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Establishment of Stable Transfectant of CHO Lec Cells Protocol - http://www.cbrinstitute.org/labs/springer/protocols/jun_CHOlec.html

Category: Transfection Protocols: Stable Transfection Protocols

CHO Lec3.2.8.1 cells have 4 independent mutations in the N and O glycosylation pathways. When cultured with alpha-glucosidase I inhibitor N-butyl-deoxynojirimycin, glycoproteins produced in CHO Lec3.2.8.1 cells are completely susceptible to Endo H digestion. Endo H cleaves chitobiose, leaving a single N-linked N-acetylglucosamine per site which is ideal for maintenance of protein solubility and special carb-protein interactions, such as between the first N-acetyl glucosamine residue and tryp. - [Read Establishment of Stable Transfectant of CHO Lec Cells Protocol]

Microarray Protocols - http://www.erasmusmc.nl/interne_oncologie/res/endo/Protocol%20for%20RNA%20amplification%2C%20labelling%20and%20hybridization%20to%20%20microarray.pdf

Category: Microarray Protocols: Microarray Analysis Protocols

Protocols for microarrays. Includes: RNA amplification by in vitro transcription; cDNA labeling; Hybridization of cDNA microarrays. - [Read Microarray Protocols]

Protocol for RNA Amplification by In Vitro Transcription - http://www.erasmusmc.nl/interne_oncologie/res/endo/Protocol%20for%20RNA%20amplification%2C%20labelling%20and%20hybridization%20to%20%20microarray.pdf

Category: Cell Biology and Cell Culture: In Vitro Reconstitution Protocols

RNA amplification by in vitro transcription protocol. Includes protocols on cDNA labeling and hybridization on cDNA microarrays. - [Read Protocol for RNA Amplification by In Vitro Transcription]

Articles (1-1 of 1)

Picking Transfected ES Cell Clones Protocol

This protocol a protocol on how to generate transfected embryonic stem (ES) cell clones. The previous protocol in this series is the Protocol for Electroporation of ES cells. The next protocol in the series is the Protocol on Disaggregation, Expansion, and Freezing of Transfected ES Clones.