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Attaching Adaptors to Protruding Termini Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3920

Adaptors are short double-stranded synthetic oligonucleotides that carry an internal restriction endonuclease recognition site and single-stranded tails at one or both ends. Adaptors are used to exchange restriction sites at the termini of linear DNA molecules. They may be purchased in phosphorylated and unphosphorylated forms. - [Read Attaching Adaptors to Protruding Termini Protocol]

Column Chromatography Protocol - http://www.biochem.arizona.edu/classes/bioc463a/Info/protocols/exp_4_1.pdf

Category: Chromatography Protocols: Column Chromatography Protocols

Size Exclusion Column Chromatography Protocol. PDF. In this protocol you will learn how to use three types of column chromatography: Gel Filtration or Size Exclusion (SEC), Ion Exchange (IEC), and affinity (AC) in order to purify proteins and enzymes based on the physical properties of these biomolecules. Univ. Arizona, Biochemistry. - [Read Column Chromatography Protocol]

Generation of Gene Deletions and Gene Replacements in Escherichia coli Protocol - http://www.biologicalprocedures.com/bpo/arts/1/123/m123.pdf

Category: E Coli Protocols: E coli Genetics Protocols

Protocol for the generation of gene deletions and gene replacements in Escherichia coli O157:H7 using a temperature sensitive allelic exchange system. Technology requires flanking DNA to be cloned into a temperature sensitive vector but the resulting clone allows great flexibility for further modification of the target sequence. It is therefore highly suited to the study of genes in which several rounds of changes are envisaged. - [Read Generation of Gene Deletions and Gene Replacements in Escherichia coli Protocol]

Protein Purification: Column Chromatography - Ion exchange; Dialysis and Concentration - http://wine1.sb.fsu.edu/bch5425/lect30/lect30.htm

Category: Protein Protocols: Protein Purification and Identification

Protein Purification: Column Chromatography - Ion exchange; Dialysis and Concentration. Blaber lecture - [Read Protein Purification: Column Chromatography - Ion exchange; Dialysis and Concentration]

T4 Polynucleotide Kinase Protocol Promega - http://www.promega.com/tbs/tb519/tb519.pdf

Category: DNA Protocols: T4 Polynucleotide Kinase Protocols

T4 Polynucleotide Kinase Protocol Promega. 5Â´ End-Labeling Protocol, Non-Radioactive Kinase Reaction, Exchange Reaction Protocol Using Protruding 5Â´ Phosphoryl Termini as Templates. Promega. T4 PNK Protocols. - [Read T4 Polynucleotide Kinase Protocol Promega]

Articles (1-2 of 2)

DNA Ligation Protocol

The DNA Ligation protocol described here contains the steps required to join together using ligase enzyme both plasmid DNA and insert DNA fragments in order to create a new plasmid. This new ligated plasmid can be transformed after into competent bacteria to produce DNA for mini, midi or maxi-prep isolation.

Vector Design Protocol for Transgenic Mice Generation

The protocol gives general considerations for the design of targeting vectors for transgenic mice. The protocol shares tips in the design of knock-out and knock-in vectors and some of their strategies for producing homologously recombined embryonic stem cells.

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