expansion Protocols

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Culture and Expansion of OT-1 TCR Transgenic T cells Protocol - http://www.natureprotocols.com/2007/01/29/culture_and_expansion_of_ot1_t.php

Category: Immunology: T Cell Protocols

Protocol for culture and expansion of OT-1 TCR transgenic T cells. - [Read Culture and Expansion of OT-1 TCR Transgenic T cells Protocol]

Expansion of Targeted ES Clones Protocol - http://escore.im.wustl.edu/htmldocs/xpandtes1.htm

Category: Stem Cell Protocols: Stem Cell Culture Protocols

Protocol for the expansion of targeted ES clones. - [Read Expansion of Targeted ES Clones Protocol]

Proliferative Assays for T Cell Function Protocols - https://catalog.invitrogen.com/index.cfm?fuseaction=iProtocol.unitSectionTree&treeNodeID=9E6634679E96E622003E15187F105306&objectid=6674B7F5E219FD4021F448C7D13AF27A

Category: Immunology: T Cell Protocols

Protocols describe the conditions required to induce proliferation are described. Also describe the assay in which CD4·CD25·T cells are co-cultured with conventional T cells in order to assess their suppressive function. Will describe the culture conditions for the activation and expansion of CD4·CD25· cells. - [Read Proliferative Assays for T Cell Function Protocols]

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Immobilized Antigen Phage Antibody Selection Protocol

A protocol for the selection of Phage Antibodies using Immobilized Antigen. This method describes the selection of antibodies from bacteriophage antibody libraries that recognize a specific antigen. The phage display library of antibody-displaying phage particles is exposed to antigen attached to a solid substrate (Nunc Immuno™ tubes). The phage particles with affinity for antigen bind to the immobilized antigen and are selected from the library of phage expressing antibodies.

Picking Transfected ES Cell Clones Protocol

This protocol a protocol on how to generate transfected embryonic stem (ES) cell clones. The previous protocol in this series is the Protocol for Electroporation of ES cells. The next protocol in the series is the Protocol on Disaggregation, Expansion, and Freezing of Transfected ES Clones.

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