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A non-radioactive electrophoretic mobility shift assay for the detection of heat shock element (HSE) - http://www.fgsc.net/fgn45/45meyer.html

Category: DNA Protocols: EMSA DNA-Protein Protocols

A non-radioactive electrophoretic mobility shift assay based on the digoxigenin detection system had been developed and applied to the analysis of the interaction between the heat shock transcription factor and the heat shock element of N. crassa. (Bioch - [Read A non-radioactive electrophoretic mobility shift assay for the detection of heat shock element (HSE)]

Inverse PCR & Cycle Sequencing of P Element Insertions for STS Generation Protocol - http://www.fruitfly.org/about/methods/inverse.pcr.html

Category: PCR Protocols: Inverse PCR Protocols

Protocol for inverse PCR & cycle sequencing of P element insertions for STS generation. - [Read Inverse PCR & Cycle Sequencing of P Element Insertions for STS Generation Protocol]

Isolation of Retroelement from Plant Genomic DNA - http://www.le.ac.uk/biology/phh4/retros.htm

Category: Plant Biology Protocols: Plant DNA RNA Isolation Protocols

Retroelements and their derivatives are a ubiquitous and abundant component of plant genomes. Major classes of retroelements include the Pseudoviridae (Ty1-copia ), the Metaviridae (Ty3 -gypsy) and the Retroposineae LINE (non-LTR) groups. All reverse transcribing elements can be included in a universal classification. Includes: Pseudoviridae (Ty1-copia) Degenerate Primers; Metaviridae (Ty3-gypsy) Element Degenerate Primers; LINE Element Degenerate Primers; PCR Programmes. - [Read Isolation of Retroelement from Plant Genomic DNA]

Screening DNA Pools for T-DNA Insertions in Arabidopsis Genes Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/28/pdb.prot4622

Category: Plant Biology Protocols: Arabidopsis Genetics Protocols

A powerful way to identify a mutation in the gene of interest and to test mutant plants for phenotypes that are predicted to result from loss of function of that gene is by PCR screening. Pools of insertion lines are screened using one primer corresponding to the gene of interest and one primer corresponding to the end of the insertion element. The synthesis of a product indicates the presence of an insertion in the gene of interest. - [Read Screening DNA Pools for T-DNA Insertions in Arabidopsis Genes Protocol]

Articles (1-6 of 6)

Genomic DNA Labeling of Microarrays Protocol

DNA microarrays are an ordered arrangement of DNA molecules complementary to genes of interest that are "spotted" by robotic equipment onto a glass slide substrate. The expression of genes in cells can be monitored with microarrays by preparing cDNA from the mRNA of cells of interest and measuring the hybridization to the microarray. This protocol describes the labeling of genomic DNA for use as a probe for hybridization to the cDNA spotted on the array.

Mast Cell Staining Protocol

A simple and concise mast cell staining protocol for cell histology.

Chrysoidin Stain

Chrysoidin Stain preparation method and protocol.

Crystal Violet Stain

Crystal Violet stain preparation method and protocol.

Thionin Staining

Thionin Staining method.

Vector Design Protocol for Transgenic Mice Generation

The protocol gives general considerations for the design of targeting vectors for transgenic mice. The protocol shares tips in the design of knock-out and knock-in vectors and some of their strategies for producing homologously recombined embryonic stem cells.

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