other than that found in a regular EM facility. - [Read Negative Stain Electron Microscopy of Microtubules Protocol]

Preparation of cell lysates from yeast using a French Press - http://www.embl.de/ExternalInfo/protein_unit/draft_frames/protocol_database/Purification/ec_yeast_press.html

Category: Protein Protocols: Protein Extraction From Cells

Preparation of cell lysates from yeast using a French Press. he Protein Expression and Purification Facility, EMBL - [Read Preparation of cell lysates from yeast using a French Press]

Preparation of cell lysates from yeast using glass beads vortexing - http://www.embl.de/ExternalInfo/protein_unit/draft_frames/protocol_database/Purification/ec_glass_beads.html

Category: Protein Protocols: Protein Extraction From Cells

Preparation of cell lysates from yeast using glass beads vortexing. Includes lysis buffer recipes. The Protein Expression and Purification Facility, EMBL - [Read Preparation of cell lysates from yeast using glass beads vortexing]

Protocol for Manual Peptide Synthesis - http://www.biotech.unl.edu/proteomics/protocol.html

Category: Peptide Protocols

Protocol for Manual Peptide Synthesis. Resin Swelling And Coupling Of Activated Amino Acid Esters. Deblocking and cleaving peptide from solid support. Synthesis of Peptides. The University of Nebraska-Lincoln Protein Core Facility - [Read Protocol for Manual Peptide Synthesis]

Protocols for Mass Spectrometry Analysis Preparation - http://www.st-andrews.ac.uk/~bmsmspf/protocols.htm#manual

Category: Mass Spectrometry Protocols

Protocols for Mass Spectrometry Analysis Preparation. Preparing gel slices for PMF or MS/MS , mass spec data interpretation, Submitting proteins in solution, Interpretation of LC-MS/MS data. BMS MASS SPECTROMETRY AND PROTEOMICS FACILITY - [Read Protocols for Mass Spectrometry Analysis Preparation]

Quantitative Flow Cytometry for Apoptosis - http://www.biotech.uiuc.edu/centers/Proteomics/Flow_cytometry/docs/Apopbroc.pdf

Category: Cell Biology and Cell Culture: Apoptosis Protocols

Quantitative Flow Cytometry for Apoptosis Detection. Flow Cytometry Facility, U.Illinois. - [Read Quantitative Flow Cytometry for Apoptosis]