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Antibody Addition and Detection for Staining Caenorhabditis elegans Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/23/pdb.prot4523

Category: C. elegans Protocols: C. elegans Staining Protocols

Extreme care should be used to identify and verify positive reactions, however, because cross-reactions are common. Counterstaining is essential for examining worms by immunofluorescence and is used to identify the exact cell in which an antigen appears. Methods for counterstaining include labeling all cells with a fluorescent dye that is specific for nucleic acids (e.g., DAPI or propidium iodide) and using GFP driven by tissue-specific promoters. - [Read Antibody Addition and Detection for Staining Caenorhabditis elegans Protocol]

C. elegans EMS Mutagenesis Protocol - http://cobweb.dartmouth.edu/cgi-bin/cgiwrap/~ambros/protocol.cgi?id=26

Category: C. elegans Protocols: C. elegans Genetics Protocols

Protocol for C. elegans EMS mutagenesis. - [Read C. elegans EMS Mutagenesis Protocol]

C. elegans Gene Knockout Protocol - http://www.zoology.ubc.ca/~alorch/koprotocol.htm

Category: C. elegans Protocols: C. elegans Genetics Protocols

The procedure is to mutagenize a large population of worms with trimethylpsoralen and UV irradiation, set up 1152 subpopulations, screen DNA made from this library for deletions in specific genes by nested PCR, and then to recover single worms carrying the deletions through a sib-selection process. - [Read C. elegans Gene Knockout Protocol]

C. elegans Genomic DNA Prep Protocol - http://www.k-state.edu/hermanlab/protocols/genomic_dna_prep.htm

Category: C. elegans Protocols: C. elegans Genetics Protocols

Protocol for C. elegans genomic DNA prep. - [Read C. elegans Genomic DNA Prep Protocol]

C. elegans Gonad Fixation Protocol - http://www.genetics.wustl.edu/tslab/Protocols/gonad_fixation.html

Category: C. elegans Protocols: C. elegans Fixing Protocols

Protocol for C.elegans gonad fixation. Includes fixation with: Methanol, Formaldehyde, Methanol / Formadehyde, Glutaraldehyde / Formaldehyde. - [Read C. elegans Gonad Fixation Protocol]

C. elegans Integrating Extrachromasomal Arrays Protocol - http://cobweb.dartmouth.edu/cgi-bin/cgiwrap/~ambros/protocol.cgi?id=4

Category: C. elegans Protocols: C. elegans Genetics Protocols

Protocol and information related to C. elegans integrating extrachromasomal arrays. - [Read C. elegans Integrating Extrachromasomal Arrays Protocol]

C. elegans Integration via Gamma Radiation Protocol - http://www.biochem.northwestern.edu/ibis/morimoto/research/Protocols/IX.%20C.%20elegans/E.%20Genetics/1.%20Integration%20via%20gamma.pdf

Category: C. elegans Protocols: C. elegans Genetics Protocols

Protocol for C. elegans integration via gamma radiation. - [Read C. elegans Integration via Gamma Radiation Protocol]

C. elegans MMS Integration Protocol - http://www.k-state.edu/hermanlab/protocols/mms_integration.htm

Category: C. elegans Protocols: C. elegans Genetics Protocols

Protocol for C. elegans MMS integration. Includes: Henderson Method and Koelle Method . - [Read C. elegans MMS Integration Protocol]

C. elegans mRNA Prep Protocol - http://www.k-state.edu/hermanlab/protocols/joes_mrna_prep.htm

Category: C. elegans Protocols: C. elegans Genetics Protocols

Protocol for C. elegans mRNA prep. Includes: Oligo-dT cellulose prep; mRNA prep. - [Read C. elegans mRNA Prep Protocol]

C. elegans of F2 Mutant Screen - non "clonal" Protocol - http://cobweb.dartmouth.edu/cgi-bin/cgiwrap/~ambros/protocol.cgi?id=27

Category: C. elegans Protocols: C. elegans Genetics Protocols

Protocol for C. elegans F2 mutant screen - non "clonal". - [Read C. elegans of F2 Mutant Screen - non "clonal" Protocol]

C. elegans RNA Prep Protocol - http://www.k-state.edu/hermanlab/protocols/yale_rna_prep.htm

Category: C. elegans Protocols: C. elegans Genetics Protocols

Protocol for C. elegans RNA prep. - [Read C. elegans RNA Prep Protocol]

C. elegans RNAi Protocol - http://www.zoology.ubc.ca/~alorch/rnai-protocol.htm

Category: C. elegans Protocols: C. elegans Genetics Protocols

Protocol for C. elegans RNAi. Includes: Transformation of competent cells; Blunt-end ligation; Preparation of competent cells; Dephosphorylation of linear plasmid DNA; Restriction Digest: EcoRV; Insert amplification from gDNA; Gel purification: QiaQuick gel purification kit; Mini-prep; Transformant Screening; Bacterial preparation and induction; Preparation of worms for RNAi feeding. - [Read C. elegans RNAi Protocol]

C. elegans Worm Injection Protocol - http://cobweb.dartmouth.edu/cgi-bin/cgiwrap/~ambros/protocol.cgi?id=6

Category: C. elegans Protocols: C. elegans Injection Protocols

Protocol for C. elegans worm injection. - [Read C. elegans Worm Injection Protocol]

Cosuppression in C. elegans Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4318

Category: C. elegans Protocols: C. elegans Genetics Protocols

The cosuppression effect in C. elegans does not spread throughout the animal. Cosuppression in C. elegans can be triggered by highly repetitive transgenes that contain gene constructs. - [Read Cosuppression in C. elegans Protocol]

Cosuppression in C. elegans Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4318

Category: RNAi Technology Protocols: RNAi C. elegans Protocols

Cosuppression is a process in Caenorhabditis elegans that closely resembles RNAi. In contrast to RNAi, however, the cosuppression effect in C. elegans does not spread throughout the animal. Cosuppression in C. elegans can be triggered by highly repetitive transgenes that contain gene constructs. - [Read Cosuppression in C. elegans Protocol]

Detection of si/miRNA in C. elegans by Northern Blot Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4320

Category: RNAi Technology Protocols: RNAi C. elegans Protocols

Protocol uses northern blot technology to detect short interfering RNAs (siRNAs) or micro-RNAs (miRNAs) in RNA preparations from Caenorhabditis elegans. - [Read Detection of si/miRNA in C. elegans by Northern Blot Protocol]

Detection of siRNA in C. elegans Using Rnase Protection Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4319

Category: RNAi Technology Protocols: RNAi C. elegans Protocols

Protocol uses Rnase protection to detect short interfering RNAs (siRNAs) in RNA preparations from Caenorhabditis elegans. SiRNAs can also be detected by northern blot. However, the Rnase protection assay seems to be more sensitive. - [Read Detection of siRNA in C. elegans Using Rnase Protection Protocol]

Development of Techniques for Primary Culture of C. elegans Embryonic Neurons - http://cobweb.dartmouth.edu/cgi-bin/cgiwrap/~ambros/protocol.cgi?id=14

Category: C. elegans Protocols: C. elegans Cell Culture Protocols

Protocol for the development of techniques for primary culture of C. elegans embryonic neurons. - [Read Development of Techniques for Primary Culture of C. elegans Embryonic Neurons]

Development of Techniques for Primary Culture of C. elegans Embryonic Neurons 2nd Part - http://cobweb.dartmouth.edu/cgi-bin/cgiwrap/~ambros/protocol.cgi?id=15

Category: C. elegans Protocols: C. elegans Cell Culture Protocols

Development of techniques for primary culture of C. elegans embryonic neurons. Includes: Optimized cell culture protocol. - [Read Development of Techniques for Primary Culture of C. elegans Embryonic Neurons 2nd Part]

Development of Techniques for Primary Culture of C. elegans Embryonic Neurons 3rd Part - http://cobweb.dartmouth.edu/cgi-bin/cgiwrap/~ambros/protocol.cgi?id=16

Category: C. elegans Protocols: C. elegans Cell Culture Protocols

Development of techniques for primary culture of C. elegans embryonic neurons. - [Read Development of Techniques for Primary Culture of C. elegans Embryonic Neurons 3rd Part]

EM Fixation of C. elegans Embryos Protocol - http://www.aecom.yu.edu/wormem/embryo.htm

Category: C. elegans Protocols: C. elegans Fixing Protocols

Protocol for EM fixation of C. elegans embryos. - [Read EM Fixation of C. elegans Embryos Protocol]

EMS Mutagenesis of C. elegans Protocol - http://www.k-state.edu/hermanlab/protocols/ems_mutagenesis.htm

Category: C. elegans Protocols: C. elegans Genetics Protocols

Protocol for EMS mutagenesis of C. elegans. - [Read EMS Mutagenesis of C. elegans Protocol]

Fixation and Permeabilization of C. elegans Protocol - http://cobweb.dartmouth.edu/cgi-bin/cgiwrap/~ambros/protocol.cgi?id=35

Category: C. elegans Protocols: C. elegans Fixing Protocols

Protocol for fixation and permeabilization of C. elegans. Protocol works for all stages except dauers (which won't open) and hypoclorite-treated eggs (which disintegrate). Luckily, hypochlorite treatment and fixation are by themselves sufficient to open eggs. - [Read Fixation and Permeabilization of C. elegans Protocol]

Fixing Caenorhabditis elegans in Bouin’s Fixative Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/23/pdb.prot4521

Category: C. elegans Protocols: C. elegans Fixing Protocols

Bouin’s fixative is a particularly good choice for worms because it penetrates dense tissues well and is extremely good for fixing antigens. Like all strong fixatives, however, it is unsuitable for some antibody-antigen pairs. In such cases, the length of time in the Bouin’s fixative can be shortened, or paraformaldehyde fixation can be used instead. - [Read Fixing Caenorhabditis elegans in Bouin’s Fixative Protocol]

Fixing Caenorhabditis elegans in Paraformaldehyde Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/23/pdb.prot4522

Category: C. elegans Protocols: C. elegans Fixing Protocols

Common method for fixing worms is to use paraformaldehyde. This method provides a gentler fixation than the Bouin’s method, but often requires the use of collagenase. This method is particularly good for examining adult worms. - [Read Fixing Caenorhabditis elegans in Paraformaldehyde Protocol]

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