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Extraction and Solubilization of Total Protein from Microorganisms Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4224

Category: Bacterial Cell Culture Protocols

The growth conditions of microbial cell cultures and the time of sample collection should be optimized and standardized when growing cells for protein extraction. Because cells may excrete proteases and other extracellular enzymes, and compounds in the medium may interfere with extraction, wash cultures with an isotonic buffer, such as PBS or sucrose before solubilization. - [Read Extraction and Solubilization of Total Protein from Microorganisms Protocol]

Extraction and Solubilization of Total Protein from Plant Seeds Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4225

Category: Plant Biology Protocols: Plant Proteins, Peptides and Antigens

Protocol on the extraction and solubilization of total protein from plant seeds. - [Read Extraction and Solubilization of Total Protein from Plant Seeds Protocol]

Extraction Method for Formalin Pigments from Tissue Sections Protocol - http://www.bio.com/protocolstools/protocol.jhtml?id=p1015

Category: Lipid Protocols

Protocol for extraction method for formalin pigments from tissue sections. - [Read Extraction Method for Formalin Pigments from Tissue Sections Protocol]

Extraction of Bacteriophage lambda DNA from Large-scale Cultures Using Formamide Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3973

Category: Viral Manipulation Protocols: Phage Lamda Manipulation Protocols

Formamide can be used instead of proteinase K to dissociate bacteriophage {lambda} coat proteins from the viral {lambda}. The procedure is rapid and works best with large-scale preparations of bacteriophage {lambda}. - [Read Extraction of Bacteriophage lambda DNA from Large-scale Cultures Using Formamide Protocol]

Extraction of Bacteriophage lambda DNA from Large-scale Cultures Using Proteinase K and SDS Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3972

Category: Viral Manipulation Protocols: Phage Lamda Manipulation Protocols

DNA is best isolated from bacteriophage lambda particles by digesting the viral coat proteins with a powerful protease such as proteinase K, followed by extraction with phenol:chloroform. This procedure is easily adapted for use with smaller-scale preparations of bacteriophage lambda. - [Read Extraction of Bacteriophage lambda DNA from Large-scale Cultures Using Proteinase K and SDS Protocol]

Extraction of Calf Brain Lipids Protocol - http://www.bio.com/protocolstools/protocol.jhtml?id=p1281

Category: Lipid Protocols

Protocol for the extraction of calf brain lipids. Protocol describes a rapid method to isolate lipids from bovine brain tissue using an organic solvent mixture of Chloroform and Methanol. - [Read Extraction of Calf Brain Lipids Protocol]

Extraction of Geminiviral DNA from a Highly Mucilaginous Plant Protocol - http://pubs.nrc-cnrc.gc.ca/ispmb/ispmb18/R00-056.pdf

Category: Viral Protocols

Protocol for the extraction of geminiviral DNA from a highly mucilaginous plant (Abelmoschus esculentus). Includes: Extraction; Alkali lysis; Cloning. - [Read Extraction of Geminiviral DNA from a Highly Mucilaginous Plant Protocol]

Extraction of the SecinH3 from Mouse Liver Protocol - http://www.natureprotocols.com/2006/12/15/extraction_of_the_secinh3_from.php

Category: Mouse Protocols

Mice fed with the cytohesin inhibitor SecinH3 for two days develop hepatic insulin resistance that can be identified by reduced liver glycogen levels, increased serum insulin and ketone body levels and decreased serum non-esterified fatty acid. To confirm the presence and identity of SecinH3 in mouse liver, we extracted the compound from liver homogenates with chloroform and identified it by LC/MS. - [Read Extraction of the SecinH3 from Mouse Liver Protocol]

Extraction of Total Protein from Arabidopsis Protocol - http://www.cshprotocols.org/cgi/content/abstract/2007/1/pdb.prot4680

Category: Plant Biology Protocols: Arabidopsis Protein Peptide Protocols

The simplest way to analyze proteins is in unfractionated extracts. However, it is often desirable to fractionate proteins, e.g, by size. This procedure extracts total protein from Arabidopsis samples. Typical yields are ~2-3 mg/ml (using rosette leaves) or 6-8 mg/ml (using young seedlings). - [Read Extraction of Total Protein from Arabidopsis Protocol]

Fungal DNA Isolation Method - http://wheat.pw.usda.gov/~lazo/methods/lazo/dnafun.html

Category: DNA Protocols: DNA Extraction Protocols

DNA isolation from various fungal species including: Cochliobolus, Aternaria, and Fusarium. Key steps: (1) the use of young lyophilized mycelial mats - yield less contaminating carbohydrates; (2) proteinase K in the extraction buffer to destroy DNases (f - [Read Fungal DNA Isolation Method]

Fungal DNA Isolation Protocol - http://wheat.pw.usda.gov/~lazo/methods/lazo/dnafun.html

Category: Nucleic Acid Purification Protocols: DNA Isolation Protocols

Protocol for fungal DNA isolation. The key elements in this prep are (1) the use of young lyophilized mycelial mats....young mats (4 days growth for C. carbonum)...yield less contaminating carbohydrates and other misc. junk (2) lots of proteinase K in the extraction buffer to kill Dnases (final =0.3mg/ml). - [Read Fungal DNA Isolation Protocol]

Genotyping Protocol: T1320 mouse - http://www.mmrrc.org/strains/425/ctr_protocol.pdf

Category: Molecular Model Organisms: Mouse Animal Protocols: Mouse Genotyping Protocols

Genotyping Protocol: MMRRC 425. Assay Type: PCR - can distinguish heterozygous animals from homozygous animals. DNA Extraction: DNA from tail snips was ... - [Read Genotyping Protocol: T1320 mouse]

Getting DNA from Old Dead Stuff - http://www.uga.edu/srel/DNA_Lab/OldDNA99.rtf

Category: DNA Protocols: DNA Extraction Protocols: Extracting Ancient DNA

DNA Isolation from museum specimens: 1) what's the best tissue to sample; 2) what's the best way to get DNA out of that tissue? 1) the easiest well-dried tissue to get, and 2) the easiest extraction methods you are comfortable doing. Travis Glenn. - [Read Getting DNA from Old Dead Stuff]

In-gel digestion of proteins to be analyzed by mass spectrometry - http://www.narrador.embl-heidelberg.de/GroupPages/PageLink/activities/protocols/ingeldigest.html

Category: Proteomic Protocols

In-Gel Digestion Protocol. Excision of protein bands (spots) from polyacrylamide gels Reduction and alkylation . Reduction and alkylation . MALDI analysis of the supernatant after in-gel digestion. Extraction of Peptides.Matthias Wilm EMBL Bioanalytical R - [Read In-gel digestion of proteins to be analyzed by mass spectrometry]

Lipid Extraction from Plasma Membrane Fractions Prepared from Yeast Cells Protocol - http://www.bio.com/protocolstools/protocol.jhtml?id=p419

Category: Lipid Protocols

Protocol for lipid extraction from plasma membrane fractions prepared from yeast cells. - [Read Lipid Extraction from Plasma Membrane Fractions Prepared from Yeast Cells Protocol]

Lipid Extraction from Yeast Cells Protocol - http://www.bio.com/protocolstools/protocol.jhtml?id=p420

Category: Lipid Protocols

Protocol for lipid extraction from yeast cells. - [Read Lipid Extraction from Yeast Cells Protocol]

Lysing Tissue-Culture Cells for Immunoprecipitation Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/23/pdb.prot4531

Category: Cell Culture Protocols: Cell Lysis Protocols

For cells grown in tissue culture, the most useful method of lysis is treating with detergents, as described in this protocol. Non-ionic detergents, such as NP-40, solubilize the plasma and intracellular membranes, break many weak intermolecular bonds, and solubilize most of the commonly studied protein antigens. RIPA lysis buffer may be used as a more rigorous extraction buffer to release all but the insoluble proteins of the cell and to break most weak noncovalent interactions. - [Read Lysing Tissue-Culture Cells for Immunoprecipitation Protocol]

Lysis and Protein Extraction from WEHI-231 Cells with TriPure Isolation Reagent Protocol - http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi/afcs_PP00000162.pdf?pid=PP00000162

Category: Chromatography Protocols: Immobilized Metal Ion Affinity Chromatography Protocols

Protocol is the first step in a three-step process for the preparation and enrichment of phosphopeptides using immobilized metal affinity chromatography (IMAC) for the identification of the phosphopeptides by LC-MS/MS. This procedure is used to prepare protein extracts from WEHI-231 cells. This preparation method provides total cellular protein samples that are free of contaminating nucleic acids. - [Read Lysis and Protein Extraction from WEHI-231 Cells with TriPure Isolation Reagent Protocol]

Method to Analyze Meiotic Segregation Patterns in Coprinus cinereus Using PCR - http://www.fgsc.net/fgn/freedman.html

Category: Fungi Protocols: Fungi Genetics Protocols

Quick and reliable method to analyze meiotic segregation patterns in Coprinus cinereus using the polymerase chain reaction. The advantages of this method include: 1. The tissue is grown and lyophilized in the same tube, which facilitates the simultaneous analysis of many segregants. 2. Only one extraction step is necessary. 3. The markers are scored by gel electrophoresis, thereby bypassing Southern analysis. - [Read Method to Analyze Meiotic Segregation Patterns in Coprinus cinereus Using PCR]

Methods for Plant RNA Isolation - http://wheat.pw.usda.gov/~lazo/methods/lazo/met6.html

Category: Plant Biology Protocols: Plant DNA RNA Isolation Protocols

AMES/Chloroform extraction to extract total RNA from potato leaves for viroid analysis. - [Read Methods for Plant RNA Isolation]

Microarrays a Complete Manual PDF - http://research.nhgri.nih.gov/microarray/Protocols.pdf

Category: DNA Microarray Protocols

Microarray fabrication, coating, slide blocking, printing microarrays, RNA extraction, Sample Labeling for analysis, hybridization, complete protocol. NHGRI - [Read Microarrays a Complete Manual PDF]

Mouse Tail DNA Extraction Protocol - http://www.ohsu.edu/nsi/faculty/reddyh/lab/protmsdn.html

Category: Mouse Protocols: Mouse Genetics Protocols

Protocol for mouse tail DNA extraction. - [Read Mouse Tail DNA Extraction Protocol]

Nuclear and Splicing Extract Preparation - http://penguin.uchc.edu/~intron/Protocols/NuclearS100%20Extract.html

Category: Protein Protocols: Nuclear Extraction Protocols

S100 extract cytosolic extraction preparation. Buffer A (Hypotonic Buffer), Low and High salt buffer recipes. Brent Graveley - [Read Nuclear and Splicing Extract Preparation]

One-Step Isolation of Plant DNA Suitable for PCR Amplification - http://pubs.nrc-cnrc.gc.ca/ispmb/ispmb19/r01-054.pdf

Category: Plant Biology Protocols: Plant DNA RNA Isolation Protocols

One step extraction for isolation of plant DNA. DNA suitable for amplification by PCR can be produced from leaf material smaller than 0.3 mm2 in less than 20 min & no tube changes. Method was tested on several plant species. Method was found to extract DNA that could be amplified without any further purification or treatment. The isolated DNA was amplified using a universal chloroplast primer set. The method was validated by comparing size of PCR products generated using standard DNA isolation. - [Read One-Step Isolation of Plant DNA Suitable for PCR Amplification]

Phage Protocols - DNA Gels - http://www.rpgroup.caltech.edu/~paul/protocols/phage/lambda/gel/

Category: Molecular Biology Protocols: Phage Protocols and Methods

Phenol/chloroform DNA extraction, running a gel for DNA from Phages. Rob Phillips Group: Physical Biology of the Cell - [Read Phage Protocols - DNA Gels]

Articles (1-3 of 3)

Populational Analysis of Genomic DNA Protocol

A protocol on the populational Analysis of genomic DNA.

Genomic DNA Labeling of Microarrays Protocol

DNA microarrays are an ordered arrangement of DNA molecules complementary to genes of interest that are "spotted" by robotic equipment onto a glass slide substrate. The expression of genes in cells can be monitored with microarrays by preparing cDNA from the mRNA of cells of interest and measuring the hybridization to the microarray. This protocol describes the labeling of genomic DNA for use as a probe for hybridization to the cDNA spotted on the array.

Acid Guanidinium Thiocyanate RNA Isolation Phenol Chloroform Extraction

A single step RNA isolation protocol using Phenol Chloroform Extraction and Acid Guanidinium Thiocyanate. This RNA isolation method uses the fact that guanidinium thiocyanate can simultaneously lyse the cells and inactive cellular RNAses during the initial RNA isolation step allow a single step in the method.

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