epithelial Protocols

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IMEC Maintenance - http://www.flemingtonlab.com/Protocols/IMECMaintCulturingMisc.pdf

Category: Primary Cell Isolation and Culture Protocols: Mammalian Cell Culture Protocols

Information on Immortalized Mammary Epithelial Cells (IMEC). Includes: Background Information on IMEC; Culturing; Trypsinization. - [Read IMEC Maintenance]

Isolation of Primary Epithelial Cells - http://www.lbl.gov/lifesciences/BissellLab/labprotocols/primarycellprep.htm

Category: Cell Biology and Cell Culture: Epithelial Cell Culture

Isolation of Primary Epithelial Cells. Digestion: In Collagenase Medium, Purification and epithelial cell culture methods. Bissell Lab. - [Read Isolation of Primary Epithelial Cells]

Lung Cell Assay Protocol - http://embryo.ib.amwaw.edu.pl/invittox/prot/48.htm

Category: Pharmacology and Toxicology Protocols: Cytotoxicity Protocols: Cell Specific Cytotoxicity Assays Protocols

Potential embryotoxicity is assessed by monitoring the effect of the test compound on total protein synthesis, and DNA synthesis in cultured human foetal lung fibroblasts. Rat lung epithelial cells can be used to determine cytotoxicity of select compounds because of their ability to metabolise xenobiotics. - [Read Lung Cell Assay Protocol]

Protocol for the Isolation of Pancreatic Duct Epithelial Cells - http://pen2.igc.gulbenkian.pt/cftr/vr/b/gray_protocol_for_the_isolation_of_pancreatic_duct.pdf

Category: Cell Biology and Cell Culture: Epithelial Cell Culture

Protocol for the Isolation of Pancreatic Duct Epithelial Cells. Gray and Argent. - [Read Protocol for the Isolation of Pancreatic Duct Epithelial Cells]

Transient Transfection Into 293T Cells Protocol - http://www.cbrinstitute.org/labs/springer/protocols/jun_293T.html

Category: Transfection Protocols: Transient Transfection Protocols

Transient transfection into 293T cells is a convenient way to overexpress and obtain both cellular and extracellular (secreted or membrane) proteins. 293 is a human renal epithelial cell line which is transformed by adenovirus E1A gene product. 293T is a derivative which also express SV40 large T antigen, allowing episomal replication of plasmids containing the SV40 origin and early promoter region. They (both) have the unusual property of being highly transfectable. - [Read Transient Transfection Into 293T Cells Protocol]

Worthington Tissue Dissociation Guide - http://www.tissuedissociation.com/techniques.html

Category: Cell Culture Protocols

Includes information on: Cell Isolation Theory ( Tissue Types: Epithelial Tissue, Connective Tissue); Dissociating Enzymes (Collagenase, Trypsin, Elastase, Hyaluronidase, Papain, Chymotrypsin, Deoxyribonuclease I, Neutral Protease (Dispase), Trypsin Inhibitor (Soybean)); Cell Isolation Techniques: Working With Enzymes, Basic Primary Cell Isolation, Equilibration with 95%O2:5%CO2, Trituration, Enzymatic Cell Harvesting, Cell Adhesion and Harvesting, Trypsin for Cell Harvesting, etc... - [Read Worthington Tissue Dissociation Guide]