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Transcript In Situ Hybridization of Whole-Mount Embryos for Phenotype Analysis of RNAi-Treated - http://www.cshprotocols.org/cgi/content/full/2006/21/pdb.prot4519

Category: Cytogenetics Protocols: In Situ Hybridization Protocols

This protocol fixes and prepares embryos for in situ hybridization to visualize transcript expression patterns. It is a modification of the method developed by Tautz and Pfeifle for whole-mount in situ analysis of embryos. Use of the standard hybridization protocol on RNAi-treated embryos results in high background staining, which makes visualization of transcript expression patterns practically impossible. The following modifications eliminate this problem and allow visualization of transcript. - [Read Transcript In Situ Hybridization of Whole-Mount Embryos for Phenotype Analysis of RNAi-Treated]

TUNEL in Bovine Embryos - http://www.animal.ufl.edu/hansen/protocols/tunel.htm

Category: Cell Biology and Cell Culture: Apoptosis Protocols: TUNEL assay apoptosis

TUNEL procedure protocol in Bovine Embryos. Paula-Lopes et al. - [Read TUNEL in Bovine Embryos]

TUNEL Staining Protocol for Xenopus - http://tropicalis.berkeley.edu/home/gene_expression/TUNEL/TUNEL.html

Category: Xenopus Protocols: Xenopus Embryo Manipulation Protocols

Protocol is effective in identifying apoptotic cells in embryos that are undergoing substantial apoptosis. - [Read TUNEL Staining Protocol for Xenopus]

Uterine Transfer Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/18/pdb.prot4380

Category: Mouse Protocols: Mouse Reproductive System Surgery Protocols

Protocol describes a procedure for uterine transfer, which is used for chimera production. The method is based on extensive work which resulted in the first successful development and birth of in-vitro-cultured mouse embryos. It is best to practice this procedure first on a cadaver and then on an anesthetized 2.5-days post coitum (dpc) pseudopregnant mouse using blue Affigel beads rather than embryos. - [Read Uterine Transfer Protocol]

Whole Mount Fluorescent Antibody Staining of Drosophila Embryos Protocol - http://vosshall.rockefeller.edu/protocols/EmbryoImmunofluo.pdf

Category: Drosophila Protocols: Drosophila Staining Protocols

Protocol for whole mount fluorescent antibody staining of Drosophila embryos. - [Read Whole Mount Fluorescent Antibody Staining of Drosophila Embryos Protocol]

Whole Mount In Situ Hybridization for the Detection of mRNA in Drosophila Embryos Protocol - http://www.roche-applied-science.com/PROD_INF/MANUALS/InSitu/pdf/ISH_208-215.pdf

Category: Drosophila Protocols: Drosophila Genetics Protocols

Protocol for whole mount in situ hybridization for the detection of mRNA in Drosophila embryos. Includes: Embryo collection and fixation; Prehybridization treatment; Hybridization; Washing and detection. - [Read Whole Mount In Situ Hybridization for the Detection of mRNA in Drosophila Embryos Protocol]

Whole Mount In Situ Hybridization of Mouse Protocol - http://www.hhmi.ucla.edu/derobertis/protocol_page/in_situ.html

Category: Mouse Protocols: Mouse Embryology Protocols

Protocol for whole mount in situ hybridization of mouse. Incluses: Preparation of the probe; General Comments; Preparation of embryos; In Situ hybridization. - [Read Whole Mount In Situ Hybridization of Mouse Protocol]

Whole-Mount In Situ Hybridization for the Detection of RNA in C. elegans Embryos Protocol - http://cobweb.dartmouth.edu/~ambros/worms/14.html

Category: C. elegans Protocols

Protocol for whole-mount in situ hybridization for the detection of RNA in C. elegans embryos. Includes: Collection of Embryos; Permeabilization and fixation of embryos; Prehybridization; Hybridization; Post-hybridization washes; Probe Detection; Double-labeling; Interpretation. - [Read Whole-Mount In Situ Hybridization for the Detection of RNA in C. elegans Embryos Protocol]

Whole-Mount Preparations for Analysis of Axonal Pathfinding in dsRNA-Treated Avian Embryos - http://www.cshprotocols.org/cgi/content/abstract/2006/23/pdb.prot4506

Category: RNAi Technology Protocols: RNAi Avian Embryos Protocols

Protocol describes a method for staining nerve fibers in whole-mount preparations of avian embryos using an antibody against the 160-kD subunit of neurofilaments. This allows the comparison of the branching pattern of motor and sensory neurons between control and experimental embryos. This protocol has been successfully applied for embryos at different stages up to about stage 33 (7 days of incubation). - [Read Whole-Mount Preparations for Analysis of Axonal Pathfinding in dsRNA-Treated Avian Embryos]

Whole-Mount Preparations for Analysis of Axonal Pathfinding in dsRNA-Treated Avian Embryos Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/23/pdb.prot4506

Category: Avian Bird Protocols

X-gal Staining of Wholemount Drosophila Embryos Protocol - http://jfly.iam.u-tokyo.ac.jp/html/manuals/pdf/E_Staining_Uemura.pdf

Category: Drosophila Protocols: Drosophila Staining Protocols

Protocol for X-gal staining of wholemount Drosophila embryos. - [Read X-gal Staining of Wholemount Drosophila Embryos Protocol]

Articles (1-1 of 1)

Acid Guanidinium Thiocyanate RNA Isolation Phenol Chloroform Extraction

A single step RNA isolation protocol using Phenol Chloroform Extraction and Acid Guanidinium Thiocyanate. This RNA isolation method uses the fact that guanidinium thiocyanate can simultaneously lyse the cells and inactive cellular RNAses during the initial RNA isolation step allow a single step in the method.

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