enhancing Protocols

Search results for: enhancing

Protocols » Search Results

Search Results Protocol Links Sort by: Hits | Alphabetical

Enhancing PCR of Very Difficult Regions Protocol - http://www.genome.ou.edu/protocol_enhancing_PCR.html

Category: PCR Protocols: PCR Optimization

Protocol for enhancing PCR of very difficult regions. - [Read Enhancing PCR of Very Difficult Regions Protocol]

PEI Nanoparticles for Targeted Gene Delivery Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/1/pdb.prot4451

Category: Gene Delivery Protocols: DNA Nanoparticles Protocols

This protocol describes the preparation of polyethylenimine (PEI)/DNA nanoparticles for targeted gene delivery. This delivery strategy improves the efficiency of gene transfer by enhancing the entry of gene vectors into the desired cells and reducing uptake by nontarget cells. We describe here methods for the conjugation of targeting peptides to PEIs, formation of DNA complexes using the conjugated PEIs or nonconjugated PEIs together with targeting peptides, and cell transfection. - [Read PEI Nanoparticles for Targeted Gene Delivery Protocol]