eliminates Protocols

Search results for: eliminates

Protocols » Search Results

Search Results Protocol Links Sort by: Hits | Alphabetical

Measurement of Cell Adhesion Under Static Conditions - http://www.glycotech.com/protocols/Proto8.html

Category: Cell Biology and Cell Culture: Cell Adhesion Protocols

Many proteins and molecules promote cell adhesion including several cell surface carbohydrate binding proteins. Cell adhesion measurements on 96-well microtiter plate format are difficult due to the shear forces generated by washing the wells. The protocol here introduces the use of a liquid-filled wash chamber that separates unbound cells by gravity. This eliminates uncontrolled shear forces and passage of adherent cells through a liquid/air interface. John L. Magnani~GlycoTech Corporation. - [Read Measurement of Cell Adhesion Under Static Conditions]

Preparation of Denaturing Polyacrylamide Gels Containing Formamide Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3807

Category: DNA Protocols: DNA Sequencing Protocols

The inclusion of formamide in sequencing gels eliminates secondarystructure in the DNA during electrophoresis. Formamide gelsare particularly useful and almost a necessity when sequencingDNA templates with a G/C content >55%. - [Read Preparation of Denaturing Polyacrylamide Gels Containing Formamide Protocol]

Preparation of Denaturing Polyacrylamide Gels Containing Formamide Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3807

Category: Nucleic Acid Electrophoresis Protocols

Protocol for preparation of denaturing polyacrylamide gels containing formamide.The inclusion of formamide in sequencing gels eliminates secondary structure in the DNA during electrophoresis. Formamide gels are particularly useful and almost a necessity when sequencing DNA templates with a G/C content >55%. - [Read Preparation of Denaturing Polyacrylamide Gels Containing Formamide Protocol]

Simplifying hESC culture - http://www.bloodjournal.org/cgi/content/full/105/12/4550

Category: Cell Biology and Cell Culture: ES Stem Cell Biology and Culture: ES Cell Growth Media and Requirements

Human embryonic stem cells are a valuable resource for research and cell replacement therapy but are notoriously cumbersome to culture. Bhatia and colleagues show that an increased dose of basic fibroblast growth factor eliminates the need for feeder laye - [Read Simplifying hESC culture]

Ultra-Fast Method of DNA Extraction from Neurospora Protocol - http://www.fgsc.net/fgn/camb.html

Category: Fungi Protocols: Neurospora Protocols

Simple method for growing Neurospora and for isolation of DNA that may be performed in two days from start to finish. The growth of mycelia in Petri plates eliminates the need for large numbers of flasks when growing many cultures for DNA isolation. - [Read Ultra-Fast Method of DNA Extraction from Neurospora Protocol]

Vegetative Propagation of Alfalfa by Stem Cuttings - http://www.oardc.ohio-state.edu/stockingerlab/Protocols/AlfalfaVegetativePropagationByStemCuttings.pdf

Category: Plant Biology Protocols

Alfalfa is an outcrossing species, cultivars consist of populations rather than individual homozygous or inbred lines. After only two cycles of self-pollination, severe inbreeding depression eliminates selfed individuals from populations. To obtain sufficient plant material of relative genetically uniformity (these plants will still necessarily be heterozygous) for experimental purposes, it may be necessary to propagate a single individual or clone. - [Read Vegetative Propagation of Alfalfa by Stem Cuttings]