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Column Chromatography Protocol - http://www.biochem.arizona.edu/classes/bioc463a/Info/protocols/exp_4_1.pdf

Category: Chromatography Protocols: Column Chromatography Protocols

Size Exclusion Column Chromatography Protocol. PDF. In this protocol you will learn how to use three types of column chromatography: Gel Filtration or Size Exclusion (SEC), Ion Exchange (IEC), and affinity (AC) in order to purify proteins and enzymes based on the physical properties of these biomolecules. Univ. Arizona, Biochemistry. - [Read Column Chromatography Protocol]

Dust Toxicity in Rat Alveolar Macrophage Cultures Protocol - http://embryo.ib.amwaw.edu.pl/invittox/prot/32.htm

Category: Pharmacology and Toxicology Protocols: Cytotoxicity Protocols: Cytotoxicity Assays in Cell Culture Protocols

Macrophage cells in culture may be exposed to particulate matter, and resultant effects upon cell viability determined by vital dye exclusion and enzyme leakage assays. - [Read Dust Toxicity in Rat Alveolar Macrophage Cultures Protocol]

Human Skin Fibroblast/Collagen Lattice Cytotoxicity Test - http://embryo.ib.amwaw.edu.pl/invittox/prot/27.htm#TOP

Category: Pharmacology and Toxicology Protocols: Cytotoxicity Protocols: Cell Specific Cytotoxicity Assays Protocols

Skin fibroblasts are incorporated into 3-D collagen lattices containing the test compounds. An inhibition of lattice contraction indicates a possible toxic effect which is verified by trypan blue exclusion for cell viability. - [Read Human Skin Fibroblast/Collagen Lattice Cytotoxicity Test]

Use of PCR to Prepare a Double-Stranded DNA Library Encoding Random Peptides Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/14/pdb.prot4135

Category: DNA Protocols: cDNA Library Protocols: cDNA Library Construction Protocols

PCR is used as a preparative tool for the synthesis of a high-complexity double-stranded DNA library. In the example presented here, a mixture of synthetic oligonucleotides is used to synthesize a random peptide NNK library, where K is either T or G. The exclusion of A and C nucleotides at the third position decreases the occurrence of stop codons but still allows codons for all 20 amino acids. - [Read Use of PCR to Prepare a Double-Stranded DNA Library Encoding Random Peptides Protocol]