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96-well RNA In Situ Hybridization Protocol - http://www.fruitfly.org/about/methods/RNAinsitu.html

Category: RNA Protocols: cDNA Libraries Library

96-well RNA In Situ Hybridization Protocol. Berkeley Drosophila Genome Project - [Read 96-well RNA In Situ Hybridization Protocol]

96-well RNA In Situ Hybridization Protocol - http://www.fruitfly.org/about/methods/RNAinsitu.html

Category: PCR Protocols: In-situ PCR Protocols

96-well RNA In Situ Hybridization Protocol. Probe Preparation, Cell Inoculation, PCR, RNA Probe Preparation, etc, Very In-depth Protocol and Method Conditions. Berkeley Drosophila Genome Project. - [Read 96-well RNA In Situ Hybridization Protocol]

Antibody Addition to Drosophila Specimens and Detection Using Enzyme-Linked Reagents Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/23/pdb.prot4526

Category: Drosophila Protocols

Enzyme-linked reagents give excellent sensitivity and use a simple light microscope for detection. A range of enzymes is available, but for staining in situ, horseradish peroxidase will suit most needs. Diaminobenzidine (DAB) is one of the most sensitive substrates for horseradish peroxidase. It yields an intense brown product that is insoluble in both water and alcohol. It can be made more sensitive by adding metal salts such as cobalt or nickel to the substrate solution. - [Read Antibody Addition to Drosophila Specimens and Detection Using Enzyme-Linked Reagents Protocol]

Antibody Addition to Drosophila Specimens and Detection Using Fluorochrome-Linked Reagents Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/23/pdb.prot4527

Category: Drosophila Protocols

Protocol for antibody addition to Drosophila specimens and detection using fluorochrome-linked reagents. Fluorochrome-linked reagents should be used when high resolution is needed or if two antigens need to be localized simultaneously. Because of the thickness of fly specimens, detection requires access to a confocal microscope. - [Read Antibody Addition to Drosophila Specimens and Detection Using Fluorochrome-Linked Reagents Protocol]

Basic Methods of Culturing Drosophila Protocol - http://fly.bio.indiana.edu/Fly_Work/culturing.htm

Category: Drosophila Protocols: Drosophila Culture Media Protocols

Protocol for the basic methods of culturing Drosophila. Includes: Stockkeeping; Culture contaminants; Experimental populations. - [Read Basic Methods of Culturing Drosophila Protocol]

Chromatin Assembly on Template DNA with Transcription Factors and Drosophila S-190 Chromatin Assembl - http://www.bio.com/protocolstools/protocol.jhtml%3Bjsessionid%3D3APITJJIWFIM1R3FQLMCFEWHUWBNSIV0?id=p9061

Category: Drosophila Protocols: Drosophila Genetics Protocols

Protocol described here produces chromatin with regularly spaced nucleosomes having physiological nucleosome repeat lengths; something that can be difficult to achieve with purified components. In addition, chromatin assembled with the S-190 Chromatin Assembly Extract contains the ATP-dependent chromatin remodeling factors necessary for efficient transcription. - [Read Chromatin Assembly on Template DNA with Transcription Factors and Drosophila S-190 Chromatin Assembl]

Delivery of dsRNA into Drosophila Embryos by Gene Gun Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/21/pdb.prot4517

Category: RNAi Technology Protocols: RNAi Drosophila Protocols

Protocol describes how subcellular-sized particles are accelerated to high velocity to carry double-stranded RNA (dsRNA) into Drosophila embryos. The major advantage of this procedure over microinjection (Microinjection of dsRNA into Drosophila Embryos) is that particle bombardment is easier and faster to perform. In addition, the mechanical trauma received is far less than by microinjection, allowing better survival of embryos and fewer phenotypic artifacts. - [Read Delivery of dsRNA into Drosophila Embryos by Gene Gun Protocol]

Detection of Even-Skipped Transcripts in Drosophila Embryos with PCR/DIG-Labeled DNA Probes - http://www.roche-applied-science.com/PROD_INF/MANUALS/InSitu/pdf/ISH_216-219.pdf

Category: Drosophila Protocols: Drosophila Genetics Protocols

Protocol for detection of even-skipped transcripts in Drosophila embryos with PCR/DIG-labeled DNA probes. - [Read Detection of Even-Skipped Transcripts in Drosophila Embryos with PCR/DIG-Labeled DNA Probes]

Detection of Even-Skipped Transcripts in Drosophila Embryos with PCR/DIG-Labeled DNA Probes Protocol - http://www.roche-applied-science.com/PROD_INF/MANUALS/InSitu/pdf/ISH_216-219.pdf

Category: Cytogenetics Protocols: In Situ Hybridization Tissue Sections Protocols

Protocol for detection of even-skipped transcripts in drosophila embryos with PCR/DIG-labeled DNA probes. This protocol has been used to detect the transcript distribution of a number of genes by in situ hybridization, including evenskipped and seven-up, in whole mount Drosophila embryos, and engrailed Antennapedia in whole mount grasshopper embryos. Includes: Probe labeling; Evaluation of labeling reaction; Preparation of embryos, hybridization and detection. - [Read Detection of Even-Skipped Transcripts in Drosophila Embryos with PCR/DIG-Labeled DNA Probes Protocol]

Dissection of Drosophila CNSs Protocol - http://jfly.iam.u-tokyo.ac.jp/html/manuals/pdf/E_CNS_Dissection.pdf

Category: Drosophila Protocols: Drosophila Dissection Protocols

Protocol for the dissection of Drosophila CNSs. Includes: Tools; Dissection of the CNS; Dissecting larval CNSs; Dissecting early pupae; Dissecting mid pupae; Dissecting late pupae; Dissecting the whole CNS; Dissecting only the brain proper; Taking the pupa out of the puparium. - [Read Dissection of Drosophila CNSs Protocol]

Drosophila Non-fluorescent Protein and RNA Double-Labeling Protocol - http://www.utoronto.ca/krause/double%20labeling.html

Category: Drosophila Protocols: Drosophila Genetics Protocols

Protocol for Drosophila non-fluorescent protein and RNA double-labeling. - [Read Drosophila Non-fluorescent Protein and RNA Double-Labeling Protocol]

Drosophila Chromatin Assembly Protocol (S150 extract) - http://www.fhcrc.org/science/labs/tsukiyama/protocols/chromatin_assembly_S150_.pdf

Category: Drosophila Protocols: Drosophila Genetics Protocols

Protocol for Drosophila chromatin assembly (S150 extract). - [Read Drosophila Chromatin Assembly Protocol (S150 extract)]

Drosophila Cornmeal, Dextrose and Yeast Medium Protocol - http://fly.bio.indiana.edu/Fly_Work/media-recipes/dextrosefood.htm

Category: Drosophila Protocols: Drosophila Culture Media Protocols

Protocol for Drosophila cornmeal, dextrose and yeast medium. - [Read Drosophila Cornmeal, Dextrose and Yeast Medium Protocol]

Drosophila Cornmeal, Molasses and Yeast Medium Protocol - http://fly.bio.indiana.edu/Fly_Work/media-recipes/molassesfood.htm

Category: Drosophila Protocols: Drosophila Culture Media Protocols

Protocol for Drosophila cornmeal, molasses and yeast medium. - [Read Drosophila Cornmeal, Molasses and Yeast Medium Protocol]

Drosophila Cornmeal, Sucrose and Yeast Medium Protocol - http://fly.bio.indiana.edu/Fly_Work/media-recipes/harvardfood.htm

Category: Drosophila Protocols: Drosophila Culture Media Protocols

Protocol for Drosophila cornmeal, sucrose and yeast medium. - [Read Drosophila Cornmeal, Sucrose and Yeast Medium Protocol]

Drosophila Cornmeal, Sucrose, Dextrose, Yeast and 2-Acid Medium Protocol - http://fly.bio.indiana.edu/Fly_Work/media-recipes/caltechfood.htm

Category: Drosophila Protocols: Drosophila Culture Media Protocols

Protocol for Drosophila cornmeal, sucrose, dextrose, yeast and 2-acid medium. - [Read Drosophila Cornmeal, Sucrose, Dextrose, Yeast and 2-Acid Medium Protocol]

Drosophila Counting Protocol - http://flyrnai.org/RNAi_FAQ_dros_cc.html#VI.

Category: Drosophila Protocols

Protocol for Drosophila counting. - [Read Drosophila Counting Protocol]

Drosophila Embryo Microinjection Protocol - http://vosshall.rockefeller.edu/protocols/DrosophilaEmbryo.pdf

Category: Drosophila Protocols

Protocol for Drosophila embryo microinjection. - [Read Drosophila Embryo Microinjection Protocol]

Drosophila Fly Food Prep Method Protocol - http://fly.bio.indiana.edu/Fly_Work/media-recipes/method_press_n_seal.htm

Category: Drosophila Protocols: Drosophila Culture Media Protocols

Protocol for Drosophila fly food prep method. - [Read Drosophila Fly Food Prep Method Protocol]

Drosophila Food Protocol - http://vosshall.rockefeller.edu/protocols/Fly_Food_Protocol.pdf

Category: Drosophila Protocols: Drosophila Culture Media Protocols

Protocol for Drosophila food. - [Read Drosophila Food Protocol]

Drosophila Freezing Protocol - http://flyrnai.org/RNAi_FAQ_dros_cc.html#IV.

Category: Drosophila Protocols

Protocol for Drosophila freezing. - [Read Drosophila Freezing Protocol]

Drosophila Genomic DNA Preparation Protocol - http://vosshall.rockefeller.edu/protocols/Flygenomic.pdf

Category: Drosophila Protocols: Drosophila Nucleic Acids Protocols

Protocol for Drosophila genomic DNA preparation. - [Read Drosophila Genomic DNA Preparation Protocol]

Drosophila Growth Conditions Protocol - http://flyrnai.org/RNAi_FAQ_dros_cc.html#III.

Category: Drosophila Protocols: Drosophila Culture Media Protocols

Protocol for Drosophila growth conditions. Includes: MAINTENANCE; Semi-adherent cell lines; Adherent cell lines; SL2, S2C, S2*; DL2, DL1, S2R+, Kc167, Clone 8, S3; BG2. - [Read Drosophila Growth Conditions Protocol]

Drosophila Hard Agar Medium for Egg Collection Plates Protocol - http://fly.bio.indiana.edu/Fly_Work/media-recipes/hardagar.htm

Category: Drosophila Protocols: Drosophila Culture Media Protocols

Protocol for Drosophila hard agar medium for egg collection plates. - [Read Drosophila Hard Agar Medium for Egg Collection Plates Protocol]

Drosophila Larval Chemotaxis Assays - http://vosshall.rockefeller.edu/protocols/LarvalChemotaxisAssay.pdf

Category: Immunology: Chemotaxis

Drosophila Larval Chemotaxis Assays. Larva harvesting. Olfactory tests on control larvae. Measuring response. Leslie Vosshall Laboratory, Rockefeller University, New York. - [Read Drosophila Larval Chemotaxis Assays]

Articles (1-2 of 2)

Drosophila Cell Transfection Protocol

A simple Drosophila tissue culture cell transfection method.

Acid Guanidinium Thiocyanate RNA Isolation Phenol Chloroform Extraction

A single step RNA isolation protocol using Phenol Chloroform Extraction and Acid Guanidinium Thiocyanate. This RNA isolation method uses the fact that guanidinium thiocyanate can simultaneously lyse the cells and inactive cellular RNAses during the initial RNA isolation step allow a single step in the method.

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