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Isolation and Freezing of Primary Mouse Embryonic Fibroblasts (MEF) For Feeder Plates - http://www.cshprotocols.org/cgi/content/extract/2006/18/pdb.prot4482

Category: Stem Cell Protocols: Feeder Cell Preparation Protocols

This protocol describes the isolation of fibroblasts from mouse embryos. Mouse embryonic fibroblast (MEF) cells are used as a feeder layer for the culture of mouse embryonic stem (ES) cells to help maintain them as pluripotent stem cells. The inhibition of ES-cell differentiation provided by the MEF feeders appears to be due to their production of leukemia inhibitory factor (LIF). - [Read Isolation and Freezing of Primary Mouse Embryonic Fibroblasts (MEF) For Feeder Plates]

Fixation of Mouse Embryos and Tissues Protocol - http://www.cshprotocols.org/cgi/content/abstract/2007/1/pdb.prot4702

Category: Mouse Protocols: Mouse Embryology Protocols

Protocol provides methods for fixation of mouse embryos and tissues with paraformaldehyde, Bouin’s fixative, or methanol/DMSO solution. - [Read Fixation of Mouse Embryos and Tissues Protocol]

Combined LacZ staining and in situ hybridization PDF - http://www.oup.co.uk/pdf/pas/13-3-9.pdf

Category: Transgenic Mouse Protocols: Mouse Genotyping Protocols

Practical Approach Online, Oxford University Press. Combined LacZ staining and in situ hybridization of mouse embryos, beta-galactosidase. - [Read Combined LacZ staining and in situ hybridization PDF]

Aggregation of ES cells and eight cell stage embryos - http://web.archive.org/web/20021020163836/grimwade.biochem.unimelb.edu.au/bowtell/cellbiol/sect65.htm#Aggregation%20of%20ES%20cells%20and%20eight%20cell%20stage%20embryos

Category: Cell Biology and Cell Culture: ES Stem Cell Biology and Culture

Aggregation of ES cells and eight cell stage embryos. Bowtell Lab. - [Read Aggregation of ES cells and eight cell stage embryos]

Isolation of Fibroblasts from Mammalian Embryos Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/18/pdb.prot4474

Category: Primary Cell Isolation and Culture Protocols: Mammalian Cell Culture Protocols

This protocol describes the isolation of fibroblasts from mouse, rat, and hamster embryos. - [Read Isolation of Fibroblasts from Mammalian Embryos Protocol]

Mouse Gonad Removal and Germ Cell Staining Protocol - http://www.swarthmore.edu/NatSci/sgilber1/DB_lab/Mouse/mouse_gonads.html

Category: Mouse Protocols: Mouse Reproductive System Surgery Protocols

Protocol describes how to remove the gonads, determine the sex ofmouse embryos 13.5 days post coitum and visualize the germ cells, through use of an alkaline phosphatase staining. - [Read Mouse Gonad Removal and Germ Cell Staining Protocol]

Whole Mount In Situ Hybridization of Mouse Protocol - http://www.hhmi.ucla.edu/derobertis/protocol_page/in_situ.html

Category: Mouse Protocols: Mouse Embryology Protocols

Protocol for whole mount in situ hybridization of mouse. Incluses: Preparation of the probe; General Comments; Preparation of embryos; In Situ hybridization. - [Read Whole Mount In Situ Hybridization of Mouse Protocol]

Isolation of Mouse Embryos Video Clip - http://www.swarthmore.edu/NatSci/sgilber1/DB_lab/Mouse/mouse_dissection.html

Category: Mouse Protocols: Mouse Embryology Protocols

Isolation of mouse embryos video clip. - [Read Isolation of Mouse Embryos Video Clip]

Aggregation of ES Cells with Mouse Morula- Stage Embryos Protocol - http://fds.oup.com/www.oup.co.uk/pdf/pas/13-10-6.pdf

Category: Stem Cell Protocols: Stem Cell Culture Protocols

Protocol for the aggregation of ES cells with mouse morula-stage embryos. Includes: Preparation of aggregation walls; ES cell preparation; Embryo preparation and aggregation. - [Read Aggregation of ES Cells with Mouse Morula- Stage Embryos Protocol]

Oviduct Transfer Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/18/pdb.prot4379

Category: Mouse Protocols: Mouse Reproductive System Surgery Protocols

Protocol describes oviduct transfer of mouse embryos. It is based on the Whittingham method, which applied a well described procedure for the rat to the mouse. It is best to practice this procedure first on a cadaver and then on an anesthetized 0.5-dpc pseudopregnant mouse using a dye solution or blue Affigel beads rather than embryos to gain experience in finding the opening of the oviduct (infundibulum). - [Read Oviduct Transfer Protocol]

Preparing Early Whole-Mount Drosophila Embryos for Immunostaining Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/23/pdb.prot4524

Category: Drosophila Protocols: Drosophila Staining Protocols

Early embryos (0-17 hours or until cuticle formation) are treated with a mixture of organic solvents, formaldehyde, and alcohols, as described here. The cuticles of late-stage embryos are usually opened by sonication. Tissues from more advanced stages of development are normally dissected by hand and then fixed and stained in a standard paraformaldehyde/detergent combination - [Read Preparing Early Whole-Mount Drosophila Embryos for Immunostaining Protocol]

FISH Protocols for Drosophila - http://www.utoronto.ca/krause/FISH.html

Category: Drosophila Protocols: Drosophila Genetics Protocols

FISH protocols for Drosophila. Includes: RNA Probe Preparation; Embryo Collection and Fixation; Single FISH on Drosophila embryos; Post-Fixation, Hybridization and Post-Hybridization Washes; Development of FISH Signal; Storage, Mounting and Viewing of Samples; Double FISH on Drosophila Embryos; RNA-Protein Double Labeling; FISH on Dissected Tissues. - [Read FISH Protocols for Drosophila]

Preparing Late Whole-Mount Drosophila Embryos for Immunostaining Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/23/pdb.prot4525

Category: Drosophila Protocols: Drosophila Staining Protocols

Early and late embryos are treated with a mixture of organic solvents, formaldehyde, and alcohols. The cuticles of late-stage embryos (17-22 hours or until hatching) are usually opened by sonication, as described here. Tissues from later stages of development are normally dissected by hand and then fixed and stained in a standard paraformaldehyde/detergent combination. - [Read Preparing Late Whole-Mount Drosophila Embryos for Immunostaining Protocol]

Making and Injecting Xenopus Embryos Protocol - http://spot.colorado.edu/~klym/Methods/embryos.htm

Category: Xenopus Protocols: Xenopus Injection Protocols

Protocol for making and injecting Xenopus embryos. Includes: Injections; Egg Activation and Inversion Experiments; - [Read Making and Injecting Xenopus Embryos Protocol]

Protocol for Nuclear Staining of Plants for Confocal Microscopy - http://www.cshprotocols.org/cgi/content/abstract/2007/1/pdb.prot4685

Category: Microscopy Protocols: Confocal Microscopy Protocols

Protocol describes a useful way to observe the development of embryos, as well as meristems & young primordia developing at the shoot apex by confocal microscopy after staining the nuclei with propidium iodide. The number of cells can be exactly quantified in a meristem or in young primordia. Because embryonic & meristematic cells are largely filled out by their nuclei, it is easier to image only the nuclei. This method allows analysis of whole-mount material, which is more easily reconstructed. - [Read Protocol for Nuclear Staining of Plants for Confocal Microscopy]

Whole Mount Fluorescent Antibody Staining of Drosophila Embryos Protocol - http://vosshall.rockefeller.edu/protocols/EmbryoImmunofluo.pdf

Category: Drosophila Protocols: Drosophila Staining Protocols

Protocol for whole mount fluorescent antibody staining of Drosophila embryos. - [Read Whole Mount Fluorescent Antibody Staining of Drosophila Embryos Protocol]

Sectioning Mouse Embryos Protocol - http://www.cshprotocols.org/cgi/content/abstract/2007/1/pdb.prot4703

Category: Mouse Protocols: Mouse Embryology Protocols

Protocol provides methods and tips for sectioning mouse embryos and transferring the sections to a microscope slide. - [Read Sectioning Mouse Embryos Protocol]

Fixation of Xenopus Embryos - http://tropicalis.berkeley.edu/home/manipulate_embryos/fixation.html

Category: Xenopus Protocols: Xenopus Embryo Manipulation Protocols

Protocol for the fixation of xenopus embryos. - [Read Fixation of Xenopus Embryos]

X-gal Staining of Wholemount Drosophila Embryos Protocol - http://jfly.iam.u-tokyo.ac.jp/html/manuals/pdf/E_Staining_Uemura.pdf

Category: Drosophila Protocols: Drosophila Staining Protocols

Protocol for X-gal staining of wholemount Drosophila embryos. - [Read X-gal Staining of Wholemount Drosophila Embryos Protocol]

Isolation of Fibroblasts from Chicken Embryos Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/18/pdb.prot4475

Category: Stem Cell Protocols: Stem Cell Isolation Protocols

This protocol describes the isolation of fibroblasts from chicken embryos. - [Read Isolation of Fibroblasts from Chicken Embryos Protocol]

Application of Direct Current Electric Fields to Cells and Tissues in vitro - http://www.natureprotocols.com/2006/12/12/application_of_direct_current.php

Category: Cell Biology and Cell Culture

Protocol applies EFs to cells in vitro but has been modified and to use electrotactic chambers to accommodate cells growing in planar culture or in three-dimensional (3D) gels, en bloc tissue cultures in 3D and possible small embryos, such as that from frog and zebra fish. The EF is applied to the cells or tissues cultured in a customer designed electrotactic chamber via agar salt bridges, Steinbergâ€™s solution and Ag/AgCl electrodes. - [Read Application of Direct Current Electric Fields to Cells and Tissues in vitro]

Whole Mount In Situ Hybridization for the Detection of mRNA in Drosophila Embryos Protocol - http://www.roche-applied-science.com/PROD_INF/MANUALS/InSitu/pdf/ISH_208-215.pdf

Category: Drosophila Protocols: Drosophila Genetics Protocols

Protocol for whole mount in situ hybridization for the detection of mRNA in Drosophila embryos. Includes: Embryo collection and fixation; Prehybridization treatment; Hybridization; Washing and detection. - [Read Whole Mount In Situ Hybridization for the Detection of mRNA in Drosophila Embryos Protocol]

Aggregation of ES Cells with Mouse Morula-state Embryos - http://www.oup.co.uk/pdf/pas/13-10-6.pdf

Category: Cell Biology and Cell Culture: ES Stem Cell Biology and Culture

Aggregation of ES Cells with Mouse Morula-state Embryos. Oxford Practical Series Approach. - [Read Aggregation of ES Cells with Mouse Morula-state Embryos]

Preparation of fixed Sea Urchin embryos for immunocytochemistry and AP staining - http://www.swarthmore.edu/NatSci/sgilber1/DB_lab/Urchin/Urchin_fix.html

Category: Developmental Biology: Sea Urchin Developmental Biology

Preparation of fixed Sea Urchin embryos for immunocytochemistry and AP staining. Cebra-Thomas. Swathmore. - [Read Preparation of fixed Sea Urchin embryos for immunocytochemistry and AP staining]

Protocol to Count Cell Number of Preimplantation Embryos - http://www.animal.ufl.edu/hansen/protocols/embryocellnumber.htm

Category: Developmental Biology: Embryology Protocols

Protocol to Count Cell Number of Preimplantation Embryos using Nuclear Staining with Hoechst 33342 or DAPI. Includes: Preparation of Embryos; Preparation of Hoechst 33342 dye; Preparation of DAPI; Staining the Embryo; Mounting Embryos to Slides; What to Do When There are Too Many Cells to Count. - [Read Protocol to Count Cell Number of Preimplantation Embryos]

Articles (1-1 of 1)

Acid Guanidinium Thiocyanate RNA Isolation Phenol Chloroform Extraction

A single step RNA isolation protocol using Phenol Chloroform Extraction and Acid Guanidinium Thiocyanate. This RNA isolation method uses the fact that guanidinium thiocyanate can simultaneously lyse the cells and inactive cellular RNAses during the initial RNA isolation step allow a single step in the method.

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