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Establishment of Stable Transfectant of CHO Lec Cells Protocol - http://www.cbrinstitute.org/labs/springer/protocols/jun_CHOlec.html

Category: Transfection Protocols: Stable Transfection Protocols

CHO Lec3.2.8.1 cells have 4 independent mutations in the N and O glycosylation pathways. When cultured with alpha-glucosidase I inhibitor N-butyl-deoxynojirimycin, glycoproteins produced in CHO Lec3.2.8.1 cells are completely susceptible to Endo H digestion. Endo H cleaves chitobiose, leaving a single N-linked N-acetylglucosamine per site which is ideal for maintenance of protein solubility and special carb-protein interactions, such as between the first N-acetyl glucosamine residue and tryp. - [Read Establishment of Stable Transfectant of CHO Lec Cells Protocol]

Fixing Attached Cells in Paraformaldehyde Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/21/pdb.prot4294

Category: Cell Culture Protocols: Cell Fixing Protocols

Treating cells with paraformaldehyde leads to the establishment of chemical cross-links between free amino groups. When the cross-links join different molecules, a latticework of interactions occurs that holds the overall architecture of the cell together. Commercial formaldehyde solutions are not recommended, because they lack the advantages of using a variable-length polymer, and the cells will simultaneously be fixed with the alcohol (usually methanol). - [Read Fixing Attached Cells in Paraformaldehyde Protocol]

Fixing Suspension Cells with Paraformaldehyde Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/21/pdb.prot4295

Category: Cell Culture Protocols: Cell Fixing Protocols

Method for Establishment and Maintenance of Leaf and Root Cell Cultures Arabidopsis thaliana - http://pubs.nrc-cnrc.gc.ca/ispmb/ispmb19/r01-030.pdf

Category: Plant Biology Protocols: Arabidopsis Tissue Culture

Method for Establishment and Maintenance of Leaf and Root Cell Cultures Arabidopsis thaliana. CL ENCINA1, M CONSTANTIN2 and J BOTELLA2. Plant Molecular Biology Reporter 19: 245â€“248, 2001. - [Read Method for Establishment and Maintenance of Leaf and Root Cell Cultures Arabidopsis thaliana]

PCR Amplification of Highly GC-Rich Regions Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4093

Category: PCR Protocols: PCR Optimization

Procedure details the establishment of an amplification procedure for GC-rich sequences. The DNA fragments of interest are amplified in the presence of either 5% DMSO, 1 M betaine, 2 M betaine, 1 M betaine, and 5% DMSO; 2 M betaine and 5% DMSO; 0.4 M tetramethylene sulfone; or without any of the enhancers. - [Read PCR Amplification of Highly GC-Rich Regions Protocol]