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Apoptosis Detection Using TUNEL Enzymatic - http://www.ihcworld.com/_protocols/apoptosis/tunel_enzyme.htm

Category: Cell Biology and Cell Culture: Apoptosis Protocols: TUNEL assay apoptosis

Apoptosis Detection Using Terminal Transferase and Biotin-16-dUTP (TUNEL -Enzyme Method). IHC World - [Read Apoptosis Detection Using TUNEL Enzymatic]

Apoptosis Detection Using TUNEL Fluorescence - http://www.ihcworld.com/_protocols/apoptosis/tunel_fl.htm

Category: Cell Biology and Cell Culture: Apoptosis Protocols: TUNEL assay apoptosis

Apoptosis Detection Using Terminal Transferase and Biotin-16-dUTP (TUNEL - Fluorescence Method). IHC World - [Read Apoptosis Detection Using TUNEL Fluorescence]

Dealing with Carryover Contamination in PCR: An Enzymatic Strategy Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4092

Category: PCR Protocols

Protocol for dealing with carryover contamination in PCR- enzymatic strategy. Repeated use of PCR and manipulation of its products cause aerosols that can contaminate neighboring samples and work areas. Such "carryover contamination" can be prevented by including dUTP in place of dTTP for all amplification reactions. - [Read Dealing with Carryover Contamination in PCR: An Enzymatic Strategy Protocol]

Fluorescence In Situ Hybridization of a Repetitive DNA Probe to Human Chromosomes in Suspension - http://www.roche-applied-science.com/PROD_INF/MANUALS/InSitu/pdf/ISH_104-107.pdf

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Protocol for fluorescence in situ hybridization of a repetitive DNA probe to human chromosomes in suspension. Hybridization technique which does not need formamide and dextran sulfate. As a model system, we used the repetitive specific human DNA probe pUC 1.77, labeled it with digoxigenin-11-dUTP by nick-translation, and hybridized it to metaphase chromosomes in suspension. These chromosomes were isolated by standard techniques from human lymphocytes. - [Read Fluorescence In Situ Hybridization of a Repetitive DNA Probe to Human Chromosomes in Suspension]

Fluorescent In Situ Transcription in Cells and Tissues Protocol - https://catalog.invitrogen.com/index.cfm?fuseaction=iProtocol.unitSectionTree&treeNodeID=9E6639A7FEDD7A49BBC3758516F99557&objectid=6677C9E8B913BFB72E9F654025F3C39A

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Method assesses cellular mRNA transcripts in tissue sections and cell cultures using unique short anti-sense primers directed against sequences in particular protein(s). The unlabeled synthetic cDNA oligonucleotide primers are extended complementary to a sense mRNA transcript using reverse transcriptase and labeled through incorporation of a fluorescent-labeled dUTP nucleotide base. This procedure provides rapid detection of low abundance mRNA messages that can be related to other cellular.... - [Read Fluorescent In Situ Transcription in Cells and Tissues Protocol]

Immunoprecipitation of Homer 1a, Injection of Virions and In Situ Hybridization in the Spinal Cord - http://www.natureprotocols.com/2006/06/30/immunoprecipitation_ip_of_home.php

Category: Immunology: Immunoprecipitation Protocols: Protein Immunoprecipitation Protocols

Protocol for the immunoprecipitation (IP) of Homer 1a, injection of virions and in situ hybridization in the spinal cord. Includes: Immunoprecipitation (IP) of Homer1a from spinal cord; Injection of virions in the parenchyma of the spinal dorsal horn in vivo; Generation of cRNA probes; Analysis of DIG-dUTP incorporation; Tissue hybridization. - [Read Immunoprecipitation of Homer 1a, Injection of Virions and In Situ Hybridization in the Spinal Cord]

Nick Translation Labeling of Ds DNA with DIG-, Biotin-, or Flurochrome- Labeled dUTP Protocol - http://www.roche-applied-science.com/PROD_INF/MANUALS/InSitu/pdf/ISH_52-53.pdf

Category: Cytogenetics Protocols: DIG Biotin Labeled Probe Protocols

Protocol for nick-translation labeling of ds DNA with DIG-, Biotin-, or Fluorochrome-labeled dUTP. - [Read Nick Translation Labeling of Ds DNA with DIG-, Biotin-, or Flurochrome- Labeled dUTP Protocol]

Nick-Translation Labeling of ds DNA with DIG-, Biotin-, or Fluorochrome-Labeled dUTP Protocol - http://www.roche-applied-science.com/PROD_INF/MANUALS/InSitu/pdf/ISH_52-53.pdf

Category: Nucleic Acid Modification Protocols: DIG Biotin Labeled Probes Protocols

Protocol for nick-translation labeling of ds DNA with DIG-, Biotin-, or Fluorochrome-labeled dUTP. - [Read Nick-Translation Labeling of ds DNA with DIG-, Biotin-, or Fluorochrome-Labeled dUTP Protocol]

Nick-Translation Labeling of ds DNA with Nick Translation Mixes for In Situ Probes Protocol - http://www.roche-applied-science.com/PROD_INF/MANUALS/InSitu/pdf/ISH_49-51.pdf

Category: Nucleic Acid Modification Protocols: Nucleic Acid Labeling Protocols

Protocol for nick-translation labeling of ds DNA with nick translation mixes for in situ probes. Includes: Labeling reaction with DIG-dUTP or Biotin-dUTP; Labeling reaction with Fluorescein-dUTP, or Tetramethylrhodamine-dUTP; Purification of labeled probe. - [Read Nick-Translation Labeling of ds DNA with Nick Translation Mixes for In Situ Probes Protocol]

Oligonucleotide Tailing with a DIG-dUTP, Biotin-dUTP, or Fluorescein-dUTP Mixture Protocol - http://www.roche-applied-science.com/PROD_INF/MANUALS/InSitu/pdf/ISH_60-62.pdf

Category: Cytogenetics Protocols: DIG Biotin Labeled Probe Protocols

Protocol for oligonucleotide tailing with a DIG-dUTP, Biotin-dUTP, or Fluorescein-dUTP mixture. - [Read Oligonucleotide Tailing with a DIG-dUTP, Biotin-dUTP, or Fluorescein-dUTP Mixture Protocol]

Oligonucleotide Tailing With a DIG-dUTP, Biotin-dUTP, or Fluorescein-dUTP Mixture Protocol - http://www.roche-applied-science.com/PROD_INF/MANUALS/InSitu/pdf/ISH_60-62.pdf

Category: Nucleic Acid Modification Protocols: DIG Biotin Labeled Probes Protocols

Protocol for oligonucleotide tailing with a DIG-dUTP, Biotin-dUTP, or Fluorescein-dUTP mixture. - [Read Oligonucleotide Tailing With a DIG-dUTP, Biotin-dUTP, or Fluorescein-dUTP Mixture Protocol]

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Genomic DNA Labeling of Microarrays Protocol

DNA microarrays are an ordered arrangement of DNA molecules complementary to genes of interest that are "spotted" by robotic equipment onto a glass slide substrate. The expression of genes in cells can be monitored with microarrays by preparing cDNA from the mRNA of cells of interest and measuring the hybridization to the microarray. This protocol describes the labeling of genomic DNA for use as a probe for hybridization to the cDNA spotted on the array.