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Absorbance Assay 205 nm - http://www.ruf.rice.edu/~bioslabs/methods/protein/abs205.html

Category: Protein Protocols: Protein Quantitation Concentration Protocols

Absorbance assay at 280 nm. This method is just as convenient as for absorbance at 280 nm. It may be preferred if there is excessive contamination by nucleic acids, since nucleic acids absorb very little radiation at 205 nm. Setting the wavelength is a bit tricky since 205 nm is right on the shoulder of the protein peak. - [Read Absorbance Assay 205 nm]

Fragmentation of DNA by Sonication Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/23/pdb.prot4538

Category: Cloning Protocols

Protocol describes a method for DNA fragmentation by sonication. During sonication, DNA samples are subjected to hydrodynamic shearing by exposure to brief periods of sonication. DNA that has been sonicated for excessive periods of time is extremely difficult to clone. - [Read Fragmentation of DNA by Sonication Protocol]