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Contrast in Optical Microscopy - http://microscopy.fsu.edu/primer/techniques/contrast.html

Category: Microscopy Protocols: Optical Microscopy Protocols

When imaging specimens in the optical microscope, differences in intensity and/or color create image contrast, which allows individual features and details of the specimen to become visible. Contrast is defined as the difference in light intensity between the image and the adjacent background relative to the overall background intensity. In general, a minimum contrast value of 0.02 (2 percent) is needed by the human eye to distinguish differences between the image and its background. - [Read Contrast in Optical Microscopy]

Genotyping Protocol: T1320 mouse - http://www.mmrrc.org/strains/425/ctr_protocol.pdf

Category: Molecular Model Organisms: Mouse Animal Protocols: Mouse Genotyping Protocols

Genotyping Protocol: MMRRC 425. Assay Type: PCR - can distinguish heterozygous animals from homozygous animals. DNA Extraction: DNA from tail snips was ... - [Read Genotyping Protocol: T1320 mouse]

How to Distinguish a Spore Killer From Alternative Causes of Ascospore Abortion - http://www.fgsc.net/neurosporaprotocols/How%20to%20distinguish%20a%20Spore%20kill.pdf

Category: Fungi Protocols: Neurospora Protocols

Information and tips on how to distinguish a Spore killer from alternative causes of ascospore abortion. - [Read How to Distinguish a Spore Killer From Alternative Causes of Ascospore Abortion]

How to Distinguish Mitochondrial Mutations from Nuclear Mutations - http://www.fgsc.net/neurosporaprotocols/How%20to%20distinguish%20mitochondrial%20mutations%20from%20nuclear%20mutations.pdf

Category: Fungi Protocols: Neurospora Protocols

Information and tips on how to distinguish mitochondrial mutations from nuclear mutations. - [Read How to Distinguish Mitochondrial Mutations from Nuclear Mutations]

Measurement of Apoptosis and Other Forms of Cell Death - https://catalog.invitrogen.com/index.cfm?fuseaction=iProtocol.unitSectionTree&treeNodeID=9E6636C9D7EA88169E130B219EADFA39&objectid=6674C718AE37D97A78A88E365485C689

Category: Cell Biology and Cell Culture: Apoptosis Protocols: Apoptosis Analysis

Measurement of Apoptosis and Other Forms of Cell Death. Jagan Muppidi, Melissa Porter, and Richard M. Siegel. As programmed cell death (PCD) or apoptosis has emerged as an important regulator of development and homeostasis in multicellular organisms, methods to quantify apoptosis and to distinguish it from necrosis have been developed. This unit presents a set of assays for these purposes, many of which are technically very simple and ideally suited to the study of hematopoietic cells. - [Read Measurement of Apoptosis and Other Forms of Cell Death]

Random Amplification of Polymorphic DNA (RAPD) Typing and Fingerprinting Protocol - http://www.aspergillus.org.uk/indexhome.htm?secure/laboratory_protocols/./rapds.html~main

Category: Fungi Protocols: Aspergillus Protocols

RAPD is a procedure for typing and fingerprinting isolates of a species. It can be used for epidemiological studies, such as investigations into hospital outbreaks and as a laboratory aid to keep track of cultures and to verify that mutants generated in the laboratory are genetically identical to the parental strain. In our hands, the use of one primer, R108, is sufficiently discriminatory to distinguish between the isolates of different strains. - [Read Random Amplification of Polymorphic DNA (RAPD) Typing and Fingerprinting Protocol]