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Bradford Protein Assay - http://www.ruf.rice.edu/~bioslabs/methods/protein/bradford.html

Category: Protein Protocols: Protein Quantitation Concentration Protocols: Bradford Protein Assay Protocols

A detailed and well thought out Bradford protein assay page using a spectrophotometer. Includes information such as the Bradford assay is very fast and uses about the same amount of protein as the Lowry assay, comments, procedural steps, equipment used and more. - [Read Bradford Protein Assay]

Column Chromatography Protocol DOC - http://tesla.ccrc.uga.edu/group_pages/protocols/downloads/columnprot.doc

Category: Chromatography Protocols: Column Chromatography Protocols

Column Chromatography Protocol DOC, includes troubleshooting information. Equipment used included: Peristaltic pump, UV detector , Chart recorder, and Column. Prestegard Lab. - [Read Column Chromatography Protocol DOC]

Complete Microarray Chip Protocol - http://www.rbhrfcrc.qimr.edu.au/kidney/Pages/Microarray%20protocol.html

Category: DNA Microarray Protocols

Reagents & Equipment, Labelling, Purification, Hybridisation, Washing. KRL Kidney Research Dr.Healt - [Read Complete Microarray Chip Protocol]

Detailed 2D Gel Electrophoresis - http://www.purdue.edu/dp/bbc_labs/2d.html

Category: Proteomic Protocols: 2-D Gel Electrophoresis

Excellent Detailed Protocol for 2-D Gel Electrophoresis including staining, gel running, and equipment. Purdue PSAL. - [Read Detailed 2D Gel Electrophoresis]

DNA Ligation Protocol PDF - http://www.oup.co.uk/pdf/pas/9v1-7-9.pdf

Category: Molecular Biology Protocols: DNA Ligation Protocols

DNA ligation Method, Equipment and reagents, T4 DNA ligase. Gannon and Powell. Oxford Practical Approach Series. - [Read DNA Ligation Protocol PDF]

Laboratory Survival Manual - http://keats.admin.virginia.edu/lsm/home.html

Category: General Research Protocols and Methods

Laboratory Survival Manual. Lab Safety, First AID, Chemicals and Equipment. Vaughn C. Kowahl - [Read Laboratory Survival Manual]

List of Protein Assays - http://www.ruf.rice.edu/~bioslabs/methods/protein/protein.html

Category: Protein Protocols: Protein Quantitation Concentration Protocols

Find a list of assays for the determination of protein concentration in a solution. This list includes the sensitivity range, volume/amount of sample needed, subjective comments on accuracy and convenience, and major interfering agents. Procedural details, equipment requirements, and references are outlined in the individual assay documents. - [Read List of Protein Assays]

Microinjection of Protein Samples Protocol - http://www.cshprotocols.org/cgi/content/abstract/2007/1/pdb.prot4657

Category: Microinjection Protocols: Microinjection of Proteins Protocols

This protocol describes a method for microinjecting proteins into the nucleus or cytoplasm of adherent cells. Microinjection equipment can be obtained from a number of suppliers; this protocol has been used with the Narishige IM-200 air pressure regulator and the Leitz micromanipulator. Using this system, it is possible to microinject a constant volume within a 50% difference among cells. - [Read Microinjection of Protein Samples Protocol]

Multiphoton Images from LSM 510 NLO System - http://www.udel.edu/bio/research/facilities/microscopy/equipment/multimgs.html

Category: Microscopy Protocols: Fluorescence Microscopy Protocols

Multiphoton fluorescence microscopy is a powerful new technology that enables the acquisition of optical sections without the use of a pinhole aperture typically used for confocal microscopy. The technique is based upon the two-photon principle: A fluorescent molecule simultaneously absorbs two photons producing an electronic transition from the ground to excited state equal to two times the energy of each incident photon. - [Read Multiphoton Images from LSM 510 NLO System]

Negative Stain Electron Microscopy of Microtubules Protocol - http://mitchison.med.harvard.edu/protocols/EM.html

Category: Cytoskeleton Protocols: Microtubules Protocols

Negative staining is a rapid, qualitative method for analyzing microtubule structure at the EM level. Because negative staining involves deposition of heavy atom stains, structural artifacts such as flattening of the cylindrical microtubule and opening up of microtubules into flat sheets are common. Negative staining is very useful because of its ease, rapidity and lack of requirement for specialized equipment other than that found in a regular EM facility. - [Read Negative Stain Electron Microscopy of Microtubules Protocol]

Optimized Protocol for Mounting Tissue Sections PDF - http://arctur.com/research_portal/images/Application%20Notes/pen_membrane_protocol_9.pdf

Category: Histology Protocols: Tissue Sample Sectioning

Generally Required Materials and Equipment for Tissue Sectioning. Sectioning and Slide Preparation Procedure onto Metal-Framed PEN Membrane Slides. Arcturus. - [Read Optimized Protocol for Mounting Tissue Sections PDF]

Principles of Aseptic Technique: ESSENTIALS FOR ANIMAL RESEARCH - http://research.ucsb.edu/connect/acc/esasept.htm

Category: Animal Research Techniques

Facilities, equipment, sterilization, steam, gas, liquid, animal research, preparation of the animal, surgical pack, surgeon preparation. - [Read Principles of Aseptic Technique: ESSENTIALS FOR ANIMAL RESEARCH]

Radiation Safety Manual - http://www.cdc.gov/OD/ohs/manual/radman.htm

Category: Radioactivity: Radioaction Safety

Administration of the Radiation Safety Program, , Physical Security of Radioactive Materials, "As Low as Reasonably Achievable" (ALARA) Philosophy, Authorized Users, Radiation Workers , Permitted Workers, Training, Procurement, Inventory and Records, Personnel Monitoring, Radiation Exposure During Pregnancy, Laboratory Supplies and Equipment, Bioassay, Labeling, Surveys, Radioactive Waste Disposal. U.S. Department of Health and Human Services, CDC, Atlanta, GA. - [Read Radiation Safety Manual]

Selection of Appropriate Imaging Equipment and Methodology for Live Cell Imaging in Drosophila - http://www.cshprotocols.org/cgi/content/abstract/2006/29/pdb.ip21

Category: Microscopy Protocols: In Vivo Imaging Protocols

In the past two decades, there have been many revolutions in light microscopy techniques made possible by improvements in optics, detector technology, and computers. Furthermore, there is no indication that the rate of development of new equipment is slowing down. Here we attempt to provide an overview of available options and important considerations applicable to imaging Drosophila cells and tissues. - [Read Selection of Appropriate Imaging Equipment and Methodology for Live Cell Imaging in Drosophila]

Selection of Appropriate Imaging Equipment and Methodology for Live Cell Imaging in Drosophila - http://www.cshprotocols.org/cgi/content/abstract/2006/29/pdb.ip21

Category: Pharmacology and Toxicology Protocols: Drug Discovery

Protocol for selection of appropriate imaging equipment and methodology for live cell imaging in Drosophila. - [Read Selection of Appropriate Imaging Equipment and Methodology for Live Cell Imaging in Drosophila]

Studying Mitosis in Cultured Mammalian Cells Protocol - http://www.cshprotocols.org/cgi/content/abstract/2007/3/pdb.prot4674

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Cell Cycle Analysis Protocols

Protocol describes methods for maintaining healthy, dividing mammalian cells in culture and during imaging, when mitosis can be examined. Rose chambers are preferable for observation and microinjection of living mitotic cells, but slide/coverslip preparations are easy to make and do not require any special equipment. - [Read Studying Mitosis in Cultured Mammalian Cells Protocol]

Studying Mitosis in Cultured Mammalian Cells Protocol - http://www.cshprotocols.org/cgi/content/abstract/2007/3/pdb.prot4674

Category: Cytogenetics Protocols

Protocol describes methods for maintaining healthy, dividing mammalian cells in culture and during imaging, when mitosis can be examined. Rose chambers are preferable for observation and microinjection of living mitotic cells, but slide/coverslip preparations are easy to make and do not require any special equipment. Another inexpensive and easy-to-use alternative is to grow cells in a culture dish with a glass bottom. Such dishes are suitable for microinjection experiments. - [Read Studying Mitosis in Cultured Mammalian Cells Protocol]

Studying Mitosis in Cultured Mammalian Cells Prtocol - http://www.cshprotocols.org/cgi/content/abstract/2007/3/pdb.prot4674

Category: Cell Biology and Cell Culture: Cell Cycle Protocols: Mitosis Protocols

Protocol describes methods for maintaining healthy, dividing mammalian cells in culture and during imaging, when mitosis can be examined. Rose chambers are preferable for observation and microinjection of living mitotic cells, but slide/coverslip preparations are easy to make and do not require any special equipment. Another inexpensive and easy-to-use alternative is to grow cells in a culture dish with a glass bottom. Such dishes are suitable for microinjection experiments. - [Read Studying Mitosis in Cultured Mammalian Cells Prtocol]

Time-Lapse Cinematography in Living Drosophila Tissues: Preparation of Material - http://www.cshprotocols.org/cgi/content/abstract/2006/29/pdb.prot4602

Category: Microscopy Protocols: In Vivo Imaging Protocols

Sophisticated fluorescence microscopy methods & equipment, now allow cellular events to be studied at high resolution in living material. The studying of living fly tissues presents unique difficulties in keeping the cells alive, introducing fluorescent probes, & imaging through thick hazy cytoplasm. This protocol outlines the preparation of major tissue types amenable to study by time-lapse cinematography and different methods for keeping them alive. - [Read Time-Lapse Cinematography in Living Drosophila Tissues: Preparation of Material]

Widefield Microscopy Nikon Upright with Operating Instructions - http://depts.washington.edu/keck/Widefield/equipment.htm

Category: Molecular Imaging

Widefield Microscopy Nikon Upright with Operating Instructions. Keck Imaging Center. - [Read Widefield Microscopy Nikon Upright with Operating Instructions]

Articles (1-4 of 4)

Genomic DNA Labeling of Microarrays Protocol

DNA microarrays are an ordered arrangement of DNA molecules complementary to genes of interest that are "spotted" by robotic equipment onto a glass slide substrate. The expression of genes in cells can be monitored with microarrays by preparing cDNA from the mRNA of cells of interest and measuring the hybridization to the microarray. This protocol describes the labeling of genomic DNA for use as a probe for hybridization to the cDNA spotted on the array.

Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA

This Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA protocol describes the production of probes labeled with the fluorescent dyes, Cy3 and Cy5, following the synthesis of cDNA from human mRNA and the hybridization of the probes to DNA microarrays.

3' Rapid Amplification of cDNA Ends RACE Using PCR Protocol

3' Rapid Amplification of cDNA Ends RACE Using PCR Protocol. This protocol contains the steps for 3' end rapid amplification of mRNA by PCR. The first-strand cDNA is synthesized from total or poly(A+) RNA by priming from the poly-A tail of the mRNA using a oligo (dT) adaptor primer. The cDNA is then amplified via PCR using a gene-specific primer and an adaptor primer.

Electrotransformation of BMH 81-17mut S for Isolating Site-Directed dsDNA Mutants

This protocol describes the electroporation of the BMH 81-17 mut S strain that is recommended for tranformation of the site directed mutagenesis of dsDNA (See Protocol on Site-Directed Mutagenesis on Double Stranded DNA). BMH 81-17 mut S are a mismatch repair defective (mut S) Escherichia coli strain. The probability that the two mutations will cosegregate during the first round of DNA replication is increased in this strain.

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