Dried droplet Sample Preparation for MALDI.The discovery of this method allowed the application of laser desorption to proteins [2]. Drying a droplet of a protein/matrix solution remains the favorite method of most MALDI practitioners. Protocol for dried - [Read Dried droplet Sample Preparation for MALDI]
DNA Isolation from museum specimens: 1) what's the best tissue to sample; 2) what's the best way to get DNA out of that tissue? 1) the easiest well-dried tissue to get, and 2) the easiest extraction methods you are comfortable doing. Travis Glenn. - [Read Getting DNA from Old Dead Stuff]
Lipoplex (cationic liposome-DNA complex) is formed via electrostatic interaction of anionic nucleic acids with cationic liposomes. A thin film of lipids is dried on the bottom of a glass tube and rehydrated in an aqueous solution. The resulting liposome suspension is passed through polycarbonate filters of desired pore size. This protocol also describes the preparation, physical properties, and biological activity of liposome-polycation-DNA (LPD) nanoparticles. - [Read Lipoplex and LPD Nanoparticles for In Vivo Gene Delivery Protocol]
MALDI Matrices. Commonly used MALDI matrices for analysis of peptides, proteins, carbohydrates, and nucleic acids using 337 nm or 355 nm UV lasers. All matrices can be used for sample preparation using the Dried Droplet Method whereas only matrices solubl - [Read MALDI Matrices]
This method of sample preparation produces a uniform layer of very small crystals on the mass spectrometer's sample stage that are mechanically well adhered to the substrate. It is a variant of the dried droplet method. PROWL - [Read Polycrystalline thin films Sample Preparation Protocol MALDI]
This sample preparation method is a direct replacement for the dried droplet technique. It produces more uniform samples than the dried-droplet technique and the crystals are better adhered to the sample stage’s surface. MALDI Sample preparation. PROWL - [Read Seeded films (Vorm-Roepstorff) MALDI Protocol]