electrophoresis Protocols

Category: Proteomic Protocols: 2-D Gel Electrophoresis

A detailed protocol for 2-D Polyacrylamide Gel Electrophoresis using Prostate Tissue. Molecular Profiling Initiative. - [Read 2-D Polyacrylamide Gel Electrophoresis (Prostate Tissue)]

Category: Primary Cell Isolation and Culture Protocols: Laser Capture Microdissection Protocols

Protocol for 2-D polyacrylamide gel electrophoresis. Includes: 50 ml IEF Lysis Buffer; 10X Electrophoresis Running Buffer (10 L); 30% Acrylamide Stock (1 liter); Separating Acrylamide Gel; 50 ml Equilibration Buffer I; 50 ml Equilibration Buffer II; Transfer Buffer; Sample Preparation; Tissue Processing; Reswelling; Prepare Gradient Acrylamide Gel (9-18%); Transfer and Sequencing of Proteins. - [Read 2-D Polyacrylamide Gel Electrophoresis Protocol]

Category: Proteomic Protocols: 2-D Gel Electrophoresis

2D Gel Electrophoresis for Human Plasma Proteome. Lee Lab. - [Read 2D Gel Electrophoresis for Human Plasma Proteome]

Category: Chromatography Protocols: Affinity Chromatography Protocols

Recombinant protein or a chemically synthesized bioactive fragment is immobilized on resin and used as a probe to capture interacting proteins directly from a cell extract.  Affinity-purified proteins are fractionated by gel electrophoresis and visualized by Coomassie staining.  Proteins that interact specifically are identified by comparing this gel profile to one obtained from cell lysates passed over a control resin lacking the immobilized probe protein. - [Read Affinity Purification of Interacting Proteins from Cell Lysates Protocol]

Category: PCR Protocols: AFLP PCR

Restriction Digestion of Genomic DNA , Adapter preparation, Ligation of Adapters. Pre amplification Reactions. Gel electrophoresis, Silver Staining. AFLP Protocol and procedure. - [Read AFLP protocol]

Category: Molecular Biology Protocols: Agarose Gel Preparation

Agarose Gel Electrophoresis. Pouring of Agarose Gel, Preparation of samples, Gel electrophoresis, Ethidium bromide staining. - [Read Agarose Gel Electrophoresis PDF]

Category: Nucleic Acid Electrophoresis Protocols

Protocol on how to pour, load, and run an agarose gel. - [Read Agarose Gel Electrophoresis Protocol]

Category: Nucleic Acid Electrophoresis Protocols: DNA Electrophoresis Protocols: DNA Electrophoresis in Agarose Gels Protocols

Protocol for agarose gel electrophoresis of DNA. Includes: Making the gel; Loading the gel; Running the gel.
- [Read Agarose Gel Electroporesis of DNA Protocol]

Category: Nucleic Acid Electrophoresis Protocols: DNA Electrophoresis Protocols: DNA Electrophoresis in Agarose Gels Protocols

Alkaline agarose gels are used chiefly to measure the size of first and second strands of cDNA (Construction of cDNA Libraries Stage 1: Synthesis of First-strand cDNA Catalyzed by Reverse Transcriptase) and to analyze the size of the DNA strand after digestion of DNA-RNA hybrids with nucleases such as S1. - [Read Alkaline Agarose Gel Electrophoresis Protocol]

Category: Molecular Biology Protocols: Restriction Enzyme Digestion

Very detailed class protocol for Analysis of DNA by Restriction Enzyme Cleavage and Agarose Gel Electrophoresis. David Ng, UBC. - [Read Analysis of DNA by Restriction Enzyme Cleavage and Agarose Gel Electrophoresis]

Category: Cell Biology and Cell Culture: Apoptosis Protocols: Apoptosis Analysis

Analysis of DNA Fragmentation Using Agarose Gel Electrophoresis Shailaja Kasibhatla et al. This protocol provides a qualitative method for assessing cell death by detecting DNA fragments using agarose gel electrophoresis. One of the classic features of apoptosis is the cleavage of the genomic DNA into oligonucleosomal fragments represented by multiples of 180-200 bp. Visualizing these fragments can aid in characterizing an apoptotic event. May be combined with more quantitative methods. - [Read Analysis of DNA Fragmentation Using Agarose Gel Electrophoresis (Subscription Required)]

Category: Proteomic Protocols: 2-D Gel Electrophoresis

Analysis of Proteins using Small Format 2D Gel Electrophoresis. Cash Lab - [Read Analysis of Proteins using Small Format 2D Gel Electrophoresis]

Category: Yeast Protocols: Yeast Nucleic Acid Protocols: Yeast PCR Protocols

Yeast colonies are suspended in complete PCR buffer and transferred to a thermal cycler for 35 cycles of PCR. The products of the amplification reaction are analyzed by gel electrophoresis. - [Read Analyzing Yeast Colonies by PCR Protocol]

Category: Viral Manipulation Protocols: Phage Lamda Manipulation Protocols

Protocol describes the DNA content of bacteriophage lambda stocks can be easily and rapidly estimated. - [Read Assaying the DNA Content of Bacteriophage lambda Stocks and Lysates by Gel Electrophoresis Protocol]

Category: DNA Protein Interactions Protocols: Electrophoretic Mobility Shift Assays EMSA Protocols

Protocols on band shift. Includes: Phosphorylation of recombinant antibodies; Labeling the antigen with a red fluorophore; Native gel electrophoresis; Band-shift assay for the determination of Kd; Band-shift assay for the determination of koff. - [Read Band Shift Protocols]

Category: Buffers Media, and Solutions

Buffer Recipies for 2D-Electrophoresis - [Read Buffer Recipies for 2D-Electrophoresis]

Category: Molecular Biology Protocols: Agarose Gel Preparation

The quality of an RNA preparation may be assessed by electrophoresis on a denaturing agarose gel. - [Read Denaturing Agarose Gel Electrophoresis of RNA]

Category: Nucleic Acid Electrophoresis Protocols: RNA Electrophoresis Protocols

Protocol for denaturing agarose gel electrophoresis of RNA. Includes:  Prepare the gel; Prepare the RNA sample; Electrophoresis. - [Read Denaturing Agarose Gel Electrophoresis of RNA Protocol]

Category: Proteomic Protocols: 2-D Gel Electrophoresis

Excellent Detailed Protocol for 2-D Gel Electrophoresis including staining, gel running, and equipment. Purdue PSAL. - [Read Detailed 2D Gel Electrophoresis]

Category: Nucleic Acid Electrophoresis Protocols: Pulsed Field Gel Electrophoresis Protocols

Protocol for direct retrieval of DNA fragments from pulsed-field gels. A gel slice containing a fragment of DNA resolved by pulsed-field gel electrophoresis is treated with agarase.  The released DNA can be used as a substrate for ligation or restriction without further purification. - [Read Direct Retrieval of DNA Fragments from Pulsed-field Gels Protocol]

Category: DNA Protocols: DNA Sequencing Protocols

Methods for DNA sequencing.  Includes: Bst-catalyzed radiolabeled DNA sequencing; Radiolabeled sequencing gel preparation, loading, and electrophoresis; Taq-polymerase catalyzed cycle sequencing using fluorescent-labeled dye primers; Taq-polymerase catalyzed cycle sequencing using fluorescent-labeled dye terminator reactions; Sequenase[TM] catalyzed sequencing with dye-labeled terminators; etc. - [Read DNA Sequencing Methods]

Category: BACs Bacterial Artificial Chromosome Protocols

Sequencing conditions tested for the ABI Big-Dye terminators (PE-ABI #4303150 for the 1000 reaction kit - Description: TF,KIT BTD RR-1000) with various templates. Important to quantitate all templates by agarose gel electrophoresis vs size and concentration standards and do a few tests with different template concentrations to determine the optimal conditions for your reactions. Several conditions are given. - [Read Dye Protocols and Notes for Cosmid, BAC, BAC, Fosmid Templates]

Category: Nucleic Acid Electrophoresis Protocols: DNA Electrophoresis Protocols: DNA Electrophoresis in Agarose Gels Protocols

Protocol for electrophoresis of single stranded DNA using agarose gel. - [Read Electrophoresis - Agarose Gels for Single Stranded DNA Protocol]

Category: Molecular Biology Protocols: Agarose Gel Preparation

Electrophoresis of DNA in Agarose Gels. Frank Lab. - [Read Electrophoresis of DNA in Agarose Gels.]

Category: Plant Biology Protocols: Plant Proteins, Peptides and Antigens

Protocol describes a method for performing isoelectric fractionation of a maize embryo sample using a multicompartment electrolyzer(MCE). This prefractionation of proteins having pIs within a certain pH interval is essential for allowing high loads of protein to be resolved on narrow and ultra-narrow immobilized pH gradients used in 2D electrophoresis. The isoelectric membranes in the MCE act like isoelectric traps capturing all the protein species having pIs encompassing the pI value of each... - [Read Fractionation of Maize Embryo Proteins for 2-D Gel Electrophoresis Using Multicompartment Electrolyz]