dose Protocols

Category: Cell Biology and Cell Culture: Cell Viability Protocols

Choosing a cell viability or cytotoxicity assay from among the many different options available can be a challenging task. Includes information on: Establishing an In Vitro Model System; Choosing an Endpoint to Measure; Characterizing Assay Responsiveness; Determining Dose and Duration of Exposure; Homogeneous Assays for Multiwell Formats and Automated Screening; Additional Factors to Consider When Choosing a Cell Viability Assay; Cell Viability Assays that Measure ATP Protocol; etc.. - [Read Cell Viability Information For Protocols and Applications]

Category: Cytogenetics Protocols

Cytogenetic protocol for blood preps. Includes: Preparation of materials; Culture Procedure - Use aseptic technique throughout procedure until harvest; Harvest; Slide making; Collecting blood by tail vein method; PHA Dose Response. - [Read Cytogenetic Protocol Blood Preps]

Category: Cell Biology and Cell Culture: Cell Transfection Protocol

What dose of Geneticin (G418) should I use for stable cell line selection? Invitrogen FAQs. - [Read Dose of G418 to be used for selection]

Category: Pharmacology and Toxicology Protocols: Cytotoxicity Protocols: Liver Toxicity Assays Protocols

The results of cytotoxicity tests in primary cultures of rat hepatocytes and in MDBK and McCoy cells can be used to predict the in vivo 4-wk maximum tolerated dose in rats and dogs.  A correlation between in vitro cytotoxicity, as measured in this system, and LD50 values in rats and mice has also been established. - [Read In Vitro Prediction of the Maximum Tolerated Dose Protocol]

Category: Pharmacology and Toxicology Protocols: Cytotoxicity Protocols

Mammalian toxicology for acute toxicity studies.  Includes: M101 Acute Limit Test (Rodents), M102 Acute Toxicity Study (Rats or Mice), M120 Dose Escalation Study (Dog), M103 Acute Dermal Limit Test, M104 Acute Dermal (LD50), Study M105 Primary Dermal Irritation, M106 Primary Eye Irritation Study, M106-A, M110 Local Lymph Node Assay in Mouse, M115 Rabbit Vaginal Irritation Assay. - [Read Mammalian Toxicology Acute Toxicity Studies]

Category: Pharmacology and Toxicology Protocols: Cytotoxicity Protocols

Mammalian toxicology repeat dose toxicity studies. Includes: M204 14-Day Oral Toxicity Study (Rodents), M204-H, M204-RH, M205 14-Day Oral Toxicity Study (Dogs), M205-H, M205-RH, M201 28-Day Oral Toxicity Study, M201-R, M202 28-Day Oral Toxicity Study, M202-R, M211 90-Day Oral Toxicity Study (Rodent), M211-20, M212 90-Day Oral Toxicity Study (Dog), M215 90-Day Dermal Toxicity Study, M213 Two-Year Chronic Toxicity/Carcinogenicity Study, M214 6-Month Carcinogenicity Study in p53+/Transgenic Mice. - [Read Mammalian Toxicology Repeat-Dose Toxicity Studies]

Category: Pharmacology and Toxicology Protocols: Cytotoxicity Protocols: Cytotoxicity Assays in Cell Culture Protocols

The cytotoxic effect of chemicals upon mammalian cells, such as BALB/c 3T3 and HepG2, in culture is measured by highest tolerated dose (HTD), cell viability (Neutral Red) and total cell protein (coomassie blue). - [Read Neutral Red Cytotoxicity Assay Protocol]

Category: Cell Biology and Cell Culture: ES Stem Cell Biology and Culture: ES Cell Growth Media and Requirements

Human embryonic stem cells are a valuable resource for research and cell replacement therapy but are notoriously cumbersome to culture. Bhatia and colleagues show that an increased dose of basic fibroblast growth factor eliminates the need for feeder laye - [Read Simplifying hESC culture]